FastQCFastQC Report
Wed 30 Nov 2022
4061-JW26trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW26trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162051
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA5907836.45642421213075No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA2274114.033236450253314No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA99586.1449790498053085No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA99086.114124565723136No Hit
CAGCGAATTCGGTAAAGTCTTTAGCTTGACCTTCATATTGCTGTCTGACC24291.4989108367118995No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC11520.7108873132532352No Hit
ACAGGTTTCTCCAAAGGAGAATCACTATCCCTTTCAGGTAGCTATGCCTG10590.6534979728603958No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC9160.5652541483853848No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA7570.46713688900407896No Hit
GCTAACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGTTAGCATATCA6070.374573436757564No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA4090.2523896797921642No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC3600.22215228539163595No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA3350.20672504335055014No Hit
ACTAGCTCTCTACCCGAAGGCAGAGTCGTCCCCCTTTCAGGTTCCTACCA3230.19931996717082895No Hit
GAGGGCTCCCCGAAGGGTCGGTCTGCTTTCGCTTCCCTACCACCCACATG3120.19253198067275115No Hit
GAAGGCTCCCCGAAGGGTCGGTCTGCTTTCGCTTCCCTACCACCAACATG2960.18265854576645624No Hit
GCAGCAAGGCGATCAGCGTCAACGTCTCGGACATCTGCGCCATGAACGCT2830.1746363799050916No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGTTTCCTACCACCTGTATG2720.16784839340701385No Hit
CAGCGAATTCGGTAAGGTCTTTAGCCTGACCTTCATATTGCTGTCTGACC2680.1653800346804401No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG2650.1635287656355098No Hit
GCACGAGTCCCGAAGGAAGACCTCACTTCTGAGGCTGTCTCGTACAGTTC2570.15859204818236236No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC2530.1561236894557886No Hit
TACGACTCGCCGAAGCACCTCATCTTTCAATGAGTTTAGTCGCAATATCA2520.1555065997741452No Hit
GCAGGTTGCTCCAAAGGAGCATCTCTATCCCTTTCAGGTAGATATGCCTA2470.15242115136592801No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA2340.14439898550456337No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGTGAGTTTAGCTGCAATATCA2320.1431648061412765No Hit
GTAGCACCCTCGAAGGACCCCCCGTGTTTCCACAGGGATGCAGCTACAGT2240.13822808868812905No Hit
TACAGTTCCCCGAAGGACCTCCTCTTTCAAGGAGTTTAACTGCAATATCA2190.13514264027991188No Hit
GACGACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA2100.12958883314512099No Hit
GTAGGCTCCCCGAAGGGTCGGTCTACTTTCGTTTCCCTACCACCTACATG2100.12958883314512099No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT2070.1277375641001907No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC2040.1258862950552604No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC2030.12526920537361694No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATCCCTACCACCTGTATG2010.12403502601033008No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC1890.11662994983060888No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT1860.11477868078567859No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA1860.11477868078567859No Hit
CTCTCTGTCCCCGAAGGGAAAACGTAATCTCTTACGTCGTCAGAGGATGT1730.10675651492431394No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA1720.10613942524267052No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAATGAGTTTAGCTGCAATATCA1650.10181979747116648No Hit
GAAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCAACATG1650.10181979747116648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGGC1100.0230.000027
ACTCGCC11600.0230.000025
TCTGGCA550.0230.000028
TACAACT10850.0230.000021
CTTGTCA302.5465852E-11230.04
AGCGTCC600.0230.04
CCGGGCT151.9518788E-5230.03
AAATCAG302.5465852E-11230.05
CAAGTAG1400.0230.09
CAGCCGA1050.0230.09
GTCCTTG151.9518788E-5230.07
CAGCAAG252.3464963E-9230.02
GCACGAG500.0230.01
ATTCGGT3000.0230.07
TAAGTAA302.5465852E-11230.09
CGATTCT151.9518788E-5230.04
TATGCTC151.9518788E-5230.02
ACAGGCT450.0230.01
CCCCGAG350.0230.08
GTATGCT151.9518788E-5230.01
Produced by FastQC (version 0.11.9)