FastQCFastQC Report
Wed 30 Nov 2022
4061-JW20trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW20trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125643
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA2436019.38826675580812No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA1978315.74540563342168No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA1602512.754391410583956No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA1421211.311414085941914No Hit
CAGCGAATTCGGTAAAGTCTTTAGCTTGACCTTCATATTGCTGTCTGACC26232.087661071448469No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA24631.960316133807693No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC15261.2145523427488998No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC13721.0919828402696528No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA7560.601704830352666No Hit
TACAGTTCCCCGAAGGACCTCCTCTTTCAAGGAGTTTAACTGCAATATCA7420.590562148309098No Hit
TACAGCTTCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA5850.4656049282490867No Hit
GCAGCAAGGCGATCAGCGTCAACGTCTCGGACATCTGCGCCATGAACGCT5720.45525815206577364No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC4730.37646347190054363No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA4520.3597494488351918No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC3460.27538342764817775No Hit
ACTAGCTCTCTACCCGAAGGCAGAGTCGTCCCCCTTTCAGGTTCCTACCA3400.2706079924866487No Hit
GCAGGTTACTCCAAAGGAGTATCTATAGCCCTTTCAGGTATATATGCCTA2890.23001679361365138No Hit
GAAGGCTCCCCGAAGGGTCGGTCTGCTTTCGCTTCCCTACCACCAACATG2830.22524135845212226No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC2760.21967001743033837No Hit
TACGACTCGCCGAAGCACCTCATCTTTCAATGAGTTTAGTCGCAATATCA2580.20534371194575107No Hit
GCAGCAAGGCGATCAGCGTCAACGTCTCGGACATCTGCGCCATGAACGCC2380.18942559474065407No Hit
TACAGCTCCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA2350.18703787715988954No Hit
GCACGAGTCCCGAAGGAAGACCTCACTTCTGAGGCTGTCTCGTACAGTTC2330.18544606543937983No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT2260.1798747244175959No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC2110.16793613651377315No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA2000.15918117205096982No Hit
GACGACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA1960.1559975486099504No Hit
GACGCCTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA1860.1480384900074019No Hit
GCTAACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGTTAGCATATCA1720.13689580796383405No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA1650.13132446694205008No Hit
CAGCTCGTCTAGCAAGACCTTCAATCTGGCCTTCACTCTGCTGTCGATCC1530.1217735966189919No Hit
GAGGGCTCCCCGAAGGGTCGGTCTGCTTTCGCTTCCCTACCACCCACATG1510.1201817848984822No Hit
TACACCTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA1500.11938587903822737No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCAATATCA1490.11858997317797251No Hit
CTTCCTGCCCCGAAGGGCTTCCCCGGTTAAGGGTAATTCAGGAGATGTCA1360.10824319699465947No Hit
CACTTTGTCCCCGAAGGGAAAGTCCTATCTCTAGGATTGTCAAAGGATGT1350.10744729113440461No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATATAACCGTCGCCCC1350.10744729113440461No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGTTTCCTACCACCTGTATG1320.10505957355364008No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC1260.10028413839211098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTAG950.0230.000029
GTGGCCT1000.0230.00002230
CTGGTAT252.3428584E-9230.000026
GTTACTC252.3428584E-9230.000025
ACTAGCT500.0230.000021
GCCGAAG18800.0230.000029
GAAAATC252.3428584E-9230.000023
TGTTAAG252.3428584E-9230.000026
GGAAAAT252.3428584E-9230.000022
GCTAACT252.3428584E-9230.000021
TGGTATT252.3428584E-9230.000027
AATCAGG350.0230.06
GCGAATT3300.0230.03
CAGCCGA750.0230.09
CTAGTCA1050.0230.04
TACGACT3250.0230.01
AGCGAAT3400.0230.02
AATTCGG3300.0230.06
TAGGCTC350.0230.02
GAATTCG3300.0230.05
Produced by FastQC (version 0.11.9)