FastQCFastQC Report
Wed 30 Nov 2022
4061-JW33trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW33trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68956
Sequences flagged as poor quality0
Sequence length231
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA3061644.399327107140785No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA56268.158825917976682No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA42586.174952143395789No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG8201.1891641046464412No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG6840.991936887290446No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA5840.8469168745286849No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG5050.7323510644468937No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG4030.5844306514298973No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG3670.5322234468356634No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG2730.39590463483960786No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA2550.36980103254249086No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG2490.3610998317767852No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG2350.34079702999013867No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2240.32484482858634495No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG2230.3233946284587273No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT2180.31614362782063926No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA1950.2827890248854342No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGTGCGGTGCGACCGCATCTG1940.2813388247578166No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1910.2769882243749638No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC1760.2552352224606996No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT1700.24653402169499392No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA1690.2450838215673763No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA1580.2291316201635826No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG1480.21462961888740645No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG1370.19867741748361276No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA1340.1943268171007599No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG1280.18562561633505426No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG1270.18417541620743663No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG1260.182725216079819No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG1230.17837461569696617No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1150.1667730146760253No Hit
ACGCCGGAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA1100.15952201403793723No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA1050.1522710133998492No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA1050.1522710133998492No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCCTGAAGGGCTTGGTCGTAGC1020.14792041301699635No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTGGTCG990.1435698126341435No Hit
TAGCAGTAAACGATGTAGACTTGGTGTCGGAGGTGTCAAAACCTTCGGTA960.13921921225129066No Hit
AGGGTGTAAACGCTGCTCGCTTGATGTTAGTCGGGCTCCGAGCCCAACTA950.13776901212367307No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG910.13196821161320263No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCATCAATTTGCTTGGTCGTAG850.12326701084749696No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG840.12181681071987933No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG820.11891641046464412No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG820.11891641046464412No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGTGGGCGCCCA780.11311560995417368No Hit
ACGCCGTAAACGATGAGAACTAGGTGTTGCGGGTATTGACCCCTGCAGTG760.11021520969893846No Hit
ACGCTGTAAACGTTGTGTACTAGGTGTTAGTGACTTGTTCACTAGTGCCG720.104414409188468No Hit
ACGCCGTAAACGATGGATACTAGGTGTGGGAGGTATCGACCCCTTCCGTG710.10296420906085038No Hit
ACACTGTAAACGATGTACACTAGGTGCTGGGGCGGATGCTTCAGTGCCGT700.10151400893323279No Hit
ACACGGTAAACGATGAATGCTAGGTGTAGGGGGTATCGACCCCTTCTGTG690.10006380880561516No Hit
TAGCAGTAAACGATGTAGACTTGGCGTCGGTGGGTTAAAAACCATCGGTG690.10006380880561516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAATG152.125467E-5225.000029
AAACTAT1000.0225.000028
TAACGAT152.125467E-5225.000028
CACTGTA252.6593625E-9225.000022
TAGCAGT350.0225.000021
CTGAATC700.0225.00002225
CCGTAAC152.125467E-5225.000024
CAAGGGC302.910383E-11225.00002225
CGGTAAA302.910383E-11225.000024
AGCCCTA350.0225.000022
GTAGACG100.0019009794225.06
TGCATCC100.0019009794225.0225
AGCTTTA202.3717257E-7225.0225
AACCGAT100.0019009794225.08
TAAACAA202.3717257E-7225.07
AAACAAT202.3717257E-7225.08
CACCGTT100.0019009794225.02
ACCGATG100.0019009794225.09
GCAATAG100.0019009794225.0225
CGGCGTA100.0019009794225.02
Produced by FastQC (version 0.11.9)