FastQCFastQC Report
Wed 30 Nov 2022
4061-JW34trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW34trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149611
Sequences flagged as poor quality0
Sequence length236
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA3177721.23974841422088No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA1997213.349285814545722No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA1870612.503091350234943No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA95976.414635287512283No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC23801.5907921209002012No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC23401.564056118868265No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT10750.7185300546082841No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC7370.49261083743842365No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC6790.4538436344921162No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA6600.4411440335269466No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC6080.4063872308854295No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT6080.4063872308854295No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC5480.3662832278375253No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA5090.34021562585638754No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA4910.3281844249420163No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA4710.31481642392604825No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT4670.3121428237228546No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA4440.2967696225544913No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA4360.2914224221481041No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG4170.2787228211829344No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT4000.26736002031936157No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG3510.23460841783023975No Hit
GTAGCACCTTCGAAAGCTGAATCCATCTCTGGAAACGTGCAGCTACAGTT3260.21789841656027967No Hit
CTCCATTGTCCCGAAGGAAAGGCTTATCTCTAAGCCGGTCAATGGGATGT3200.21388801625548926No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT3160.21121441605229563No Hit
TACACCTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA3060.2045304155443116No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA2930.19584121488393233No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT2830.1891572143759483No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC2780.18581521412195628No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG2610.1744524132583834No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC2570.1717788130551898No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC2510.16776841275039936No Hit
ACAGCGTCGTATTGCTACGCTAGATCATCTCTGACCTATCACACTGTATG2500.16710001269960095No Hit
GGCCGCGCCTATTGCTAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG2470.16509481254720576No Hit
ACAAGTGCCATTGCTGGAGGGGTACTTTCATACCCTGTCACTTGCCTTTC2460.16442641249640735No Hit
ATACCTGTCCGAAGAAGGGTGTATCTCTACACCTGTCAGGTACAGTTCAA2400.16041601219161694No Hit
CAGCTAGTCAAGCAGAGTCTTCAACCCGGCTATCATTCAGCTGTCTTATC2400.16041601219161694No Hit
GTGGACTAACCCTGCTTTCACAGGGCTGACCCCGCCCGAAGGCGGAGCTC2380.15907921209002013No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA2210.14771641122644724No Hit
ACTGGAGTCCCCGAAGGGAAGGCCTCATCTCTGAGGCTGCCCCCAGCAGT2150.14370601092165683No Hit
GTGACAGCTCCTTGCGGAGAGGGTGGCTTTCACCACCTGTCTGCCCCTCT2140.14303761087085842No Hit
ATAGGCTCCCCGAAGGGTCGGTCGCATCTCTGCTCCCTACCACCTATATG2120.1417008107692616No Hit
ATGACGACCCCGAAGGGAGGGAGTGTTTCCACTCCTGTCCGTCACGGTTC2070.1383588105152696No Hit
GCAGGGCCCTCTCGGACACGACGTTTCCGCCGCTTTACCCTGCATGTCAA2070.1383588105152696No Hit
ACAGGCTCCCCGAAGGGTCGGTCACCTCTCGGATCCCTACTACCTGTATG2030.13568521031207598No Hit
TACGACTCGCCGAAGCACCTCATCTTTCAATGAGTTTAGTCGCAATATCA2030.13568521031207598No Hit
CAGCTCGTCTAGCAAGACCTTCAATCTGGCCTTCACTCTGCTGTCGATCC2000.13368001015968078No Hit
ACTGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCAGTATGTC1910.12766440970249515No Hit
CGGAAAATCAGGTAAAGTCATCAGCTTGACCTTCATATATCCGTCGCTCC1880.12565920955009993No Hit
CACCGGTCCAGCCGAACTGACCCACCTGTTTCCAGGCAGTACGACCGGGA1850.12365400939770473No Hit
ATAGGCTCCCCGAAGGGTCGGTCACCTCTCGGCTTCCTACCACCTATATG1830.1223172092961079No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC1830.1223172092961079No Hit
CACCGATCCAGCCGAGCTGACCCGCCTGTTTCCAGGCAGTACGATCGGGA1810.12098040919451108No Hit
ACAGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCTGTATGTC1800.12031200914371269No Hit
GAAGGCTCCCTTACGGGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG1790.11964360909291429No Hit
CTTACGGCTCCCCGAAGGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT1680.11229120853413184No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT1660.11095440843253504No Hit
CAGCGATTCTGGTAAAGTCTTTAGCTTGACCTACATATTGCTGTCGCCCC1650.11028600838173665No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATCTCTACCACCTGTATG1610.107612408178543No Hit
GCAAGCTCCCCGAAGGGTCGGTCTGCTTTCGCTTCCCTACCACTTGCATG1590.1062756080769462No Hit
ACGAAGGCGTATTGCTACGCTGCGATATCGCTACCGCATTCCTCCGTATG1580.10560720802614781No Hit
GAAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCAACATG1550.1036020078737526No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATCCCTACCACCTGTATG1520.10159680772135737No Hit
GAGGGCTCCCCGAAGGGTCGGTCTGCTTTCGCTTCCCTACCACCCACATG1510.10092840767055898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGTT100.001782012230.000021
GGAGCTG100.001782012230.000024
TTGGACT202.1338565E-7230.00002230
CAAAAGC100.001782012230.000022
ATGGCTC202.1338565E-7230.000022
CGAAAAA100.001782012230.000021
TGGACGC100.001782012230.000022
GGACTAA202.1338565E-7230.000023
TAGCGCT100.001782012230.000023
AAAAGCT100.001782012230.000023
ATAGCAC100.001782012230.000021
GCAAAAG100.001782012230.000021
CAATTGC202.1338565E-7230.000029
CGATTCT100.001782012230.000024
TCCTATA202.1338565E-7230.000027
AAAACTC100.001782012230.000022
CCCGTAG100.001782012230.000029
ACAGGCC100.001782012230.000023
GGTATTG100.001782012230.000028
GAAGTTT100.001782012230.000022
Produced by FastQC (version 0.11.9)