FastQCFastQC Report
Wed 30 Nov 2022
4061-JW23trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW23trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116353
Sequences flagged as poor quality0
Sequence length231
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA4836241.56489304100453No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA72586.2379139343205585No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA70636.0703204902323105No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG24792.1305853738193257No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG19961.7154693046161251No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG17671.5186544395073613No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA13151.130181430646395No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG9800.8422644882383781No Hit
TAGCCGTAAACGATGGATACTAGGTGTTGTGAGTATCGACCCTCACAGTG8760.7528813180579788No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG8240.7081897329677791No Hit
ACGCCGTAAACGATGAGCACTAAGTGTCGGGCGAATGCTCGGCGCTGCAG7310.6282605519410759No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG6280.5397368353201035No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG5760.49504525022990387No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT5160.4434780366642888No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA5070.43574295462944657No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG4850.4168349763220544No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT4790.41167825496549293No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG4320.3712839376724279No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG4130.35495432004331645No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG3990.34292197021133963No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA3300.2836196746108824No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA2970.2552577071497942No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC2740.23549027528297506No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2690.23119300748584048No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA2640.2268957396887059No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG2490.21400393629730216No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA2490.21400393629730216No Hit
ACACTGTAAACGATGTACACTAGGTGCTGGGGCGGATGCTTCAGTGCCGT2450.21056612205959452No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG2420.20798776138131375No Hit
ACGCCGTAAACGATGAGAACTAAGTGTTGGCGCAAGTCAGTGCTGAAGTT2400.20626885426245994No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC2320.19939322578704458No Hit
TAGCAGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT2230.19165814375220236No Hit
ACGCCGTAAACGATGGGCACTTGGTATCGCGGGTATTGACCCCTGCGGTG2190.1882203295144947No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG2190.1882203295144947No Hit
ACGCCCTAAACGATGCCAACTAGGTGTTGGGGAAGGAGACTTCCTTAGTA2160.18564196883621395No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG2120.18220415459850625No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG2080.1787663403607986No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTGGTCG1940.16673399052882176No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG1920.16501508340996796No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA1720.1478260122214296No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG1700.1461071051025758No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTGTCAGTGACCACGTGTCACTGAG1690.14524765154314886No Hit
ACACGGTAAACGATGAATGCTAGGTGTAGGGGGTATCGACCCCTTCTGTG1650.1418098373054412No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGAA1600.13751256950830662No Hit
TAGCCCTAAACGATGAATACTAGGTGTTGGAGGATTCACTCCTTCAGCGT1580.13579366238945278No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG1580.13579366238945278No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG1550.13321530171117205No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA1410.12118295187919521No Hit
ACGCCGTAAACGATGAGCACTAGGTGTCGGGGTCGCAAGACTTCGGTGCC1390.11946404476034138No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA1340.1151667769632068No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG1290.1108695091660722No Hit
TAGCTGTAAACGATGTGAACTTGGTGTCGGTGGGGTAAAAGCCATCGGTG1270.10915060204721838No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCATCGAG1260.10829114848779148No Hit
ACGCCGTAAACGATGGATACTAGGTGTGGGAGGTATCGACCCCTTCCGTG1250.10743169492836455No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGTGGGCGCCCA1230.10571278780951072No Hit
TAGCAGTAAACGATGTAGACTTGGCGTCGGTGGGGTAAAATCCGTCGGTG1230.10571278780951072No Hit
TAGCAGTAAACGATGTAGACTTGGCGTCGGTGGGTTAAAAACCATCGGTG1220.1048533342500838No Hit
ACGCTGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA1220.1048533342500838No Hit
ACGCCCTAAACGATGGGTACTTGGTGTCGCGGGTATCGACTCCCGCGGTG1210.10399388069065688No Hit
ACGCCGTAAACGATGAGTGCTAAGTGTTGGAGGGTTTCCACCCTTCAGTG1200.10313442713122997No Hit
ACGCCGTAAACGATGTTCACTAGGTGTTGGGGGTATTGACCCCCTCAGTG1170.10055606645294922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG2000.0224.9979
CACGGTA252.6720954E-9224.9972
GCCATAA152.1293301E-5224.9973
AAACGTT2000.0224.9978
TAACCGA252.6720954E-9224.9977
TTGACTA1750.0224.997225
ACTGTAA750.0224.9973
TCATAGC252.6720954E-9224.997225
CTGATTT600.0224.997225
CGCCCTA1850.0224.9972
GTAACCG252.6720954E-9224.9976
CAGACGG450.0224.997225
CAGACAA450.0224.997225
AGCGTTC100.0019027315224.996986
AACAATC100.0019027315224.996989
AAACTAT1700.0224.996988
ACGCCGC100.0019027315224.996981
TTTACTA202.3788562E-7224.99698225
TAGACGA202.3788562E-7224.99698225
GGCCCTA400.0224.996982
Produced by FastQC (version 0.11.9)