FastQCFastQC Report
Wed 30 Nov 2022
4061-JW31trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW31trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90504
Sequences flagged as poor quality0
Sequence length231
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCGTAAACGATGGATACTAGGTGTTGTCTGTATCGACCCGGACAGTG1606617.751701582250508No Hit
ACGCAGTAAACGATGCATACCAGGTGTGGGATGGCGTTCGCGTCGTTCCG1256013.877839653495977No Hit
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTGCAGTA68037.5167948377972245No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTGTCAGTGACCACGTGTCACTGAG24722.7313709891275524No Hit
TAGCCGTAAACGATGGAAACTAGGTGTTGCCCGTATCGACCCGGGCAGTG20882.307080350039777No Hit
ACACTGTAAACGATGAGCACTCGGTGTTGTGGGTGTCGACCCCTACAGTG20192.2308406258286926No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCAGTGACCACGAGTCACTGAG19672.1733846017855565No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG17821.9689737470167064No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTGTCAGTGACCACGTGTCACTGAT16661.8408026164589408No Hit
ACGCCGTAAACGATGAGCACTAGGTGTCGGGGTCGCAAGGCTTCGGTGCC16141.7833465924158047No Hit
TGGCCGTAAACGATGGGTACTAGGTGTGGGGGGTGTCAACTCCTCCCGTG12711.404357818438964No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG11771.3004950057456024No Hit
ACGCCCTAAACGATGCCAACTAGGTGTTGGGGAAGGAGACTTCCTTAGTA10521.1623795633342173No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGAGAAGGAGACTTCTTTAGTA8710.9623884027225317No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGGGAAGGAGACTTCTTTAGTA7340.8110138778396534No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG5900.6519048881817379No Hit
TGGCCCTAAACTGTGAACACTAGGCGTCGGGGGTATCGACCCCCTCGGTG5460.6032882524529303No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA5240.5789799345885265No Hit
ACGCCGTAAACGATGAGTGCTAGTTGTTGGGGTGCATGCACCTCAGTGAC4860.5369928400954653No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGGGAAGGAGACTTCCTTAGTA4820.5325731459383011No Hit
ACGCCGTAAACGATGAATGCTAGACGTTGGCGAGCATGCTCGTCAGTGTC3980.43975956863785026No Hit
ACGCCGTAAACGATGGAAGCCAGCCGTTGGCCAGCATGCTGGTCGGTGGC2960.32705736763015997No Hit
ACGCCCTAAACGATGTCGACTAGGTGTTCGGTGAGGAGACTCATTGAGTA2950.32595244409086893No Hit
ACGCCGTAAACGATGGGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG2930.32374259701228675No Hit
ACGCCCTAAACGATGTCAACTAGGTGTTGGGGGAGGAGACTTCCTTAGTA2890.31932290285512244No Hit
ACGCCCTAAACGATGTCAACTAGGTGTTGGGGAAGGAGACTTCTTTAGTA2690.29722443206930077No Hit
ACGCTGTAAACGTTGTATACTAGGTGTTAGTGGCTTGTCCACTAGTGCCG2620.28948996729426324No Hit
ACGCCGTAAACGATGTCAACTAACTGTTGGGTTCTTAAAGAACTTAGTAG2510.27733580836206134No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGGGGAGGAGACTTCCTTAGTA2500.27623088482277025No Hit
ACGCTGTAAACGATGGGTACTAGATTTAGTGGGTCTTGACCCCCGCTGAA2490.27512596128347916No Hit
CAGCTGTAAACGATGCGCATTAGGTGTGTCAGTGACCACGTGTCACTGAG2460.27181119066560594No Hit
ACGCTGTAAACGATGGATACTAGGTGTAGGAGGTATCGACCCCTTCTGTG2390.2640767258905684No Hit
ACGCCCTAAACGGTGCATACTAGGTCTTGGCGGATTCGACCCCGTCAGGG2380.2629718023512773No Hit
ACGCCGTAAACGATGTCAACTAACCGTTGGGTTCTTAAAGAACTTAGTGG2340.25855210819411295No Hit
ACGCTGTAAACGATGAGTGCTGGACGTTGGCGGGCTTGCCTGCCGGTGTC2260.24971271987978433No Hit
ACGCCCTAAACGATGTCTACTAGTTGTTGGTGGAGTAAAATCCATGAGTA2260.24971271987978433No Hit
ACGCCGTAAACGATGGATGCTAGCCGTTGGGCAGCTTGCTGTTCAGTGGC2210.2441881021833289No Hit
ACGCTGTAAACGATGTGTGCTAGACGTCGGGAGGCTTGCCTCTCGGTGTC2170.23976840802616461No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2120.23424379032970918No Hit
ACGCCGTAAACGATGAATGTTAGCCGTCGGCATGCATGCATGTCGGTGGC1780.19667638999381243No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGGGAAGGAGACTTTCTTAGTA1770.19557146645452134No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTTACCGAG1660.18341730752231947No Hit
ACGCTGTAAACGATGGATACTAGGTGTGGGAGGTATCGACCCCTTCCGTG1600.17678776628657297No Hit
ACGCTGTAAACGGTGGATACTAGGTGTGGAGGGTATCGACCCCCTCTGTG1590.17568284274728188No Hit
ACGCCGTAAACGATGTCAACTAGCCGTTGGTCCTATTTACAGGATTAGTG1530.1690533015115354No Hit
ACGCCGTAAACGATGTGTGCTAGCCGTTGGTGGCCCTTGAGGTCGTCAGT1520.16794837797224432No Hit
TAGCCGTAAACGATGTGAACTTGGCGTAGGTGGGTTCAAATCCATCTGTG1500.16573853089366217No Hit
ACGCAGTAAACGATGCATACCAGGTGTGGGCTGGCGTTCGCGTCGTTCCG1460.16131883673649783No Hit
ACGCCGTAAACGATGAATACTAGGTGTCAGCCCGCAGGGGTTGGTGCCGC1450.16021391319720674No Hit
ACGCCCTAAACGATGTTCTCTAGCTTATCGGAGTATCGACCCTCTGAGAG1440.15910898965791567No Hit
ACGCCCTAAACTATGGATACTCGACATACGCGATAAACTGTGTGTGTCTG1400.15468929550075133No Hit
ACACTGTAAACGATGGATGCTAGGTGTCGGGGAGTTCATCTTCGGTGCCG1250.13811544241138513No Hit
CAGCTTTTGAGATTGTAGGAAAGCGTCAGGCCGTAGCCGAATCCGGGGCC1230.13590559533280297No Hit
ACGCCGTAAACGATGAATGTTAGCCGTCGGGCAGTTTACTGTTCGGTGGC1190.13148590117563866No Hit
TAGCTGTAAACGATGAGTGCTGGGTGTAGGGGGTCATTACCCCCCCTGTG1160.12817113055776538No Hit
ACGCCGTAAACGATGAATGCCAGCCGTCGGGGAGCTTGCTCTTCGGTGGC1120.12375143640060107No Hit
TGGCCCTAAACGATGAGAACTGGGTAGTAGCCCGGGCATGGGGTTACTGC1120.12375143640060107No Hit
CAGCCGTAAACGATGTGCATTAGGTGTATCGGTGACCACGAGTCACCGAG1080.11933174224343676No Hit
ACGCCCTAAACGATGTCAACTAGGTGTTGGGAGGGTTAAACCTCTTGGTG1060.11712189516485459No Hit
ACACTGTAAACGATGTGCACTAGGTGCTGGGGCGGATGCTTCAGTGCCGG1030.11380712454698136No Hit
ACGCCGTAAACGATGAATGCTAGCCGTTGGCGAGCTTGCTCGTCAGTGGC1020.11270220100769027No Hit
ACGCCGTAAACGATGAGAACTAGGTGTTGCGGGTATTGACCCCTGCAGTG980.10828250685052593No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG970.10717758331123486No Hit
ACGCTGTAAACGATGAACACTTGGTGTGGTGGGAGTTGACCCCCGCCGTG970.10717758331123486No Hit
ACGCGGTAAACGATGGATGCTGGGTGTCGGGGGTTTACCTTCGGTGCCGC940.10386281269336162No Hit
ACGCAGTAAACGATGCATACCAGGTGTGGGATGGCTTTCGCGTCGTTCCG920.10165296561477945No Hit
ACGCCCTAAACGATGATAACTGGATTTAGGGAGTATCGACCCTCTCTGAG920.10165296561477945No Hit
ACGCTGTAAACGATGAGTGCTAGGCGTCGGCACGCTTGCGTGTCGGTGCC910.10054804207548838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT152.1276579E-5225.000028
AGCCCTA350.0225.000022
TAGCGGT152.1276579E-5225.000021
GGCCCTA1500.0225.000022
TAACCGA152.1276579E-5225.000027
CCATAAA152.1276579E-5225.000024
TACACTG152.1276579E-5225.00002225
GTAACCG152.1276579E-5225.000026
AACAATG8450.0225.09
CGGCGAT550.0225.0225
AAAAGAT400.0225.08
TAAACAA8450.0225.07
GGTCAAG100.0019019591225.0225
TAACGAT100.0019019591225.08
AAAAAGA100.0019019591225.07
GCCCTAA9400.0225.03
AACGATT202.3758002E-7225.09
ATGACTA202.3758002E-7225.0225
AAACAAT8550.0225.08
ACGCTGG100.0019019591225.01
Produced by FastQC (version 0.11.9)