FastQCFastQC Report
Wed 30 Nov 2022
4061-JW32trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW32trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147458
Sequences flagged as poor quality0
Sequence length231
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA7316849.61955268618861No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA1947013.203759714630605No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA45033.053750898560946No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGTGCGGTGCGACCGCATCTG34372.3308331863988387No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA24841.6845474643627338No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG14410.977227413907689No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG13760.9331470656051215No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG8360.5669410950914837No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG4440.30110268686676883No Hit
TAGCCGTAAACTATGGATACTGGATCTTGCATATATTTGCTTGGTCGAAG4300.29160845800160046No Hit
TAGCCGTAAACGATGGGCATTGGGTGCTGGCTCGCAAGGGTCGGTGTCGT3750.25430970174558176No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG3460.23464308481059015No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA3450.23396492560593526No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG3210.217689104694218No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA3210.217689104694218No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC3100.21022935344301427No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2930.19870064696388126No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG2670.18106850764285423No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA2620.17767771161957982No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG2560.1736087563916505No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCCTGAAGGGCTTGGTCGTAGC2560.1736087563916505No Hit
ACGCCGTAAACGATGAATACTAGGTGTAGGGGGTATCGACCCCCCCTGTG2560.1736087563916505No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2370.16072373150320768No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG2200.14919502502407464No Hit
TAGCAGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG2180.1478387066147649No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT2100.1424134329775258No Hit
ACGCCCTAAACAATGTTCACTTGGTGTCGGCCGCTTATACTGGTCGGTGC2090.14173527377287093No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1960.13291920411235741No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA1930.13088472649839278No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA1910.129528408089083No Hit
AGGGTGTAAACGCTGCTCGCTTGATGTTAGTCGGGCTCCGAGCCCAACTA1810.12274681604253414No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG1810.12274681604253414No Hit
GGAAAGGCGTTCATGGCGCAGATGTCCGAGACGTTGACGCTGATCGCCTT1690.11460890558667554No Hit
GGAAAAGCGTTCATGGCGCAGATGTCCGAGACGTTGACGCTGATCGCCTT1620.10986179115409134No Hit
TAGCCGTAAACGATGTGAACTTGGCGTCGGTGGGTTAAACTCCATCGGTG1560.10579283592616204No Hit
TAGCAGTAAACGATGTAGACTTGGTGTCGGAGGTGTCAAAACCTTCGGTA1560.10579283592616204No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTGGTCG1550.10511467672150714No Hit
ACGCCCTAAACAATGTTCACTTGGTGTCGGCCGCTCATACTGGTCGGTGC1540.10443651751685225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATC152.130649E-5224.99254225
ACACGAT152.130649E-5224.992548
GGTCGTC100.0019033585224.99254225
GGCGTTC100.0019033585224.992546
CTATAAA100.0019033585224.992544
GCTATAA100.0019033585224.992543
AAAAGAT350.0224.992548
TTGTCAA100.0019033585224.99254225
CACGATG152.130649E-5224.992549
GCCATAA100.0019033585224.992543
CGTTCAT303.092282E-11224.992548
TGGCCGT650.0224.992541
CTGAATC1650.0224.99254225
CGCCTTA303.092282E-11224.992542
AACGCTG1100.0224.992549
GCCCTAA3300.0224.992543
CCATAAA152.130649E-5224.992544
CGAATTT303.092282E-11224.99254225
AAACACT550.0224.992548
CGTACAC152.130649E-5224.992545
Produced by FastQC (version 0.11.9)