FastQCFastQC Report
Wed 30 Nov 2022
4061-JW29trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW29trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147289
Sequences flagged as poor quality0
Sequence length231
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA7444450.54281039317261No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA92706.293749024027592No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA30262.0544643523956303No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA29582.0082966141395486No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG15961.0835839743633267No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG13800.9369335116675379No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG13390.9090970812484299No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG12460.8459559098099654No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT10060.6830109512590893No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG8820.5988227226744699No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT8670.5886386627650402No Hit
ACGCCGTAAACGATGAGCACTAAGTGTCGGGCGAATGCTCGGCGCTGCAG8440.5730231042372479No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG8300.5635179816551135No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG6420.4358777641235938No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG6070.4121149576682577No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC5190.3523684728662697No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG4700.31910054382879915No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC4350.2953377373734631No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG4310.29262198806428175No Hit
ACACTGTAAACGATGTACACTAGGTGCTGGGGCGGATGCTTCAGTGCCGT3730.2532436230811534No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG3660.24849106179008615No Hit
TAGCAGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT3520.23898593920795172No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG3430.23287550326229386No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG3420.23219656593499852No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG3320.2254071926620454No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG3320.2254071926620454No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA3160.2145441954253203No Hit
ACGCCGTAAACGATGGGCACTTGGTATCGCGGGTATTGACCCCTGCGGTG3120.21182844611613902No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA3020.20503907284318582No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA2780.18874457698809824No Hit
ACGCTGTAAACACTCCCTGTTAGATATTTGGTAGTTCATTGAAATTTTTT2610.17720264242407782No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA2550.17312901846030593No Hit
ACGCCGTAAACGATGAACACTAGTTGTGTGCGATACACAGTACGCGACTG2480.1683764571692387No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA2360.1602292092416949No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG2330.15819239725980896No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG2200.14936621200496983No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA2180.1480083373503792No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG2140.1452925880411979No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG2100.14257683873201665No Hit
ACGCCCTAAACGATGGGTACTTGGTGTCGCGGGTATCGACTCCCGCGGTG2090.14189790140472133No Hit
ACGCCGTAAACGATGAGAACTAGGTGTTGCGGGTATTGACCCCTGCAGTG2070.1405400267501307No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2060.13986108942283537No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCCTGAAGGGCTTGGTCGTAGC2050.13918215209554008No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA1920.13035596684070094No Hit
TAGCTGTAAACGATGTGAACTTGGTGTCGGTGGGGTAAAAGCCATCGGTG1880.12764021753151966No Hit
TAGCCCTAAACGATGAATACTAGGTGTTGGAGGATTCACTCCTTCAGCGT1840.1249244682223384No Hit
TAGCAGTAAACGATGTAGACTTGGCGTCGGTGGGGTAAAATCCGTCGGTG1820.12356659356774777No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1820.12356659356774777No Hit
ACGCTGTAAACGTTGTGTACTAGGTGTTAGTGACTTGTTCACTAGTGCCG1710.11609828296749926No Hit
ACGCCGTAAACGATGAGAACTAAGTGTTGGCGCAAGTCAGTGCTGAAGTT1690.11474040831290863No Hit
TAGCAGTAAACGATGTAGACTTGGTGTCGGAGGTGTCAAAACCTTCGGTA1640.11134572167643204No Hit
TAGCAGTAAACGATGTAGACTTGGCGTCGGTGGGTTAAAAACCATCGGTG1620.10998784702184142No Hit
ACGCCGTAAACGATGAGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG1610.1093089096945461No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA1610.1093089096945461No Hit
ATGCCGTAAACGATGCAGACTAGGTATCGCGGGGGTAAAACCCGGCGGCG1600.10862997236725078No Hit
ACGCCGTAAACGATGAACACTAGTTGTGTGCGATACACTGTACGCGACTG1510.10251953642159291No Hit
ACGCTGTAAACGATGGGTACTGGGTTTCGGGGGTCTCAACCCTCTCGGAG1510.10251953642159291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAC4300.0225.000025
CGCCCTA3050.0225.000022
AACAATC100.001903167225.09
CTGTCAA152.1303618E-5225.04
ACAGTAA100.001903167225.03
CACGGTA100.001903167225.02
AGCCCTA650.0225.02
TCTTCAC100.001903167225.0225
ACCGATG252.6757334E-9225.09
TAAACCA100.001903167225.07
TAAACAA100.001903167225.07
TAAAAGA252.6757334E-9225.07
TGGCAGT400.0225.01
CTTATTT100.001903167225.0225
CGTAGAC100.001903167225.05
CCGAGCA100.001903167225.0225
CCATAAA202.3808389E-7225.04
AACGATA1400.0225.09
AAACAAT100.001903167225.08
ACTGTCA152.1303618E-5225.03
Produced by FastQC (version 0.11.9)