FastQCFastQC Report
Wed 30 Nov 2022
4061-JW26trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW26trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162051
Sequences flagged as poor quality0
Sequence length231
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA8578952.939506698508495No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA157619.725950472382152No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGTGCGGTGCGACCGCATCTG26211.6173920555874386No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA24461.5094013612998378No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA20151.243435708511518No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG15270.9422959438695225No Hit
TAGCCGTAAACTATGGATACTGGATCTTGCATATATTTGCTTGGTCGAAG13030.8040678551813936No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGAA8190.5053964492659718No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG8030.4955230143596769No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG7850.4844154000900951No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG6460.3986399343416579No Hit
TAGCAGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG5990.3696367193044165No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG5650.34865567012853976No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT4810.2968201368704914No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC4630.28571252260090957No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA3940.24313333456751268No Hit
CAGCTGTAAACGATGCTCGCTAGGTGTCAGGTGCGGTGCGACCGCATCTG3850.23757952743272182No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG3750.23140863061628744No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA3660.22585482348149657No Hit
TAGCCCTAAACGATGAGCGCTGGGTGTAGGGGGTATGAACCCCCCCTGTG3560.21968392666506226No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG3340.2061079536689067No Hit
GGAAAAGCGTTCATGGCGCAGATGTCCGAGACGTTGACGCTGATCGCCTT2980.18389272512974308No Hit
ACGCCGTAAACGATGAATACTAGGTGTAGGGGGTATCGACCCCCCCTGTG2970.18327563544809966No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCATCAATTTGCTTGGTCGTAG2870.17710473863166534No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG2830.1746363799050916No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT2750.16969966245194415No Hit
ACGCCCTAAACAATGTTCACTTGGTGTCGGCCGCTCATACTGGTCGGTGC2670.16476294499879668No Hit
ACGCTGTAAACGTTGGGTACTTGGTGTTGGGGGTTTGAGTCCCTCAGTGC2650.1635287656355098No Hit
TGGCTGTAAACGATGAGCACTAGGTGTCAGGGGCTTCGACCCCCTTGGCG2600.16044331722729263No Hit
ACGCCCTAAACAATGTTCACTTGGTGTCGGCCGCTTATACTGGTCGGTGC2580.15920913786400578No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA2580.15920913786400578No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG2480.15303824104757144No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA2450.15118697200264114No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG2430.14995279263935427No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG2400.148101523594424No Hit
ACACGGTAAACGATGAATGCTAGGTGTAGGGGGTATCGACCCCTTCTGTG2110.1302059228267644No Hit
ACGCTGTAAACGATCCCTGCTAGGTGTTCGTCTAGATTCTTATTTTCTCT2100.12958883314512099No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC1810.1116932323774614No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG1790.11045905301417455No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1710.1055223355610271No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1690.10428815619774022No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG1680.1036710665160968No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTTTCGCA1640.10120270778952306No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG1630.10058561810787961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAA4500.0225.000024
ATCTTTA152.1307553E-5225.00002225
AACGATA1200.0225.000029
ACGTAAA303.092282E-11225.000024
AACTATC350.0225.000029
AGCGTTC202.3815664E-7225.06
TTGGCAA100.0019033428225.0225
CACGGTA400.0225.02
CTATAAA100.0019033428225.04
ACCGATG100.0019033428225.09
TAGCCCT1350.0225.01
TGGCCGT850.0225.01
TAGCAGT2100.0225.01
AGTTGTA10300.0225.0225
GCCCTAA4400.0225.03
ACTCGTG100.0019033428225.0225
CGTACAC100.0019033428225.05
AGTTAGC800.0225.0225
CGTAACC100.0019033428225.05
CGTAAAT100.0019033428225.05
Produced by FastQC (version 0.11.9)