FastQCFastQC Report
Wed 30 Nov 2022
4061-JW30trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW30trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123978
Sequences flagged as poor quality0
Sequence length236
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA4851439.131136169320364No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA70885.717143364145252No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA50204.049105486457274No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC38843.1328138863346724No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC35212.8400200035490166No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC15851.2784526286921873No Hit
CAGCTAGTCAAGTAGAGTCTTCATCCCGACTATCATTCAGCTGTCTTATC11990.9671070673829227No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA10390.8380519124360772No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA9720.7840100663020858No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC7480.6033328493765023No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC7320.5904273338818178No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA6910.5573569504266885No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC5160.4162028747035765No Hit
CTCCATTGTCCCGAAGGAAAGGCTTATCTCTAAGCCGGTCAATGGGATGT4820.3887786542773718No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT4790.38635887012211845No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG3820.30811918243559344No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA3780.3048928035619223No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC3730.3008598299698334No Hit
CAGCTAGTCAAGCAGAGTCTTCAACCCGGCTATCATTCAGCTGTCTTATC3440.2774685831357176No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC3430.27666198841729983No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA3010.24278501024375293No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT2950.2379454419332462No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT2920.23552565777799286No Hit
ATGACGACCCCGAAGGGAGGGAGTGTTTCCACTCCTGTCCGTCACGGTTC2490.20084208488602814No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA2320.18712997467292583No Hit
CTTCCTGCCCCGAAGGGCTTCCCCGGTTAAGGGTAATTCAGGAGATGTCA2320.18712997467292583No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC2300.18551678523609028No Hit
GTAGCACCTTCGAAAGCTGAATCCATCTCTGGAAACGTGCAGCTACAGTT2230.1798706222071658No Hit
CAGCTCGTCTAGCAAGACCTTCAATCTGGCTTTCATACTGCTGTCGCTCC2150.17341786445982352No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTAAC2090.1685782961493168No Hit
CAGCTAGTCAAGCAGAGTCTTCAGCCCGGCTATCATTCAGCTGTCTTATC2020.16293213312039231No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATACAGCTGTCTTATC1940.15647937537305007No Hit
ATCCATTGCCCGAAGGAGAGGGCATCTCTGCCCCTGTCAATGGTATGTCA1910.15405959121779672No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT1850.14922002290729No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA1800.14518704931520107No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC1770.14276726515994773No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA1620.130668344383681No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCATACTGCTGTCGCTCC1590.12824856022842762No Hit
CAGCTAATCCGGCAAAGTCTTCAACCTGGCCTTCATATTGCTGTCGGTCC1550.12502218135475648No Hit
CAGCTCGTCTAGCAAGACCTTCAATCTGGCCTTCACTCTGCTGTCGATCC1540.1242155866363387No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG1530.12340899191792092No Hit
ATCCATTGCCCGAAGGAGAGCGCATCTCTGCGCCTGTCAATGGTATGTCA1510.12179580248108536No Hit
ACAAGTGCCATTGCTGGAGGGGTACTTTCATACCCTGTCACTTGCCTTTC1510.12179580248108536No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCACTCTGCTGTCGCCCC1460.11776282888899643No Hit
CGGAAAATCAGGTAAAGTCATCAGCTTGACCTTCATATATCCGTCGCTCC1460.11776282888899643No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATATTGCTGTCGTCCC1460.11776282888899643No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT1380.11131007114165417No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCATTCTGCTGTCGCCCC1350.10889028698640082No Hit
GTGACAGCTCCTTGCGGAGAGGGTGGCTTTCACCACCTGTCTGCCCCTCT1350.10889028698640082No Hit
CACCCTGTCCCGAAGGACTTCGCCCATCTCTGGGTTATGCAGGGGATGTC1350.10889028698640082No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATCGCTGTCGGTCC1330.10727709754956524No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATCTCTACCACCTGTATG1320.10647050283114746No Hit
GGCCGCGCCTATTGCTAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG1290.10405071867589412No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT1270.10243752923905854No Hit
ACAGCGTCGTATTGCTACGCTAGATCATCTCTGACCTATCACACTGTATG1270.10243752923905854No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT1260.10163093452064077No Hit
GCAAGTTCCTCCAAAGGAGGATCATGAGCCCTTTCAGGTCATTATGCTTG1260.10163093452064077No Hit
GCCGGCTCCCAGGAAACCTGGGTCCTCGCCCTTTCGGGTCCGTACCACCG1250.10082433980222298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCG100.0017816446230.000024
CTTCGTA100.0017816446230.000028
CTGTCTA100.0017816446230.000025
TTGGCGC100.0017816446230.000022
CTCACGG202.1323831E-7230.000021
AGTCCCG100.0017816446230.000026
GCAAGTT400.0230.000021
CGAAAAA400.0230.000021
ACGTTGT100.0017816446230.000023
ACGCACG100.0017816446230.000027
TAGACAG100.0017816446230.000021
TCCTGAA100.0017816446230.000028
TTTGTCC100.0017816446230.000024
ATCAGGC550.0230.000027
TTGTCGA100.0017816446230.000025
ACCTCTG202.1323831E-7230.000022
GTGTGTA100.0017816446230.000025
GTCAGAT100.0017816446230.000027
ACTAGCT100.0017816446230.000021
GTTGTAC202.1323831E-7230.000025
Produced by FastQC (version 0.11.9)