FastQCFastQC Report
Wed 30 Nov 2022
4061-JW28trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW28trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127958
Sequences flagged as poor quality0
Sequence length236
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA3114624.34079932477844No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA1854914.496162803419871No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA113488.868534988042951No Hit
TACAGCTCCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA63604.970380906234858No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA42303.3057722065052593No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC20671.615373794526329No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC14991.1714781412651027No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT8420.6580284155738602No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC6720.5251723221682114No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC6610.5165757514184341No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA4750.37121555510401855No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA4360.3407368042638991No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC4250.33214023351412186No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC4100.3204176370371528No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA3970.3102580534237797No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT3840.30009846981040655No Hit
GAAGGCTCTCTTACGAGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG3460.27040122540208505No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA3350.2618046546523078No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC3100.24226699385735945No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA3060.23914096813016772No Hit
ACAGCGTCGTATTGCTACGCTAGATCATCTCTGACCTATCACACTGTATG2880.2250738523578049No Hit
TACGACTCGCCGAAGCACCTCATCTTTCAATGAGTTTAGTCGCAATATCA2840.22194782663061316No Hit
ACAAGTGCCATTGCTGGAGGGGTACTTTCATACCCTGTCACTTGCCTTTC2840.22194782663061316No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG2740.21413276231263384No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA2710.21178824301724006No Hit
GCAGCAAGGCGATCAGCGTCAACGTCTCGGACATCTGCGCCATGAACGCT2640.2063176979946545No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA2530.19772112724487723No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC2450.19146907579049374No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT2390.18678003719970615No Hit
GCAGGGCCCTCTCGGACACGACGTTTCCGCCGCTTTACCCTGCATGTCAA2370.1852170243361103No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT2320.1813094921771206No Hit
ACAGGCTCCCCGAAGGGTCGGTCACCTCTCGGATCCCTACTACCTGTATG2220.17349442785914126No Hit
GCTAACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGTTAGCATATCA2190.17114990856374748No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA2170.16958689570015162No Hit
ATACCTGTCCGAAGAAGGGTGTATCTCTACACCTGTCAGGTACAGTTCAA2140.1672423764047578No Hit
GAAGGCTCCCTTACGGGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG2090.16333484424576813No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT2060.16099032495037435No Hit
GGCCGCGCCTATTGCTAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG1890.14770471560980947No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT1880.14692320917801155No Hit
GTGACAGCTCCTTGCGGAGAGGGTGGCTTTCACCACCTGTCTGCCCCTCT1840.1437971834508198No Hit
GCAAGCTCCCCGAAGGGTCGGTCTGCTTTCGCTTCCCTACCACTTGCATG1830.14301567701902185No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG1770.13832663842823426No Hit
ACAGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCTGTATGTC1760.13754513199643634No Hit
GTAGCACCTTCGAAAGCTGAATCCATCTCTGGAAACGTGCAGCTACAGTT1700.13285609340564872No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC1670.13051157411025494No Hit
GTGGACTAACCCTGCTTTCACAGGGCTGACCCCGCCCGAAGGCGGAGCTC1670.13051157411025494No Hit
CAGCTAGTCAAGTAGAGTCTTCATCCCGACTATCATTCAGCTGTCTTATC1580.12347801622407352No Hit
ACTGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCAGTATGTC1550.12113349692867972No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCACTCTGCTGTCGCCCC1530.11957048406508386No Hit
CTCCCTGTCCCTTGCGGGAAAGCCTGCTTTCACAGGATTTCAGGGGATGG1490.11644445833789213No Hit
ATAGGCTCCCCGAAGGGTCGGTCGCATCTCTGCTCCCTACCACCTATATG1490.11644445833789213No Hit
GCAAGCTCCCCGAAGGGTCGGTCTGCTTTCACTTCCCTACCACTTGCATG1460.11409993904249831No Hit
CACCGGTCCAGCCGAACTGACCCACCTGTTTCCAGGCAGTACGACCGGGA1440.11253692617890244No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG1400.10941090045171072No Hit
GAAACTGTTTTCATATTGCTATGAAAAAGGATCTAGTTTCTTAGATCTTT1400.10941090045171072No Hit
ATGACGACCCCGAAGGGAGGGAGTGTTTCCACTCCTGTCCGTCACGGTTC1390.10862939401991278No Hit
CTTACGGCTCCCCGAAGGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT1350.10550336829272106No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCATTCTGCTGTCGCCCC1340.10472186186092311No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC1340.10472186186092311No Hit
CAGCTAGTCAAGCAGAGTCTTCAACCCGGCTATCATTCAGCTGTCTTATC1300.10159583613373138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACTC12550.0230.000022
AAATCAG650.0230.000025
AATCAGG650.0230.000026
AGCTTGT1100.0230.000022
GTGGGCT650.0230.00002230
GGTCCAG550.0230.000025
ACAGGCT550.0230.000021
GCAGGGC950.0230.000021
ACAAGCT550.0230.000021
TACAACT12600.0230.000021
AAACTGT151.9509222E-5230.02
CTGTATT302.5465852E-11230.09
GTCTGGT750.0230.07
CAAGTAG1200.0230.09
GCTGGCT151.9509222E-5230.01
TCCTGCT850.0230.03
ATAGCAC151.9509222E-5230.01
ACCTGCC151.9509222E-5230.03
TTCCGAT151.9509222E-5230.02
GCCATTG252.3446773E-9230.07
Produced by FastQC (version 0.11.9)