FastQCFastQC Report
Wed 30 Nov 2022
4061-JW32trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW32trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147458
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA5109934.65325719865996No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA2387616.19172917034003No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA81945.556836522942126No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA77105.228607467889161No Hit
CAGCGAATTCGGTAAAGTCTTTAGCTTGACCTTCATATTGCTGTCTGACC32272.1884197534213135No Hit
TACAGCTCCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA18861.2790082599791128No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC15431.0463996527824873No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA10600.7188487569341779No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC10200.6917223887479825No Hit
TACAGTTCCCCGAAGGACCTCCTCTTTCAAGGAGTTTAACTGCAATATCA7920.5371020900866688No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA7900.5357457716773589No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC5100.34586119437399127No Hit
TACGACTCGCCGAAGCACCTCATCTTTCAATGAGTTTAGTCGCAATATCA5050.3424703983507168No Hit
GTATGCTCCCGATTTATACGGGTCGTTGCCCTTTCAGGTTCCTACTACAT3720.2522752241316172No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC3370.22853965196869616No Hit
ACAGGTTTCTCCAAAGGAGAATCACTATCCCTTTCAGGTAGCTATGCCTG2980.20209144298715567No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC2830.19191905491733238No Hit
ACTAGCTCTCTACCCGAAGGCAGAGTCGTCCCCCTTTCAGGTTCCTACCA2210.14987318422872953No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG2040.1383444777495965No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC1850.12545945286115368No Hit
CTCTCTGTCCCCGAAGGGAAAACGCAATCTCTTGCGTCGTCAGAGGATGT1830.12410313445184393No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGTGAGTTTAGCTGCAATATCA1720.11664338320064019No Hit
GCAGCAAGGCGATCAGCGTCAACGTCTCGGACATCTGCGCCATGAACGCC1680.11393074638202064No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATCCCTACCACCTGTATG1620.10986179115409134No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGCTTAGCTGCAATATCA1580.10714915433547179No Hit
GCAGCAAGGCGATCAGCGTCAACGTCTCGGACATCTGCGCCATGAACGCT1580.10714915433547179No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC1520.10308019910754249No Hit
CTTCCTGCCCCGAAGGGCTTCCCCGGTTAAGGGTAATTCAGGAGATGTCA1500.10172388069823272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCGA650.0230.071249
ATCAGGT252.3410394E-9230.071247
TAAGTAA252.3410394E-9230.071249
GAATTCG4100.0230.071245
TTCGAAA252.3410394E-9230.071249
AAATCAG350.0230.071235
AATCAGG450.0230.071236
GAGCTGT100.0017803349230.071235
GTCTGGT400.0230.071237
AGATAAC100.0017803349230.071236
CTATATT100.0017803349230.071239
GCCATTG100.0017803349230.071237
CAGATAA100.0017803349230.071235
CAAGGCG302.5465852E-11230.071235
GTGTATT151.9491126E-5230.071237
TGTTAAG202.130455E-7230.071236
AATTCGG4050.0230.071236
GCCTATA100.0017803349230.071237
TCCCGAG151.9491126E-5230.071238
GGCGCAT151.9491126E-5230.071236
Produced by FastQC (version 0.11.9)