FastQCFastQC Report
Wed 30 Nov 2022
4061-JW36trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW36trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85762
Sequences flagged as poor quality0
Sequence length231
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA3195637.26125789976913No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA58076.771064107646743No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG18952.209603320818078No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA16181.8866164501760687No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG15221.7746787621557334No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG13851.6149343532100464No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA10271.1975000583008792No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG6400.7462512534689023No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG6010.7007765677106411No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG5880.685618339124554No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG5790.6751241808726476No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT5660.6599659522865605No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG4260.49672349059023807No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT4210.4908934026725123No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG4080.4757351740864252No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG3980.46407499825097365No Hit
ACGCCGTAAACGATGAGCACTAAGTGTCGGGCGAATGCTCGGCGCTGCAG3890.45358083999906723No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG3840.44775075208134135No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA3750.43725659382943494No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG3240.3777896970686318No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCCTGAAGGGCTTGGTCGTAGC2640.3078286420559222No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC2530.2950024486369255No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA2450.28567430796856413No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG2380.27751218488374807No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2350.27401413213311254No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC2310.2693500617989319No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA2270.2646859914647513No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA2260.2635199738812061No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA2200.2565238683799352No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTGGTCG2190.25535785079639No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA2060.24019962221030292No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG2050.23903360462675777No Hit
ACACTGTAAACGATGTACACTAGGTGCTGGGGCGGATGCTTCAGTGCCGT2050.23903360462675777No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG2020.23553555187612227No Hit
TAGCAGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT1990.23203749912548682No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG1860.21687927053939973No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG1750.204053077120403No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG1730.20172104195331267No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA1680.19589095403558685No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG1630.19006086611786105No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA1560.18189874303304493No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCATCGAG1510.17606865511531913No Hit
ACGCCGTAAACGATGGGCACTTGGTATCGCGGGTATTGACCCCTGCGGTG1500.17490263753177399No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG1480.17257060236468366No Hit
TAGCTGTAAACGATGTGAACTTGGTGTCGGTGGGGTAAAAGCCATCGGTG1330.15508033861150625No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG1300.15158228586087077No Hit
ACGCCGTAAACGATGAGCACTAGGTGTCGGGGTCGCAAGACTTCGGTGCC1220.1422541451925095No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGTGGGCGCCCA1220.1422541451925095No Hit
ACGCTGTAAACGTTGTGTACTAGGTGTTAGTGACTTGTTCACTAGTGCCG1220.1422541451925095No Hit
ACGCTGTAAACGATGGGTACTGGGTTTCGGGGGTCTCAACCCTCTCGGAG1210.14108812760896433No Hit
ACGCCGTAAACGATGAGTGCTAAGTGTTGGAGGGTTTCCACCCTTCAGTG1200.13992211002541918No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCCTGAAAGGCTTGGTCGTAGC1180.13759007485832886No Hit
CAGCCGTAAACGATGTGCATTAGGTGTATCGGTGACCACGAGTCACCGAG1100.1282619341899676No Hit
ATGCCGTAAACGATGCAGACTAGGTATCGCGGGGGTAAAACCCGGCGGCG1060.12359786385578696No Hit
ACGCCGTAAACGATGAATGCCAGCCGTTAGTGGGTTTACTCACTAGTGGC1060.12359786385578696No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGTGGGTTTAAATCCATCGGTG1040.12126582868869663No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTGTCAGTGACCACGTGTCACTGAG1020.1189337935216063No Hit
ACGCTGTAAACGATGTTCACTAGGTGTTGGGGGTATTGACCCTCTCAGTG1000.11660175835451597No Hit
ACGCCGTAAACGATGAGAACTAGGTGTTGCGGGTATTGACCCCTGCAGTG970.1131037056038805No Hit
TAGCCCTAAACGATGAATACTAGGTGTTGGAGGATTCACTCCTTCAGCGT970.1131037056038805No Hit
ACACGGTAAACGATGAATGCTAGGTGTAGGGGGTATCGACCCCTTCTGTG960.11193768802033534No Hit
CAGCTGTAAACGATGCAAACTAGGTGTTTGAACGGCCACGTGCCGTTCTA940.10960565285324503No Hit
AGGGTGTAAACGCTGCTCGCTTGATGTTAGTCGGGCTCCGAGCCCAACTA920.1072736176861547No Hit
ACGCCGTAAACGATGAGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG920.1072736176861547No Hit
CAGCTGTAAACGATGCTAGCTAGGTGTTGGGTCGGCCACGCGCCGCCCCA910.10610760010260954No Hit
ACGCCCTAAACGATGGGTACTTGGTGTCGCGGGTATCGACTCCCGCGGTG900.10494158251906438No Hit
ACGCCGTAAACGATGAGAACTAAGTGTTGGCGCAAGTCAGTGCTGAAGTT890.10377556493551922No Hit
ACACTGTAAACGATGTGCACTAGGTGCTGGGGCGGATGCTTCAGTGCCGG870.10144352976842891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT252.6648195E-9225.000021
ACGCCAT152.1272694E-5225.000021
CACGGTA252.6648195E-9225.000022
TAGCCCT252.6648195E-9225.000021
CATAAAC303.092282E-11225.000025
AGAGGGC303.092282E-11225.00002225
ATCTGTA252.6648195E-9225.00002225
CGCCATA152.1272694E-5225.000022
AACACTG152.1272694E-5225.000029
AACGTTG1300.0225.09
CAGAATC100.0019017853225.0225
AAACTAT1600.0225.08
TTGGCAG100.0019017853225.0225
CTGTATC100.0019017853225.0225
GGTCGTC100.0019017853225.0225
CAACGAT100.0019017853225.08
CCGGATC100.0019017853225.0225
ACCGATG100.0019017853225.09
TAACGAT202.3750727E-7225.08
TGGCCGT350.0225.01
Produced by FastQC (version 0.11.9)