FastQCFastQC Report
Wed 30 Nov 2022
4061-JW24trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW24trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117607
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA5608847.69103879871096No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA83107.065905940972901No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA80936.881393114355438No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA15421.3111464453646466No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC12451.058610456860561No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC10370.8817502359553429No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT6140.5220777674798269No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA6030.5127245827204163No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC4570.3885823122773304No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA4200.35712159990476755No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA4000.340115809433112No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC3870.32906204562653585No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC3530.3001522018247213No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT3130.2661406208814101No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG3010.25593714659841676No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA2740.2329793294616817No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC2700.2295781713673506No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA2590.22022498660794No Hit
ATGACGACCCCGAAGGGAGGGAGTGTTTCCACTCCTGTCCGTCACGGTTC2590.22022498660794No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT2460.20917122280136388No Hit
ACCAATTCCCCGAAGGACCTCCCCTTTCAGGGAGTTTAATTGGCATATCA2410.20491977518344995No Hit
GAAGGCTCCCTTACGGGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG2300.1955665904240394No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG2290.1947163009004566No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA2250.1913151428061255No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT2250.1913151428061255No Hit
GTAGCACCTTCGAAAGCTGAATCCATCTCTGGAAACGTGCAGCTACAGTT2210.18791398471179438No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT2140.18196195804671492No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA1920.16325558852789376No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC1880.15985443043356262No Hit
ACAGCGTCGTATTGCTACGCTAGATCATCTCTGACCTATCACACTGTATG1860.15815385138639707No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG1850.15730356186281427No Hit
GTGACAGCTCCTTGCGGAGAGGGTGGCTTTCACCACCTGTCTGCCCCTCT1820.15475269329206595No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC1750.1488006666269865No Hit
GCAGGGCCCTCTCGGACACGACGTTTCCGCCGCTTTACCCTGCATGTCAA1740.1479503771034037No Hit
ACAAGTGCCATTGCTGGAGGGGTACTTTCATACCCTGTCACTTGCCTTTC1730.14710008757982093No Hit
ACAGGCTCCCCGAAGGGTCGGTCACCTCTCGGATCCCTACTACCTGTATG1700.1445492190090726No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC1700.1445492190090726No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT1670.14199835043832426No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT1620.13774690282041036No Hit
CTCCATTGTCCCGAAGGAAAGGCTTATCTCTAAGCCGGTCAATGGGATGT1620.13774690282041036No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC1430.12159140187233752No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA1350.1147890856836753No Hit
GGCCGCGCCTATTGCTAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG1270.10798676949501307No Hit
CTTCCTGCCCCGAAGGGCTTCCCCGGTTAAGGGTAATTCAGGAGATGTCA1250.1062861904478475No Hit
CCACGCTCCCCGAAGGGTCGATCCACTTTCGCTTCTCTACCACGTGCATG1240.10543590092426472No Hit
TACAGCTTCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA1230.10458561140068194No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGTTTCCTACCACCTGTATG1210.10288503235351637No Hit
GCAAGCTCCCCGAAGGGTCGGTCTGCTTTCGCTTCCCTACCACTTGCATG1190.10118445330635081No Hit
ACACCTGCTCCCGAAGGAGGGACTCCTATTTCTAAGAGTTTACAGGTGCG1180.10033416378276802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTAG202.1319283E-7230.00002230
AGCCGTC100.0017815289230.000023
CAGCGAT2050.0230.000021
TTGGCGC100.0017815289230.000022
ACAAATT100.0017815289230.000021
CCCTCGA252.3428584E-9230.000027
CTCGTCC100.0017815289230.000024
ATTAGCT252.3428584E-9230.000021
TTGGACT100.0017815289230.00002230
GTCGAGT100.0017815289230.000027
CAAGTGT100.0017815289230.000022
TTGTGCC100.0017815289230.000023
CGAAAAA252.3428584E-9230.000021
CCAGGAC100.0017815289230.00002230
CATTGTC100.0017815289230.000024
GACGTCC202.1319283E-7230.000024
TTGTCTG550.0230.000025
ATTACAG100.0017815289230.00002230
TTGTCGA100.0017815289230.000025
GGTCCAG252.3428584E-9230.000025
Produced by FastQC (version 0.11.9)