FastQCFastQC Report
Wed 30 Nov 2022
4061-JW21trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW21trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91330
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA3115034.107084200153295No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA1284014.058907259389027No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA78088.54921712471258No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA41484.541771597503558No Hit
ACAGGTTTCTCCAAAGGAGAATCACTATCCCTTTCAGGTAGCTATGCCTG18892.068323661447498No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC14991.641300777400635No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC12451.3631884375342165No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA8830.9668236067009746No Hit
CAGCGAATTCGGTAAAGTCTTTAGCTTGACCTTCATATTGCTGTCTGACC6490.7106098762728567No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC5160.5649841235081572No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC3860.4226431621592029No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC3130.3427132377094055No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC2590.28358699222599365No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA2500.27373261797875836No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC2190.2397897733493923No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA1900.20803678966385633No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA1870.20475199824811124No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA1590.1740939450344903No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA1570.17190408409066024No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC1560.1708091536187452No Hit
CTTCCTGCCCCGAAGGGCTTCCCCGGTTAAGGGTAATTCAGGAGATGTCA1510.16533450125917004No Hit
GACGACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA1500.164239570787255No Hit
GAAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCAACATG1330.14562575276469944No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC1330.14562575276469944No Hit
GAAGGCTCTCTTACGAGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG1310.14343589182086938No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT1300.14234096134895435No Hit
CAGCTCGTCTAGCAAGACCTTCAATCTGGCCTTCACTCTGCTGTCGATCC1260.1379612394612942No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG1200.131391656629804No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT1150.12591700427022884No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATATAACCGTCGCCCC1140.12482207379831381No Hit
GCAGCAAGGCGATCAGCGTCAACGTCTCGGACATCTGCGCCATGAACGCC1090.11934742143873865No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT1010.11058797766341838No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCACTCTGCTGTCGCCCC1000.10949304719150334No Hit
GCTAACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGTTAGCATATCA1000.10949304719150334No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT990.10839811671958831No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT970.10620825577575824No Hit
CAGCTAGTCAAGCAGAGTCTTCAACCCGGCTATCATTCAGCTGTCTTATC960.10511332530384321No Hit
CAGCTAGTCAAGTAGAGTCTTCATCCCGACTATCATTCAGCTGTCTTATC920.10073360341618307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGCC252.3319444E-9230.114534
AATTCGG1100.0230.114536
TCGGTAA1100.0230.114539
CTGGCAA500.0230.114539
ATTGCCC252.3319444E-9230.114535
ATTCGGT1100.0230.114537
TCGTCTG550.0230.114535
GAATTCG1100.0230.114535
TCCTGTA252.3319444E-9230.114537
CAGCCGA550.0230.114539
CATTGTC151.9452267E-5230.114524
AATCCGG2250.0230.114526
GTGTCAG302.5465852E-11230.114525
AAATCAG800.0230.114525
TGTCTTT100.0017782251230.114528
TGTCCGA100.0017782251230.114526
TCTGGTA100.0017782251230.114528
GTTCCTT151.9452267E-5230.114525
GTTCTTG100.0017782251230.114527
CACAAGG151.9452267E-5230.114529
Produced by FastQC (version 0.11.9)