FastQCFastQC Report
Wed 30 Nov 2022
4061-JW25trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW25trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100449
Sequences flagged as poor quality0
Sequence length236
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT2709426.972891716194287No Hit
GTTCGCGCTCCCGAAGGCACTCCGAGCTTTCACTCAGATTCGCGACATGT61576.129478640902349No Hit
CAGCTAGTCAAGTAGAGTCTTCATCCCGACTATCATTCAGCTGTCTTATC47154.693924279982877No Hit
GTGGCGGCTCCCGAAGGAGAAGCCCTGTTTCCAGGACGGTCCACCACAGT41784.159324632400522No Hit
CAGCAGGATTCCTGGCATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCAT18181.8098736672341187No Hit
GTTCGCGCTCCCGAAGGCACCCTTCCCTTTCAAGAAGGTTCGCGACATGT16941.686427938555884No Hit
ATGGCGGCCCCCGAAGGGGAATACCTGTTTCCAGATACGCCCACCACAGT15321.5251520672181904No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA14761.4694023832989875No Hit
GTTCGCGCTCCCGAAGGCACCCTTCTCTTTCAAGAAGGTTCGCGACATGT12491.2434170574122192No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC11671.1617835916733865No Hit
GTCCAGGCTCTCTTTCGAGCACTCCCACCTCTCAGCGGGATTCCTGGCAT11651.159792531533415No Hit
CAGCAGGGTTCCGGGCATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCAT11571.151828290973529No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTAAC9870.9825881790759491No Hit
GTCCAGGCTCTCTTTCGAGCACTCCCACCTCTCAGCAGGATTCCTGGCAT9650.9606865175362621No Hit
GTGGACGCCAGCCGAACTGAAGGACCCGATCTCTCGAGCCCATACGTCCC8550.8511782098378282No Hit
CAGCGGGATTCCTGGCATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCAT7180.7147905902497785No Hit
GCAGGTCACACCCGAAGGTAATCAGCCAGCTTTCACCGGCCTAGCCCCTG6810.6779559776603052No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTACC6570.6540632559806469No Hit
GTCCGCGCTCCCGAAGGCACTCTTCCCTTTCAAGAAGATTCGCGGCATGT4170.4151360391840635No Hit
GTTCGCGCTCCCGAAGGCACTCCTCCCTTTCAAGAGGATTCGCGACATGT4140.41214944897410627No Hit
AGCACACCCTCGAAGGCGACTTCATTTCCGAAGCTTGCAGTGCTATGTCA3750.3733237762446615No Hit
CTCACTGTCCCCCGAAGGGGCCGACCTAATCTCTTAGGTATTCAGTGGAT3710.3693416559647184No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGACATTCGTATCATGT3320.3305159832352736No Hit
CGGAAAATCAGGTAAAGTCATCAGCTTGACCTTCATATATCCGTCGCTCC3260.324542802815359No Hit
GTCCCGATTCCCTTTCGGGCACCCCCACCTCTCAGCAGGGTTCCGGGCAT3050.30363667134565797No Hit
ATACATGTCCCGAAGGAAAGACGTATCTCTACGCCGGTCATGCACAGTTC3000.29865902099572916No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGCAATTCGTATCATGT2980.29666796085575764No Hit
CACCTCTGCCCCGAAGGGAAGGTCCCGTTACGGACCGGTCAGAGGGATGT2920.29069478043584307No Hit
CTCCTGGCTCCTTGCGGCACGTCCATGCATCTCTGCTAGACTTCCAGGGA2850.28372606994594274No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA2820.28073947973598545No Hit
CACCTCTGCTCCGAAGAGGGACTCTATCTCTAGAGTTTACAGAGGGATGT2630.2618244084062559No Hit
CCGCAGGTTCTCCGAAGAGCACCCCGACCTTTCGACCAGGTTCCTGCGTT2470.24589592728648368No Hit
GTATCCGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT2430.24191380700654067No Hit
GTGGGAGCCAGCCGAACTGAAGGAATCCATCTCTGGAAACCAAACTCCCC2370.23594062658662604No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT2350.23394956644665452No Hit
GTTCGCGCTCCCGAAGGCACTCCAAGCTTTCACTCAGATTCGCGACATGT2290.22797638602673992No Hit
CGAAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAATCGTCGCTCC2210.22001214546685383No Hit
GTGAACGCGTCCGAAGACAAGTCACCATCTCTGGTGATCATACGTTCCAT2180.2170255552568965No Hit
ACAGCACCCTCGAAGGCGACTTCATTTCCGAAGCTTGCAGCTGTATGTCA2170.21603002518691075No Hit
CAGCGTGTCAGGTAAGCCCTTCAGACTGACCTTCATCCTGCTGTCGCTCC2160.215034495116925No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC1920.19114177343726668No Hit
CCAATTGTCCTTGTGAGAAGGCTAAAGTTTCCTAAAGCTTTCAATTGAGT1730.17222670210753716No Hit
CCGAGTGTCCTTGCGGAAAACCACTTTCATGGTCGATCACTCGAAGTTCA1670.16625352168762256No Hit
CAGCGTGTCAGGTAAGCCCTTCAGACTGACCTTCATTCTGCTGTCGCTCC1620.16127587133769375No Hit
GTTCGCGCTCCCGAAGGCACTCTCCCCTTTCAAGGAGATTCGCGACATGT1620.16127587133769375No Hit
TACAGCTCCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA1590.15828928112773646No Hit
CTTGCCCTCCCTTGCGGGTCACCCCATGCTTCCATGAGGATCAGGCAGAT1590.15828928112773646No Hit
GTTCCGATTCCCTTTCGGGCACCCCCACCTCTCAGCAGGGTTCCGGACAT1460.14534739021792153No Hit
GTGGCGGCCCCCGAAGGAGAAGCCCTGTTTCCAGGACGGTCCACCACAGT1440.14335633007794998No Hit
GTTCGCGCTCCCGAAGGCACCCTCTGCTTTCACAAAGGTTCGCGACATGT1420.1413652699379785No Hit
GTCCAGGCTCTCTTTCGAGCACTCCCAAATCTCTTCAGGATTCCTGGCAT1410.14036973986799273No Hit
GCAGATTCCGACTCCGAAGAGCTGTCATCCCGCTTTCACGGGACTAATTC1400.13937420979800694No Hit
CACCTTGTCCCGAAGGACTTCCCATATCTCTATGGTATGCAAGGGATGTC1380.13738314965803541No Hit
CACTGATCCAGCCGAACTGAAGGAAAGTGTCTCCACTATCCGCGATCAGG1380.13738314965803541No Hit
GTCCAGGCTCCCTTTCGGGCACCAAGCCATCTCTGGCAAGTTCCTGGCAT1240.12344572867823472No Hit
GTATACGGTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT1210.12045913846827744No Hit
ACGCGGCCCCCGAAGGGACACTGGGTTTCCCCAGCTTTACCGCGCGGTTC1210.12045913846827744No Hit
CTGAAAATCTGGCAAGGTCATCAGCCTGGCCTTCATGTAACAGTCGCTCC1190.11846807832830591No Hit
GTTCAGATTCCCTTTCGGGCACGAATCCATCTCTGGAAACTTTCTGACAT1170.11647701818833438No Hit
ATCGGCATTGCTGCAACGAAGGGGGGTCATGCTCCAATTTGCTTCCGAGA1130.11249489790839133No Hit
CAGCGTGTCAGGTAAGCCCTTCAGACTGACCTTCATTCTGCTGTCGGCTC1070.10652171748847673No Hit
CTCCCGGTCGTATTGCTACGAAGACTGTATCTCTACAGCTGTCCGAGGAT1060.10552618741849099No Hit
GTGGCGGCTCCCGAAGGAGAAGCCCTGTTTCCAGGACGGTCCACCACAGG1010.10054853706856215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTAG10550.0230.107649
TTGTCCT450.0230.107645
CTAGTCA10250.0230.107644
GTCTGGT151.9460365E-5230.107627
TCCGACT500.0230.107627
TTGTCCC151.9460365E-5230.107625
TGTCCTT600.0230.107626
ACTGGTA151.9460365E-5230.107625
GGAGCTC100.0017786481230.10764
CAGCCGA1600.0230.10769
CGGTTCG100.0017786481230.10766
TTGTCTG100.0017786481230.10765
ATTGTCC400.0230.10764
CGTTCTA100.0017786481230.10766
TACGCTC202.1254164E-7230.10764
GTTGTAC100.0017786481230.10765
GTGTCCT100.0017786481230.10765
CAGTGTA100.0017786481230.10765
TTCGAAA100.0017786481230.10769
AGTGTAT100.0017786481230.10766
Produced by FastQC (version 0.11.9)