FastQCFastQC Report
Wed 30 Nov 2022
4061-JW23trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW23trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116353
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA3829132.90933624401605No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA1279010.992411025070261No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA92247.927599632153877No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC23532.0222942253315344No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA16661.4318496300052428No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC16571.4241145479704005No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC10290.884377712650297No Hit
CTCCATTGTCCCGAAGGAAAGGCTTATCTCTAAGCCGGTCAATGGGATGT7950.6832655797443985No Hit
GTTCGCGCTCCCGAAGGCACTCCTCCCTTTCAAGAGGATTCGCGACATGT7780.668654869234141No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG5710.49074798243276924No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC5140.44175912954543506No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA5000.4297267797134582No Hit
CTTCCTGCCCCGAAGGGCTTCCCCGGTTAAGGGTAATTCAGGAGATGTCA4720.40566208004950455No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC4530.3893324624203931No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA4130.35495432004331645No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA3630.31198164207197066No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC3010.2586955213875018No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA2880.24752262511495193No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT2870.246663171555525No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC2730.23463082172354818No Hit
ATCCATTGCCCGAAGGAGAGCGCATCTCTGCGCCTGTCAATGGTATGTCA2590.22259847189157134No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC2360.20283104002475227No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC2360.20283104002475227No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT2310.1985337722276177No Hit
CAGCTAGTCAAGTAGAGTCTTCATCCCGACTATCATTCAGCTGTCTTATC2250.19337705087105617No Hit
CAGCTAGTCAAGCAGAGTCTTCAACCCGGCTATCATTCAGCTGTCTTATC2210.18993923663334852No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGTTTCCTACCACCTGTATG2180.18736087595506776No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATCCCTACCACCTGTATG1890.1624367227316872No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA1880.16157726917226028No Hit
ATGACGACCCCGAAGGGAGGGAGTGTTTCCACTCCTGTCCGTCACGGTTC1850.15899890849397952No Hit
ACAGCGTCGTATTGCTACGCTAGATCATCTCTGACCTATCACACTGTATG1730.14868546578085653No Hit
CTTCCTGCCCCGAAAGGCTTCCCCGGTTAAGGGTAATTCAGGAGATGTCA1700.1461071051025758No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT1590.1366531159488797No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA1520.13063694103289128No Hit
CTTCCTGCCCCGAAGGGCTTCCCCGATTAAGGGTAATTCAGGAGATGTCA1480.1271991267951836No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA1400.12032349831976828No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT1370.11774513764148753No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG1360.11688568408206063No Hit
CAGCTAGTCAAGCAGAGTCTTCAGCCCGGCTATCATTCAGCTGTCTTATC1350.11602623052263371No Hit
CGGAAAATCAGGTAAAGTCATCAGCTTGACCTTCATATATCCGTCGCTCC1330.11430732340377987No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCACTCTGCTGTCGCCCC1320.11344786984435296No Hit
CACTTTGTCCCCGAAGGGAAAGTCCTATCTCTAGGATTGTCAAAGGATGT1270.10915060204721838No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT1270.10915060204721838No Hit
CTTCCTGCTCCGAAGAGCTTCCCCAGTTACGGGTAATTCAGGAGATGTCA1210.10399388069065688No Hit
TACGACTCGCCGAAGCACCTCATCTTTCAATGAGTTTAGTCGCAATATCA1200.10313442713122997No Hit
GAAACTGTTTTCATATTGCTATGAAAAAGGATCTAGTTTCTTAGATCTTT1200.10313442713122997No Hit
TACACCTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA1180.10141552001237614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG100.0017815743229.997013
CAGCGCG100.0017815743229.997011
ATTAGGG100.0017815743229.99701230
ATTAGCT202.1319647E-7229.997011
AATCAGG850.0229.997016
ATAGGCT400.0229.997011
AGATAAC100.0017815743229.997016
CGCGGCT100.0017815743229.997013
CAGTTGT100.0017815743229.997012
AGGACAG100.0017815743229.997011
AATGGCT100.0017815743229.997011
TAAGTAA100.0017815743229.997019
GTAGGCT202.1319647E-7229.997011
TATGCTC100.0017815743229.997012
CTGACTG100.0017815743229.997019
CCAGATA100.0017815743229.997014
ATACTGG100.0017815743229.997013
GTGGAGA100.0017815743229.99701230
TCCAGAT100.0017815743229.997012
AATTGTG100.0017815743229.997014
Produced by FastQC (version 0.11.9)