FastQCFastQC Report
Wed 30 Nov 2022
4061-JW22trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW22trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141421
Sequences flagged as poor quality0
Sequence length231
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA6718547.50708876333783No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA62364.4095289949866No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA41162.910458842746127No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG35082.480536836820557No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG31732.243655468424067No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA12840.907927394092815No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG9580.6774100027577233No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG8510.6017493865833221No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG8240.5826574553991274No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG7530.5324527474703191No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG7320.5176034676603899No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG6950.4914404508524194No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG6630.46881297685633677No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA5730.40517320624235437No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG5590.39527368636906823No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG5080.3592111496878116No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT5030.35567560687592364No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG4380.3097135503213808No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC4330.3061780075094929No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA4300.3040566818223602No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA3880.2743581222025017No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT3660.25880173383019495No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTGGTCG3450.24395245402026572No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA3330.23546715127173473No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG3130.2213249800241831No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG3100.21920365433705036No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA3100.21920365433705036No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG3030.21425389440040732No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG3020.21354678583802972No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA2990.21142546015089697No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG2820.19940461459047806No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG2740.19374774609145742No Hit
ATGCCGTAAACGATGCAGACTAGGTATCGCGGGGGTAAAACCCGGCGGCG2730.19304063752907985No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG2720.19233352896670225No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG2710.19162642040432468No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA2620.18526244334292644No Hit
ACGCCGTAAACGATGGGCACTTGGTATCGCGGGTATTGACCCCTGCGGTG2410.17041316353299724No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA2260.1598065350973335No Hit
ACACTGTAAACGATGTACACTAGGTGCTGGGGCGGATGCTTCAGTGCCGT2250.1590994265349559No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCCTGAAGGGCTTGGTCGTAGC2250.1590994265349559No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCATCGAG2210.15627099228544558No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG2130.1506141237864249No Hit
CAGCCGTAAACGATGTGCATTAGGTGTATCGGTGACCACGAGTCACCGAG2120.14990701522404734No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTGTCAGTGACCACGTGTCACTGAG2110.14919990666166977No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGTGGGCGCCCA2060.14566436384978185No Hit
ACACGGTAAACGATGAATGCTAGGTGTAGGGGGTATCGACCCCTTCTGTG2020.14283592960027153No Hit
ACACTGTAAACGATGTGCACTAGGTGCTGGGGCGGATGCTTCAGTGCCGG2000.14142171247551635No Hit
ACGCTGTAAACGATGTTCACTAGGTGTTGGGGGTATTGACCCTCTCAGTG2000.14142171247551635No Hit
CAGCTGTAAACGATGCTAGCTAGGTGTTGGGTCGGCCACGCGCCGCCCCA1990.14071460391313878No Hit
TAGCTGTAAACGATGTGAACTTGGTGTCGGTGGGGTAAAAGCCATCGGTG1960.13859327822600603No Hit
ACGCCGTAAACGATGAACACTAGTTGTGTGCGATACACAGTACGCGACTG1960.13859327822600603No Hit
ACGCCGTAAACGATGAGCACTAAGTGTCGGGCGAATGCTCGGCGCTGCAG1900.13435062685174054No Hit
ACGCTGTAAACGTTGTGTACTAGGTGTTAGTGACTTGTTCACTAGTGCCG1890.13364351828936297No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC1850.13081508403985265No Hit
ACGCTGTAAACGATGGGTACTGGGTTTCGGGGGTCTCAACCCTCTCGGAG1700.12020845560418891No Hit
CAGCTGTAAACGATGCAAACTAGGTGTTTGAACGGCCACGTGCCGTTCTA1700.12020845560418891No Hit
GTGCCGTAAACGATGTGGACTAGGTGTCGGCGACGCGAGTCGGCGGTGCC1640.11596580422992342No Hit
ACGCCGTAAACGATGAGAACTAGGTGTTGCGGGTATTGACCCCTGCAGTG1420.10040941585761662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGTT1550.0224.989768
TCGCCGT100.0019033467224.989751
ACGCCAT152.1305703E-5224.989751
CCCTATA100.0019033467224.98975225
TAAACCA100.0019033467224.989757
GCCATAA202.381039E-7224.989753
TAGCCAT152.1305703E-5224.989751
AGACGAT100.0019033467224.989758
GCACAGT202.381039E-7224.989751
CCATAAA202.381039E-7224.989754
AGCTGTC152.1305703E-5224.98975225
CGTACAC100.0019033467224.989755
CATAAAC202.381039E-7224.989755
CGTAAAT100.0019033467224.989755
ACGTAAA303.092282E-11224.989754
AACTATC152.1305703E-5224.989759
CGGTAAA650.0224.989754
TCGACAA100.0019033467224.98975225
CACCCTA100.0019033467224.989752
ACGACGT202.381039E-7224.989751
Produced by FastQC (version 0.11.9)