FastQCFastQC Report
Wed 30 Nov 2022
4061-JW31trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW31trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90504
Sequences flagged as poor quality0
Sequence length236
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGCGCTCCCGAAGGCACTCCGAGCTTTCACTCAGATTCGCGACATGT1624617.9505878193229No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT63226.985326615398215No Hit
AGCACACCCTCGAAGGCGACTTCATTTCCGAAGCTTGCAGTGCTATGTCA62266.87925395562627No Hit
CAGCTAGTCAAGTAGAGTCTTCATCCCGACTATCATTCAGCTGTCTTATC47635.262750817643419No Hit
ACAGCACCCTCGAAGGCGACTTCATTTCCGAAGCTTGCAGCTGTATGTCA39404.353398744806859No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTAAC25692.8385485724387873No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC21962.426412092283214No Hit
ACAGCACCCTCGAAGGCGGCTTCATTTCCGAAGCTTGCAGCTGTATGTCA19052.104879342349509No Hit
ATAGCGGTCCCCGAAGGGAAAACTCTCTTTCAAGAGCGGTCCACCACAGT18822.0794661009458144No Hit
GTTCGCGCTCCCGAAGGCACCCTTCCCTTTCAAGAAGGTTCGCGACATGT16091.7778219747193496No Hit
CTCACTGTCCCCCGAAGGGGCCGACCTAATCTCTTAGGTATTCAGTGGAT11371.2562980641739592No Hit
CACCTCTGCCCCGAAGGGAAGGTCCCGTTACGGACCGGTCAGAGGGATGT10101.115972774683992No Hit
GTCCAGGCTCTCTTTCGAGCACTCCCAAATCTCTTCAGGATTCCTGGCAT7030.7767612481216299No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT6940.7668169362680103No Hit
GTTCGCGCTCCCGAAGGCACTCCAAGCTTTCACTCAGATTCGCGACATGT6220.6872624414390525No Hit
ATACATGTCCCGAAGGAAAGACGTATCTCTACGCCGGTCATGCACAGTTC5950.6574295058781933No Hit
ACAGGATCGCCGAAGCACACACCCTTTCGGATGCTTTACCCTGCAGTTTA4960.5480420754883762No Hit
GTCCAGGCTCCCTTTCGGGCACCAAGCCATCTCTGGCAAGTTCCTGGCAT4780.5281534517811368No Hit
GTGGACGCCAGCCGAACTGAAGGACCCGATCTCTCGAGCCCATACGTCCC3940.4353398744806859No Hit
GTGAACGCGTCCGAAGACAAGTCACCATCTCTGGTGATCATACGTTCCAT3730.4121364801555732No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTACC3660.4044020153805357No Hit
CAGCAGGGTTCCGGGCATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCAT3640.40219216830195353No Hit
CTCCGCGCCCCCGAAGGGGAACCCCCATCTCTGGAGGGTGCACGGGATGT3620.39998232122337135No Hit
CAGCAGGATTCCTGGCATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCAT3530.3900380093697516No Hit
CACCTCTGCCCCGAAGGGAGGATCCCGTTACGGACCGGTCAGAGGGATGT3470.38340846813400514No Hit
GTCCCAGTTCTCTTTCGAGCACTCCCGCCTCTCAGCAGGATTCCAGGCAT3380.3734641562803854No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCCGTTTCAGGAGGGTTCCGTAGAT3300.36462476796605675No Hit
CTCCTGGCTCCTTGCGGCACGTCCATGCATCTCTGCTAGACTTCCAGGGA3160.3491558384159816No Hit
GCAACTGTCCCGAAGGAAAGCACCCTTTCAGGTGCGGTCAATTGCATGTC3050.33700167948377974No Hit
CTGAAAATCTGGCAAGGTCATCAGCCTGGCCTTCATGTAACAGTCGCTCC2870.31711305577654025No Hit
CTCAGAGCTCCCGAAGGCACTCCGCTATCTCTAACAGATTCTCTGGATGT2760.3049588968443384No Hit
GTGGAAGCCAGCCGAACTGAAGGACCGCGTCTCTGCGGCCCATACTTCCC2500.27623088482277025No Hit
AGCACACCCTCGAAGGCGACTTCGTTTCCGAAGCTTGCAGTGCTATGTCA2440.26960134358702376No Hit
GTTCAGGCTCTCTTTCGAGCACTCCCAACTCTCATCGGGATTCCTGACAT2330.25744718465482186No Hit
GTGACACCCGGCCGAACCGAAGAGAGCGTCTCCGCTCCCGGCTGTGTCCA2250.24860779634049324No Hit
CTCCCGGTCGTATTGCTACGAAGACTGTATCTCTACAGCTGTCCGAGGAT2250.24860779634049324No Hit
CTCAGAGTTCCCGAAGGCACTCTACTATCTCTAACAGATTCTCTGGATGT2170.23976840802616461No Hit
GTGGGAGCCAGCCGAACTGAAGGACCCGATCTCTCAGGCCCATACTCCCC2170.23976840802616461No Hit
CACCTCTGCCCCGAAGGGAAGGACCCGTTACGGTCCGGTCAGAGGGATGT2080.22982409617254485No Hit
GTCCCGATTCCCTTTCGGGCACCCCCACCTCTCAGCAGGGTTCCGGGCAT2050.22650932555467163No Hit
GTCCAGGCTCTCTTTCGAGCACTCCCACCTCTCAGCAGGATTCCTGGCAT1870.20662070184743214No Hit
CGAAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAATCGTCGCTCC1850.20441085476885No Hit
GTCCGGTCCAGCCTAACTGAAGGACAATGTCTCCACTGTCCGCGACCGGC1780.19667638999381243No Hit
CTCCGATCCAGCCGAACTGACCCCGCTGCTTCCAGCAGGTACGATCGGGA1730.19115177229735703No Hit
CAGCAGGATTCCTGACATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCAT1670.18452223106161053No Hit
GTTCAGGTTCCCTTTCGGGCACGAATCCATCTCTGGAAACTTCCTGACAT1630.1801025369044462No Hit
CCAATTGTCCTTGTGAGAGGGCCAAAGTTTCCTAAGGCTTTCAATTGGGT1620.17899761336515513No Hit
GTTACGGCTCTCTTTCGAGCACCCCCACCTCTCAGCAGGGTTCCGTACAT1610.17789268982586406No Hit
ACACGGTGTCCGAAGACAGCCCCACTTTCATGGGGTTTACCGTGCGGTTC1580.1745779192079908No Hit
CTGGCAGTGTATTGCTACAAAGTGAGCTTTCACCCACGGTCTCCCAGCAT1520.16794837797224432No Hit
CTCAGAGTTCCCGAAGGCACTCTACGATCTCTCACAGATTCTCTGGATGT1500.16573853089366217No Hit
GTTCAGGCTCCCTTTCGGGCACTCCCAAATCTCTCCGGGATTCCTGACAT1330.1469548307257138No Hit
GTTCAGGTTCTCTTTCGAGCACTCCCACCTCTCAGCAGGATTCCTGACAT1310.14474498364713162No Hit
GTTCGCGCTCCCGAAGGCACTCCGCGCTTTCACTCAGATTCGCGACATGT1300.14364006010784053No Hit
AGCACACCCTCGAAGGCGACTTCATTTCCGAAGCTTGCAGCTGTATGTCA1290.14253513656854946No Hit
GTTCGCGTTCCCGAAGGCACTCTCTCATCTCTGAAAGATTCGCGACATGT1280.14143021302925837No Hit
ACAGCACCCTCGAAGGCGACTTCGTTTCCGAAGCTTGCAGCTGTATGTCA1280.14143021302925837No Hit
TGCGAGGGCACCATCACCGTCATCCAAGGCCTGATCCGCTTCGAGCCGAC1270.1403252894899673No Hit
CAGCTAGTCAAGTAGAGTCTTTAGCCCGACTATCATTCAGCTGTCTTAAC1240.13701051887209406No Hit
GTCCACGTCCCCGAAGGGAAGAAATCCATCTCTGGAAGTCGTCGTGGCAT1240.13701051887209406No Hit
ACAGCAGCTCCGAAGAGCTATAGTATCTCTACCATATTCCACTGTATGTC1230.13590559533280297No Hit
CCTACACCCTCGAAGGCTTCCTATCTTTCGATAGGCGTGCAGTAGGATTT1190.13148590117563866No Hit
GCAAGCTCCCCGAAGGGTCGATCCACTTTCGCTTCTCTACCACTTGCATG1190.13148590117563866No Hit
GTTCAGGCTCTCTTTCGAGCACTCCCACCTCTCAGCAGGATTCCTGACAT1160.12817113055776538No Hit
GTTCGCGCTCCCGAAGGCACTCCGAGCTTTCACTCCGATTCGCGACATGT1150.12706620701847432No Hit
ACCAGCTTCCGGATTGGATACCGTTCGTTCCCGTTTCCGGGTCCTACCTG1140.12596128347918326No Hit
GTCCAGGTTCCCTTTCGGGCACCAATCCATCTCTGGAAAGTTCCTGGCAT1120.12375143640060107No Hit
CAGCAGGGTTCCGTACATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCAT1110.12264651286131No Hit
GCAAGTTTCACCCCGAAGGGCAGCCTACTCTGTTTCCAGATGGGCATCCA1100.1215415893220189No Hit
GCTACGGTTCTTGCGAAAAGGCACATCTCTGTACCGGTCCCTAGCAGTTC1090.12043666578272784No Hit
GTGGACGCGTCCGAAGACAAGATACCATCTCTGGTAAACATACGTCCCAT1060.11712189516485459No Hit
CACCGGTCCAGCCGAACTGAAGGACAATGTCTCCACTGTCCGCGACCGGG1060.11712189516485459No Hit
GTTAACGTTCTCTTTCGAGCACCAATCCATCTCTGGAAAGTTCGTTACAT1040.11491204808627242No Hit
CAGCTAGTCAAGTAGAGTCTTCAACCCGACTATCATTCAGCTGTCTTAAC1020.11270220100769027No Hit
ACAGCACCCTCGAAGGCGACTTCATTTCCGAAGCTTGCAGTGCTATGTCA1010.11159727746839919No Hit
CTTGGGGTTCATCTTACGAAGCACTCCCGACTTTCATCAGGATTCCCCAG1000.1104923539291081No Hit
GTTCAGATTCCCTTTCGGGCACGATCCCATCTCTGGAAACTTTCTGACAT980.10828250685052593No Hit
CAGCTCGTCAAGTAGAGTCTTCATCCCGACTATCATTCAGCTGTCTTATC970.10717758331123486No Hit
GTTCCGATTCCCTTTCGGGCACCCCCACCTCTCAGCAGGGTTCCGGACAT930.10275788915407053No Hit
CTCTGCGCTTCCCGAAGGACACTCCCTCATCTCTGAAGGATTCGCAGGAT920.10165296561477945No Hit
GTTCGCGCTCCCGAAGGCACCCTTCTCTTTCAAGAAGGTTCGCGACATGT910.10054804207548838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAA100.0017808523230.000029
GATCGCG100.0017808523230.000021
CGAAAAA202.1292362E-7230.000021
ACGTTGT100.0017808523230.000023
CGGCCTA100.0017808523230.000025
GTGGGCG100.0017808523230.000021
TTATGGC100.0017808523230.00002230
TAGCCAG100.0017808523230.000025
GTTGTAC202.1292362E-7230.000025
ACGGCCT100.0017808523230.000024
ACGTGCC100.0017808523230.000023
ACCAGCT100.0017808523230.000021
GAAAAAT202.1292362E-7230.000022
TCCAAAG100.0017808523230.000029
CAAGCAA100.0017808523230.000029
GACATCC100.0017808523230.000024
GCGTGGG202.1292362E-7230.00002230
AGCTCGG100.0017808523230.000022
GCAGCTC202.1292362E-7230.000024
TTTTGTG100.0017808523230.000021
Produced by FastQC (version 0.11.9)