FastQCFastQC Report
Wed 30 Nov 2022
4061-JW27trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW27trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149568
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA4255228.449935815147626No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA2860719.126417415489943No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA109317.3083814719726155No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA43402.9016902011125376No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC15991.069078947368421No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC12710.849780701754386No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA10340.6913243474540008No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA7150.478043431750107No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG6420.4292362002567394No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC6080.40650406504065045No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC5060.3383076593923834No Hit
CAGCGAATTCGGTAAAGTCTTTAGCTTGACCTTCATATTGCTGTCTGACC5050.33763906718014547No Hit
CTCCATTGTCCCGAAGGAAAGGCTTATCTCTAAGCCGGTCAATGGGATGT4260.28482028241335045No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA4090.27345421480530596No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC4060.2714484381685922No Hit
TACAGCTTCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA3520.23534445870774498No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATCCCTACCACCTGTATG3310.22130402225074886No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT3260.21796106118955927No Hit
GCTAACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGTTAGCATATCA3250.21729246897732135No Hit
ACAGGTTTCTCCAAAGGAGAATCACTATCCCTTTCAGGTAGCTATGCCTG3150.21060654685494223No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT3130.2092693624304664No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC3060.20458921694480103No Hit
CTTCCTGCCCCGAAGGGCTTCCCCGGTTAAGGGTAATTCAGGAGATGTCA2880.19255455712451863No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT2850.19054878048780488No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGTTTCCTACCACCTGTATG2740.18319426615318785No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA2730.18252567394094993No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT2680.1791827128797604No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA2550.17049101412066753No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT2550.17049101412066753No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA2450.1638050919982884No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT2160.14441591784338897No Hit
GCAGGGCCCTCTCGGACACGACGTTTCCGCCGCTTTACCCTGCATGTCAA2160.14441591784338897No Hit
GTAGCACCTTCGAAAGCTGAATCCATCTCTGGAAACGTGCAGCTACAGTT2120.1417415489944373No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC2110.1410729567821994No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGCTTAGCTGCAATATCA2050.1370614035087719No Hit
ACAAGTGCCATTGCTGGAGGGGTACTTTCATACCCTGTCACTTGCCTTTC2040.13639281129653402No Hit
GCAGCAAGGCGATCAGCGTCAACGTCTCGGACATCTGCGCCATGAACGCT2040.13639281129653402No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT1980.13238125802310655No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC1940.1297068891741549No Hit
CACCGGTCCAGCCGAACTGACCCACCTGTTTCCAGGCAGTACGACCGGGA1870.1250267436884895No Hit
ACAGGCTCCCCGAAGGGTCGGTCACCTCTCGGATCCCTACTACCTGTATG1850.12368955926401369No Hit
GAAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCAACATG1840.12302096705177577No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGTGAGTTTAGCTGCAATATCA1840.12302096705177577No Hit
GAGGGCTCCCCGAAGGGTCGGTCTGCTTTCGCTTCCCTACCACCCACATG1740.11633504492939667No Hit
GGCCGCGCCTATTGCTAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG1720.11499786050492083No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC1710.11432926829268292No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC1680.1123234916559692No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG1620.10831193838254172No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAATGAGTTTAGCTGCAATATCA1600.10697475395806591No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA1600.10697475395806591No Hit
GAAGGCTCTCTTACGAGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG1530.10229460847240052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTC1450.0229.997683
CTTGTCT500.0229.997674
AAACTCG100.001782066229.997673
ACAAATT100.001782066229.997671
GCAAGTT302.5465852E-11229.997671
GCAAGGG151.9516576E-5229.997671
TTGGACT100.001782066229.99767230
ATAGGCT450.0229.997671
ATGGCTC252.3464963E-9229.997672
TTTACAA100.001782066229.99767230
AGATAAC100.001782066229.997676
CTATATT151.9516576E-5229.997679
GACGTCC400.0229.997674
ATCAGGT151.9516576E-5229.997677
TTGTCCT100.001782066229.997675
GTAGGCT252.3464963E-9229.997671
TCCTATA151.9516576E-5229.997677
AAAACTC100.001782066229.997672
CTGGTCC100.001782066229.997674
GTGTGTA100.001782066229.997675
Produced by FastQC (version 0.11.9)