FastQCFastQC Report
Wed 30 Nov 2022
4061-JW33trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW33trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68956
Sequences flagged as poor quality0
Sequence length236
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA2477335.925807761471084No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA923013.38534717791055No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA44366.433087766111724No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA22143.2107430825453913No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC8311.205116306050235No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA4340.6293868553860432No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC3420.495968443645223No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC3320.48146644236904695No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT2150.3117930274377864No Hit
TACGACTCGCCGAAGCACCTCATCTTTCAATGAGTTTAGTCGCAATATCA1920.2784384245025813No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC1700.24653402169499392No Hit
CAGCGAATTCGGTAAAGTCTTTAGCTTGACCTTCATATTGCTGTCTGACC1560.22623121990834735No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG1430.20737861824931839No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC1400.20302801786646557No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA1270.18417541620743663No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC1260.182725216079819No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA1250.1812750159522014No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA1160.1682232148036429No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA1060.1537212135274668No Hit
CTTCCTGCCCCGAAGGGCTTCCCCGGTTAAGGGTAATTCAGGAGATGTCA1010.14647021288937873No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGTGAGTTTAGCTGCAATATCA950.13776901212367307No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA890.1290678113579674No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC870.1261674111027322No Hit
TACAGCGTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA860.12471721097511457No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT840.12181681071987933No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC790.1145658100817913No Hit
TACACCTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA790.1145658100817913No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT780.11311560995417368No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT770.11166540982655608No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT760.11021520969893846No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG750.10876500957132085No Hit
GAAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCAACATG740.10731480944370322No Hit
ACTGGAGTCCCCGAAGGGAAGGCCTCATCTCTGAGGCTGCCCCCAGCAGT740.10731480944370322No Hit
CAGCTAGTCAAGCAGAGTCTTCAACCCGGCTATCATTCAGCTGTCTTATC730.10586460931608561No Hit
TACAGCTTCCCGAAGGACATCATCTTTCAATGAGTTTAGCTGCAATATCA720.104414409188468No Hit
GAAGGCTCCCTTACGGGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG710.10296420906085038No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATCTCTACCACCTGTATG700.10151400893323279No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTG100.0017799351230.000024
TTAGGTA100.0017799351230.000028
TTGGACT100.0017799351230.00002230
GTCTGGT100.0017799351230.000027
TGCCAAT100.0017799351230.000026
CGAAAAA100.0017799351230.000021
TTTCGTG100.0017799351230.000021
CATTGTC100.0017799351230.000024
CAGCAGC100.0017799351230.000022
ATTCGGT202.12558E-7230.000027
AAGCGTC100.0017799351230.000023
AGGCGTA100.0017799351230.000025
GTGGCTA100.0017799351230.00002230
TGACGAC100.0017799351230.000022
GCTGACG100.0017799351230.00002230
CCAGATT100.0017799351230.000023
GTGTGTA100.0017799351230.000025
GTTACTG100.0017799351230.00002230
ACAGGCT100.0017799351230.000021
GTTGTAC100.0017799351230.000025
Produced by FastQC (version 0.11.9)