FastQCFastQC Report
Wed 30 Nov 2022
4061-JW22trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW22trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141421
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA4940534.93469852426443No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA1579711.17019395987866No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA119388.441462017663572No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC40972.8970237800609526No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC20361.4396730330007566No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC14951.0571273007544848No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA7980.5642726327773103No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC6550.4631561083573161No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC5960.42143670317703874No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC5610.39668790349382343No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT5410.38254573224627175No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC4970.3514329555016582No Hit
CAGCTAGTCAAGCAGAGTCTTCAACCCGGCTATCATTCAGCTGTCTTATC3820.2701154708282363No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG3720.26304438520446044No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC3550.25102353964404156No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA3450.24395245402026572No Hit
CAGCTAGTCAAGTAGAGTCTTCATCCCGACTATCATTCAGCTGTCTTATC3160.22344630571131588No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA3050.21566811152516246No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA2930.20718280877663145No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT2790.19728328890334534No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC2640.18667666046768158No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA2600.18384822621817126No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG2550.18031268340628337No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT2270.16051364365971107No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA2260.1598065350973335No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATATTGCTGTCGTCCC2220.15697810084782318No Hit
CTCCATTGTCCCGAAGGAAAGGCTTATCTCTAAGCCGGTCAATGGGATGT2160.1527354494735577No Hit
CTTCCTGCCCCGAAGGGCTTCCCCGGTTAAGGGTAATTCAGGAGATGTCA2110.14919990666166977No Hit
CAGCTCGTCTAGCAAGACCTTCAATCTGGCCTTCACTCTGCTGTCGATCC2070.14637147241215945No Hit
GCAGGTTCCTTCAAAGAAGGATCACTAGCCCTTTCAGGTAGCTATGCCTA2050.14495725528740427No Hit
ATGACGACCCCGAAGGGAGGGAGTGTTTCCACTCCTGTCCGTCACGGTTC2010.14212882103789395No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT1970.13930038678838363No Hit
CAGCTAGTCAAGCAGAGTCTTCAGCCCGGCTATCATTCAGCTGTCTTATC1970.13930038678838363No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT1950.13788616966362846No Hit
ACTGGAGTCCCCGAAGGGAAGGCCTCATCTCTGAGGCTGCCCCCAGCAGT1910.13505773541411814No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA1900.13435062685174054No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTAAC1830.12940086691509747No Hit
TACACCTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA1820.1286937583527199No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT1810.1279866497903423No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC1800.12727954122796473No Hit
GTGGACTAACCCTGCTTTCACAGGGCTGACCCCGCCCGAAGGCGGAGCTC1750.12374399841607682No Hit
GAAGGCTCTCTTACGAGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG1740.12303688985369923No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATACAGCTGTCTTATC1740.12303688985369923No Hit
GTGACAGCTCCTTGCGGAGAGGGTGGCTTTCACCACCTGTCTGCCCCTCT1650.116672912792301No Hit
CAGCTAATCCGGCAAAGTCTTCAACCTGGCCTTCATATTGCTGTCGGTCC1620.11455158710516826No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCACTCTGCTGTCGCCCC1600.11313736998041309No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCATTCTGCTGTCGCCCC1590.11243026141803553No Hit
ACAGCGTCGTATTGCTACGCTAGATCATCTCTGACCTATCACACTGTATG1580.11172315285565794No Hit
ATACCTGTCCGAAGAAGGGTGTATCTCTACACCTGTCAGGTACAGTTCAA1520.10748050148139245No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT1510.10677339291901486No Hit
CGGAAAATCAGGTAAAGTCATCAGCTTGACCTTCATATATCCGTCGCTCC1510.10677339291901486No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCATACTGCTGTCGCTCC1490.10535917579425969No Hit
CACCGGTCCAGCCGAACTGACCCACCTGTTTCCAGGCAGTACGACCGGGA1460.10323785010712695No Hit
GGCCGCGCCTATTGCTAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG1440.10182363298237179No Hit
ATAGGCTCCCCGAAGGGTCGGTCGCATCTCTGCTCCCTACCACCTATATG1420.10040941585761662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAAC151.9487807E-5230.076076
GATCCAG1000.0230.076075
ATTGTCT151.9487807E-5230.076075
GCCATTG252.3410394E-9230.076077
CAGATAA151.9487807E-5230.076075
TCGTCCA151.9487807E-5230.076075
GCCTATT350.0230.076077
ATAACCT151.9487807E-5230.076078
CTCCTGA350.0230.076076
CTAGCAA350.0230.076079
ACTATTG100.0017801458230.076057
AGTCCCG100.0017801458230.076056
CAAGTAG3000.0230.076059
CAGCCGA1550.0230.076059
TCCTGAA100.0017801458230.076058
GTCCTTG450.0230.076057
TTGTCGA202.1299275E-7230.076055
AATGCTC100.0017801458230.076055
GTGTCTT100.0017801458230.076057
GTCCAGA100.0017801458230.076057
Produced by FastQC (version 0.11.9)