FastQCFastQC Report
Wed 30 Nov 2022
4061-JW34trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW34trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149611
Sequences flagged as poor quality0
Sequence length231
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA5796238.74180374437709No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA135379.048131487657992No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA78535.248945598919866No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG33542.2418137703778465No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA25001.6710001269960097No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG18661.2472344947898217No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG17251.1529900876272468No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG11090.7412556563354299No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG10960.7325664556750506No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT7640.5106576388099806No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG6800.4545120345429146No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG6650.44448603378093854No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG6420.4291128326125753No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT6130.40972923113942156No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC6070.4057188308346312No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG5440.3636096276343317No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA5180.34623122631357317No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG4900.3275160248912179No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG4800.3208320243832339No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA4660.3114744236720562No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG4310.2880804218941121No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG4310.2880804218941121No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG4230.28273322148772484No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG4160.278054421132136No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG3890.26000761976057907No Hit
ATGCCGTAAACGATGCAGACTAGGTATCGCGGGGGTAAAACCCGGCGGCG3850.2573340195573855No Hit
ACACTGTAAACGATGTGCACTAGGTGCTGGGGCGGATGCTTCAGTGCCGG3650.24396601854141742No Hit
ACGCCGTAAACGATGAGCACTAAGTGTCGGGCGAATGCTCGGCGCTGCAG3570.2386188181350302No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG3400.2272560172714573No Hit
ACGCTGTAAACACTCCCTGTTAGATATTTGGTAGTTCATTGAAATTTTTT3360.22458241706826368No Hit
ACGCCGTAAACGATGAGAACTAGGTGTTGCGGGTATTGACCCCTGCAGTG3010.20118841529031956No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG2910.19450441478233552No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA2790.18648361417275466No Hit
ACGCTGTAAACGTTGTGTACTAGGTGTTAGTGACTTGTTCACTAGTGCCG2760.18447841402035947No Hit
TAGCTGTAAACGATGTGAACTTGGTGTCGGTGGGGTAAAAGCCATCGGTG2680.17913121361397222No Hit
ACACTGTAAACGATGTACACTAGGTGCTGGGGCGGATGCTTCAGTGCCGT2630.1757892133599802No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG2590.17311561315678659No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTGGTCG2560.1711104130043914No Hit
ACGCTGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA2550.170442012953593No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA2510.16776841275039936No Hit
ACGCTGTAAACGATGGGTACTGGGTTTCGGGGGTCTCAACCCTCTCGGAG2490.16643161264880257No Hit
ACGCCGTAAACGATGGGCACTTGGTATCGCGGGTATTGACCCCTGCGGTG2490.16643161264880257No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC2490.16643161264880257No Hit
ACGCTGTAAACGATGTTCACTAGGTGTTGGGGGTATTGACCCTCTCAGTG2450.16375801244560895No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGGGTAAAATCCATCGGTG2420.16175281229321373No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG2300.15373201168363287No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA2260.15105841148043928No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGTGGGTTTAAATCCATCGGTG2220.14838481127724565No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA2200.14704801117564886No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG2130.14236921082006002No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2110.14103241071846323No Hit
ACGCCGTAAACGATGAACACTAGTTGTGTGCGATACACAGTACGCGACTG2090.13969561061686642No Hit
GTGCCGTAAACGATGTGGACTAGGTGTCGGCGACGCGAGTCGGCGGTGCC2060.1376904104644712No Hit
TAGCAGTAAACGATGTAGACTTGGTGTCGGAGGTGTCAAAACCTTCGGTA2010.13434841021047916No Hit
ACGCGGTAAACGATGTGCACTAGATGTCGGGGGTATTGATCCCTTCGGTG1920.12833280975329353No Hit
ACGCCGTAAACGATGATTACTCGTTGTTGGCGATATATTGTCAGCGACTG1910.12766440970249515No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCCTGAAGGGCTTGGTCGTAGC1900.12699600965169672No Hit
ACGCCGTAAACGATGTTCACTAGGTGTTGGGGGTATTGACCCCCTCAGTG1820.1216488092453095No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATCAATTTGCTTGGTCGTAG1790.11964360909291429No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA1790.11964360909291429No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA1770.11830680899131749No Hit
ATGCTGTAAACGATGTGAACTAGGTGTTGCGCCGTCAAGGGGCAGTGCCG1700.11362800863572865No Hit
TGGCAGTAAACGATGTGAACTTGGCGTCGGCGGGATAAAACCTGTCGGTG1690.11295960858493026No Hit
TAGCAGTAAACGATGTAGACTTGGCGTCGGTGGGGTAAAATCCGTCGGTG1690.11295960858493026No Hit
ACGCCCTAAACGATGGGTACTTGGTGTCGCGGGTATCGACTCCCGCGGTG1660.11095440843253504No Hit
CAGCTGTAAACGATGCAAACTAGGTGTTTGAACGGCCACGTGCCGTTCTA1660.11095440843253504No Hit
CAGCTGTAAACGATGCTAGCTAGGTGTTGGGTCGGCCACGCGCCGCCCCA1650.11028600838173665No Hit
ACGCTGTAAACGATGAACACTTGGTGTGGAGGGAGTTGACCCCTTCCGTG1650.11028600838173665No Hit
ACGCTGTAAACGATGAATACTAGGTGTTGGGTTTTAAACTCAGTGCCGCA1640.10961760833093823No Hit
ACACGGTAAACGATGAATGCTAGGTGTAGGGGGTATCGACCCCTTCTGTG1630.10894920828013983No Hit
ACGCCCTAAACGATGGGCACTAGGTGTGGGAGGTATCGACCCCTTCCGTG1630.10894920828013983No Hit
TAGCAGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG1610.107612408178543No Hit
CAGCCGTAAACGATGAACACTAGGTGTGGGGGGTATCGACCCCTTCCGTG1600.10694400812774463No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGTGGGCGCCCA1560.10427040792455101No Hit
CAGCTGTAAACTATGCTCGCTAGGTGTCAGGGACGGTGCGACCGTTTCTG1550.1036020078737526No Hit
TAGCAGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT1530.1022652077721558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATAAA350.0225.000024
CATAAAC350.0225.000025
AACAATG202.3809662E-7225.09
AACAATC100.0019031973225.09
TTTACTA152.1304295E-5225.0225
CAACGAT303.092282E-11225.08
ACCATAA100.0019031973225.03
CCCTAAA4550.0225.04
AACTTTG152.1304295E-5225.09
AACCATG152.1304295E-5225.09
CTATAAA100.0019031973225.04
GCTATAA100.0019031973225.03
ACCGATG252.6757334E-9225.09
TAAACAA303.092282E-11225.07
CACGATG100.0019031973225.09
CACTGTC100.0019031973225.02
ACCCTAA152.1304295E-5225.03
AGACGAT152.1304295E-5225.08
CCATCAA100.0019031973225.04
GCCCTAA4400.0225.03
Produced by FastQC (version 0.11.9)