FastQCFastQC Report
Wed 30 Nov 2022
4061-JW25trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW25trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100449
Sequences flagged as poor quality0
Sequence length231
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTGCAGTA2874728.618502921880758No Hit
ACGCTGTAAACGATGGGTGCTCGGTGTCGCGGGTATCGACCCCTGCGGTG73807.347011916494937No Hit
TAGCCGTAAACGATGGATACTAGGTGTTGTCTGTATCGACCCGGACAGTG64896.459994624137622No Hit
ACGCCCTAAACGATGCCAACTAGGTGTTGGGGAAGGAGACTTCCTTAGTA48444.822347659011041No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTGTCAGTGACCACGTGTCACTGAG34753.4594669932005293No Hit
TAGCCGTAAACGATGGAAACTAGGTGTTGCCCGTATCGACCCGGGCAGTG32923.2772849903931345No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGGGAAGGAGACTTCTTTAGTA18021.7939451861143465No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA15911.5838883413473503No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTGTCAGTGACCACGTGTCACTGAT9390.9348027357166323No Hit
ACGCAGTAAACGATGCATACCAGGTGTGGGATGGCGTTCGCGTCGTTCCG8040.8004061762685541No Hit
ACGCCGTAAACGATGAATGCTAGACGTTGGCGAGCATGCTCGTCAGTGTC7900.7864687552887535No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCAGTGACCACGAGTCACTGAG7650.7615805035391094No Hit
TAGCTGTAAACGATGGGCACTAGCTTGGGGTCTCCCTGTGTGATCCCAGG7640.7605849734691237No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA6860.682933628010234No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG6210.6182241734611593No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG6100.6072733426913159No Hit
TAGCCGTAAACGATGGAAACTAGGTGTTGGCCGTATCGACCCGGTCAGTG4950.4927873846429532No Hit
TGGCCGTAAACGATGGGTACTAGGTGTGGGGGGTGTCAACTCCTCCCGTG4830.480841023803124No Hit
TAGCTGTAAACGATGGATACTAGGTGTTGGCTGTATCGACCCAGTCAGTA4770.47486784338320936No Hit
ACGCCGTAAACGATGAGCACTAGGTGTCGGGGTCGCAAGGCTTCGGTGCC4010.3992075580642913No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG3580.3563997650549035No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG3560.354408704914932No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA3490.3474399944250316No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG3430.341466814005117No Hit
TAGCCGTAAACGATGGATACTAGGCGTTGTCCGTATCGACCCGGACAGTG3410.3394757538651455No Hit
ACGCCGTAAACGATGAGTGCTAGTTGTTGGGGTGCATGCACCTCAGTGAC3320.3305159832352736No Hit
ACGCCCTAAACGATGTCAACTAGGTGTTGGGGGAGGAGACTTCCTTAGTA3150.31359197204551564No Hit
TAGCCGTAAACAATGGATACTAGATGTTGACCAGATCGACCTGTGCAGTA3120.31060538183555836No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGGGAAGGAGACTTTCTTAGTA2990.2976634909257434No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG2940.2926858405758146No Hit
TAGCCGTAAACAATGGATACTAGATGTTGCACAGATCGACCTGTGCAGTA2730.27177970910611354No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGAGAAGGAGACTTCTTTAGTA2440.24290933707652637No Hit
TAGCCGTAAACGATGGGCACTAGGCAAGGGAGGTACCTATGTCCTTCCTT2440.24290933707652637No Hit
TAGCAGTAAACACTGCACACTAAACATTAGTACCTCCTCGAGAGGTATTG2100.20906131469701045No Hit
ACGCCGTAAACGATGAGTGCTAGGTGTTGGGGGCTTTCCCGCCCTCAGTG2060.20507919441706737No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGGGGAGGAGACTTCCTTAGTA2060.20507919441706737No Hit
ACGCCGTAAACGATGGATGCTAGCCGTTGGGCAGCTTGCTGTTCAGTGGC2020.2010970741371243No Hit
ACGCCGTAAACGATGGGTGCTAGGTGCTGCGGGCTTTGACCCCTGCGGTG1900.18915071329729513No Hit
ACGCTGTAAACGATGGATACTAGGTGTGGGAGGTATCGACCCCTTCCGTG1800.17919541259743751No Hit
ACGCTGTAAACGTTGTATACTAGGTGTTAGTGGCTTGTCCACTAGTGCCG1750.17421776224750868No Hit
ATGCTGTAAACGATGTTCATTAGGTGTAGGGGGTATCGACCCCCTCTGTG1690.1682445818275941No Hit
CAGCTGTAAACGATGCGCATTAGGTGTGTCAGTGACCACGTGTCACTGAG1640.1632669314776653No Hit
ACGCCGTAAACGATGAGTGCTAGGTGTTGGGGGCTTCCCGCCCTCAGTGC1600.15928481119772223No Hit
ACGCCGTAAACGATGGATACTAGGTGTAGGAGGTATCGACCCCTTCTGTG1560.15530269091777918No Hit
TAGCAGTAAACACTGTACACTAAACATCAGTACCTCCTCGAGAGGTATTG1510.15032504056785037No Hit
CAGCCGTAAACGATGTGCATTAGGTGTATCGGTGACCACGAGTCACCGAG1490.14833398042787882No Hit
ACGCCCTAAACGATGATCACTAGCTTATCGGAGTATCGACCCTCTGAGAG1480.14733845035789306No Hit
ACGCTGTAAACGATGGATGCTAGATGTTGGGGCGCTTGCGTCTCAGTGTC1460.14534739021792153No Hit
ACGCCGTAAACGTTGGGCACTAGGTGTGGGTCTCAACCAACGAGATCCGC1340.13340102937809237No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGGGGGCGCCCA1320.13140996923812084No Hit
ACGCTGTAAACGATGGGTACTAGATTTAGTGGGTCTTGACCCCCGCTGAA1310.13041443916813505No Hit
ACGCTGTAAACGGTGAATACTAGACGTGGGGAGTATCGACCCTCCCTGTG1300.1294189090981493No Hit
TAGCCGTAAACGATGGGCACTTGGTAAGGGGGATTCCTATGGTCCTCCTT1230.12245019860824896No Hit
ACGCCCTAAACGATGTCTACTAGTTGTTGGTGGAGTAAAATCCATGAGTA1210.12045913846827744No Hit
TAGCGGTAAACGATGGATACTAGGTGTTGTCTGTATCGACCCAGACAGTG1190.11846807832830591No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGGGAAGGAGACTTCCTTAGTA1180.11747254825832014No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA1170.11647701818833438No Hit
ACGCCGTAAACGATGAATGCTAGACGTTGGGGAGCTTAGCTCTTCAGTGT1160.1154814881183486No Hit
ACGCCCTAAACGATGTTCTCTAGCTTATCGGAGTATCGACCCTCTGAGAG1150.11448595804836284No Hit
TAGCCGTAAACGATGGATACTAAGTGCTGTGCGGTTTGACGAGAGTCGTC1150.11448595804836284No Hit
TGGCCGTAAACGATGGGTACTAGGTGTGGGGGGTGTCGACTCCCCCCGTG1110.1105038377684198No Hit
ACGCCGTAAACGATGCCAGCTCGGTGTCTGGGACCCGTTCAGGGTCCTGG1110.1105038377684198No Hit
TAGCCGTAAACACTGAGCACTGGAGCGGAGGGGTTTCGGCCTTTCCGCTC1070.10652171748847673No Hit
ACGCCGTAAACGATGATAACTAGCTGCCAGGGCTCTTAGAGCTTTGGTGG1050.10453065734850522No Hit
ACGCCCTAAACTATGGATACTCGACATACGCGATAAACTGTGTGTGTCTG1040.10353512727851945No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCT100.0019023938224.995031
TTAGGCT100.0019023938224.99503225
CTGTAAC100.0019023938224.995034
AGCCCTA600.0224.995032
TAGACGA100.0019023938224.995037
AGCCCGA100.0019023938224.995032
ACCGATG152.1285374E-5224.995039
TAAACTA950.0224.995037
GCTGCTA100.0019023938224.99503225
CTGACTA100.0019023938224.99503225
GGCCCTA800.0224.995032
ACCCTAA152.1285374E-5224.995033
TTGACTC100.0019023938224.99503225
CCATAAA100.0019023938224.995034
TTTGGTA202.3773464E-7224.99503225
CATAAAC152.1285374E-5224.995035
AACTATC152.1285374E-5224.995039
TGTCGCT100.0019023938224.99503225
TACACTG100.0019023938224.99503225
CACCCTA152.1285374E-5224.995032
Produced by FastQC (version 0.11.9)