FastQCFastQC Report
Wed 30 Nov 2022
4061-JW20trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW20trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125643
Sequences flagged as poor quality0
Sequence length231
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA5577044.38766982641293No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA1380210.985092683237427No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA34882.7761196405689135No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGTGCGGTGCGACCGCATCTG24821.9754383451525352No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA22021.7525847042811775No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG17381.3832843851229277No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG10360.8245584712240236No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG7070.5627054432001782No Hit
GGAAAAGCGTTCATGGCGCAGATGTCCGAGACGTTGACGCTGATCGCCTT5520.43934003486067674No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC4530.36054535469544663No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT4250.33825999060831086No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA3580.284934297971236No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA3570.28413839211098113No Hit
TAGCAGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG3490.2777711452289423No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA3010.23956766393670956No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCATCAATTTGCTTGGTCGTAG2930.2332004170546708No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG2890.23001679361365138No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG2700.21489458226880923No Hit
TAGCCCTAAACGATGAGCGCTGGGTGTAGGGGGTATGAACCCCCCCTGTG2570.2045478060854962No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA2470.19658874748294775No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG2460.19579284162269286No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG2360.18783378302014436No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG2340.18624197129963468No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGAA2330.18544606543937983No Hit
ACGCCCTAAACAATGTTCACTTGGTGTCGGCCGCTCATACTGGTCGGTGC2290.18226244199836045No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT2030.16156888963173435No Hit
ACGCCGTAAACGATGAATACTAGGTGTAGGGGGTATCGACCCCCCCTGTG2010.15997707791122467No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG2000.15918117205096982No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1970.15679345447020526No Hit
ACGCCCTAAACAATGTTCACTTGGTGTCGGCCGCTTATACTGGTCGGTGC1930.15360983102918588No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1880.14963030172791164No Hit
GGAAAGGCGTTCATGGCGCAGATGTCCGAGACGTTGACGCTGATCGCCTT1750.13928352554459858No Hit
ACGCCGTAAACGATGAGTGCTAAGTGTTGGAGGGTTTCCACCCTTCAGTG1570.1249572200600113No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA1560.12416131419975644No Hit
AGGGTGTAAACGCTGCTCGCTTGATGTTAGTCGGGCTCCGAGCCCAACTA1520.12097769075873706No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG1520.12097769075873706No Hit
ACACGGTAAACGATGAATGCTAGGTGTAGGGGGTATCGACCCCTTCTGTG1500.11938587903822737No Hit
TAGCAGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT1490.11858997317797251No Hit
ACGCCGGAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA1420.11301863215618857No Hit
ACGCGGTAAACGATGAATACTAGGTGTAGGGGGTATCAACTCCCCCTGTG1390.11063091457542402No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1330.10585547941389492No Hit
CAGCCGTAAACGATGAACACTAGGTGTGGGGGGTATCGACCCCTTCCGTG1300.10346776183313038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCTA500.0225.000022
CGTAACC252.6720954E-9225.000025
AGCGTTC650.0225.06
AACAATG800.0225.09
AACAATC100.0019028347225.09
ACAGTAA100.0019028347225.03
CACGGTA202.3794564E-7225.02
TTTAAAC100.0019028347225.05
AACCATG100.0019028347225.09
CCTAACA100.0019028347225.0225
TGGCCGT650.0225.01
GTAGACG152.1296182E-5225.06
CGTAGAC100.0019028347225.05
ACGGATG100.0019028347225.09
TCGTCAC100.0019028347225.0225
AACGATC100.0019028347225.09
AACTATT100.0019028347225.09
GACGATG100.0019028347225.09
TGTCGTC100.0019028347225.0225
CACCCTA100.0019028347225.02
Produced by FastQC (version 0.11.9)