FastQCFastQC Report
Wed 30 Nov 2022
4061-JW29trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW29trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147289
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA6720845.630019892863686No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA99516.756105343915703No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA92846.303254146609727No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA13370.9077392065938393No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC10820.7346101881335334No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC10610.7203525042603317No Hit
CTCCATTGTCCCGAAGGAAAGGCTTATCTCTAAGCCGGTCAATGGGATGT8910.6049331586201278No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG7920.5377183632178913No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA7050.4786508157431988No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT6460.438593513432775No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA6410.43519882679629845No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA5570.37816809130349177No Hit
TACAGCTTCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA5070.3442212249387259No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT4380.297374549355349No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA4210.2858326147913286No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC4100.27836430419108016No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA3600.24441743782631425No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC3370.22880187929852194No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGTTTCCTACCACCTGTATG3250.22065463137097815No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT3220.2186178193890922No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC3090.20979163413425306No Hit
CTTCCTGCCCCGAAGGGCTTCCCCGGTTAAGGGTAATTCAGGAGATGTCA3080.20911269680695777No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA3070.20843375947966242No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC3030.20571801017048116No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA2960.20096544887941392No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG2930.198928636897528No Hit
ACAGCGTCGTATTGCTACGCTAGATCATCTCTGACCTATCACACTGTATG2830.1921392636245748No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT2810.19078138896998417No Hit
ATGACGACCCCGAAGGGAGGGAGTGTTTCCACTCCTGTCCGTCACGGTTC2710.183992015697031No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC2580.1751658304421919No Hit
GTAGCACCTTCGAAAGCTGAATCCATCTCTGGAAACGTGCAGCTACAGTT2550.17312901846030593No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATCCCTACCACCTGTATG2510.17041326915112467No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT2350.15955027191439958No Hit
ACAAGTGCCATTGCTGGAGGGGTACTTTCATACCCTGTCACTTGCCTTTC2240.15208196131415108No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC2230.15140302398685576No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC2210.15004514933226515No Hit
GCAGGGCCCTCTCGGACACGACGTTTCCGCCGCTTTACCCTGCATGTCAA2170.14732940002308387No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT2030.1378242774409494No Hit
GAAACTGTTTTCATATTGCTATGAAAAAGGATCTAGTTTCTTAGATCTTT1990.13510852813176816No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG1920.13035596684070094No Hit
GTGACAGCTCCTTGCGGAGAGGGTGGCTTTCACCACCTGTCTGCCCCTCT1840.1249244682223384No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCC1790.1215297815858618No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC1790.1215297815858618No Hit
ACAGGCTCCCCGAAGGGTCGGTCACCTCTCGGATCCCTACTACCTGTATG1770.12017190693127118No Hit
GCATGCTCCCCGAAGGGTCGATCCACTTTCGCTTCTCTACCACATGCATG1710.11609828296749926No Hit
ACACCTGCTCCCGAAGGAGGGACTCCTATTTCTAAGAGTTTACAGGTGCG1690.11474040831290863No Hit
GCAGGCTCCTTTCGGCCACGGCGTTTCCGCCGCTTCACCTGCATGTCAAG1690.11474040831290863No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA1680.11406147098561331No Hit
CTTACGGCTCCCCGAAGGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT1650.11202465900372736No Hit
GAAGGCTCCCTTACGGGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG1640.11134572167643204No Hit
GCAAAGCCCTTTCGGACACGACGTTTCCGCCGCTTTTCCTTGCATGTCAA1640.11134572167643204No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT1620.10998784702184142No Hit
CAGCTTGTCTGGTAAGGTCGTCAGCCTGACCTTCATTCTGCTGTCGCCCC1580.10727209771266015No Hit
GTGGACTAACCCTGCTTTCACAGGGCTGACCCCGCCCGAAGGCGGAGCTC1540.10455634840347888No Hit
GAAGGCTCTCTTACGAGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG1480.10048272443970696No Hit
ATAGGCTCCCCGAAGGGTCGGTCGCATCTCTGCTCCCTACCACCTATATG1480.10048272443970696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTGT100.0017819838230.000022
TGGAGTC100.0017819838230.000023
CAGCGAC100.0017819838230.000022
TCTTGTC100.0017819838230.000024
GACAGCG100.0017819838230.000021
AATCAGG1100.0230.000026
TGCCATT202.1337473E-7230.000026
GATCGCC100.0017819838230.000025
CGAGTGT202.1337473E-7230.000022
ATAGGCT550.0230.000021
GCTATTC100.0017819838230.000023
ATCAGGC950.0230.000027
ACTCTGT100.0017819838230.000022
GCCGGCT100.0017819838230.000021
ATTGTCC1100.0230.000025
TAAAAAT100.0017819838230.000022
GCCATTG202.1337473E-7230.000027
GTGGCGC100.0017819838230.00002230
CAGGGCC2200.0230.000022
TACGCTC202.1337473E-7230.000022
Produced by FastQC (version 0.11.9)