FastQCFastQC Report
Wed 30 Nov 2022
4061-JW27trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW27trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149568
Sequences flagged as poor quality0
Sequence length231
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA5677137.95664848095849No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA2175714.546560761660249No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA94376.309504706889174No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG18071.2081461275139067No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA14110.9433836114676936No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG11580.7742297817715019No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG11530.7708868207103123No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG11020.7367886178861789No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG9260.6191163885323063No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT8890.5943784766795036No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT7530.5034499358151476No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG6610.44193945228925974No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG5700.38109756097560976No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGTGCGGTGCGACCGCATCTG5660.3784231921266581No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC5310.3550224646983312No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG5150.3443249893025246No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG5020.3356332905434317No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGAA4510.3015350877192982No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG4090.27345421480530596No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG4000.26743688489516476No Hit
ACGCCGTAAACGATGAGCACTAAGTGTCGGGCGAATGCTCGGCGCTGCAG3650.24403615746683782No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA3540.2366816431322208No Hit
TAGCCGTAAACTATGGATACTGGATCTTGCATATATTTGCTTGGTCGAAG3440.22999572100984167No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG3210.2146181001283697No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG2940.19656611039794608No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC2820.18854300385109116No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG2770.18520004278990157No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG2700.1805198973042362No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG2670.17851412066752248No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2480.16581086863500213No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG2450.1638050919982884No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA2430.16246790757381258No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2410.16113072314933677No Hit
TAGCAGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG2400.16046213093709885No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG2310.15444480102695762No Hit
ACGCCGTAAACGATGAGAACTAGGTGTTGCGGGTATTGACCCCTGCAGTG2200.14709028669234062No Hit
GGAAAAGCGTTCATGGCGCAGATGTCCGAGACGTTGACGCTGATCGCCTT2170.14508451005562686No Hit
ACGCTGTAAACACTCCCTGTTAGATATTTGGTAGTTCATTGAAATTTTTT2140.14307873341891314No Hit
ACGCCGTAAACGATGAACACTAGTTGTGTGCGATACACAGTACGCGACTG2120.1417415489944373No Hit
ATGCTGTAAACGATGTGAACTAGGTGTTGCGCCGTCAAGGGGCAGTGCCG2070.13839858793324777No Hit
TAGCAGTAAACGATGTAGACTTGGTGTCGGAGGTGTCAAAACCTTCGGTA2060.13772999572100983No Hit
ACACGGTAAACGATGAATGCTAGGTGTAGGGGGTATCGACCCCTTCTGTG2030.1357242190842961No Hit
TAGCAGTAAACGATGTAGACTTGGCGTCGGTGGGGTAAAATCCGTCGGTG2020.1350556268720582No Hit
TAGCAGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT2010.1343870346598203No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA2010.1343870346598203No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA1940.1297068891741549No Hit
ACGCTGTAAACGTTGGGTACTTGGTGTTGGGGGTTTGAGTCCCTCAGTGC1940.1297068891741549No Hit
GTGCCGTAAACGATGTGGACTAGGTGTCGGCGACGCGAGTCGGCGGTGCC1940.1297068891741549No Hit
ACACTGTAAACGATGTACACTAGGTGCTGGGGCGGATGCTTCAGTGCCGT1890.12636392811296535No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1820.12168378262729995No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA1800.12034659820282415No Hit
ACGCCGTAAACGATGGGCACTTGGTATCGCGGGTATTGACCCCTGCGGTG1720.11499786050492083No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA1680.1123234916559692No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA1680.1123234916559692No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTGGTCG1650.11031771501925544No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG1630.10898053059477963No Hit
ACGCCGTAAACGATGAATACTAGGTGTAGGGGGTATCGACCCCCCCTGTG1580.10563756953359006No Hit
TAGCCCTAAACGATGAATACTAGGTGTTGGAGGATTCACTCCTTCAGCGT1560.10430038510911424No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGTGGGTTTAAATCCATCGGTG1550.10363179289687634No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA1540.10296320068463843No Hit
TGGCAGTAAACGATGTGAACTTGGCGTCGGCGGGATAAAACCTGTCGGTG1500.10028883183568678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAATG600.0224.997679
AACAATC202.3810753E-7224.997679
CTCCGTA100.0019032557224.997672
CACGGTA152.130516E-5224.997672
CAACGAT152.130516E-5224.997678
TAGACGG202.3810753E-7224.99767225
TAAACAA800.0224.997677
GGCCCTA450.0224.997672
GCCATAA152.130516E-5224.997673
TAGCCAT152.130516E-5224.997671
CCATCAA100.0019032557224.997674
CGAATTC800.0224.99767225
AACGATA2150.0224.997679
AAACAAT800.0224.997678
TCATAGC152.130516E-5224.99767225
CGGTAAA650.0224.997674
CTGATTG202.3810753E-7224.99767225
CGCCCTA3250.0224.997672
ATCTGTA400.0224.99767225
CCATTAG100.0019032557224.99767225
Produced by FastQC (version 0.11.9)