FastQCFastQC Report
Wed 30 Nov 2022
4061-JW36trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW36trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85762
Sequences flagged as poor quality0
Sequence length236
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA2815332.82689302954688No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA75718.827919125020406No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA64647.537137660035913No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC21562.513933910123365No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA15531.8108253072456333No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC15281.7816748676570042No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC10521.2266504978895083No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA7760.904829644831044No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG4460.5200438422611413No Hit
CTCCATTGTCCCGAAGGAAAGGCTTATCTCTAAGCCGGTCAATGGGATGT4330.5048856136750542No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC4300.5013875609244187No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA4260.49672349059023807No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC4080.4757351740864252No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA3940.459410927916793No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT3780.44075464658007046No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC3690.43026048832816394No Hit
CTTCCTGCCCCGAAGGGCTTCCCCGGTTAAGGGTAATTCAGGAGATGTCA3270.3812877498192673No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT2630.3066626244723771No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA2440.284508290385019No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC2230.26002192113057065No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA2220.2588559035470255No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC2190.25535785079639No Hit
CAGCTAGTCAAGCAGAGTCTTCAACCCGGCTATCATTCAGCTGTCTTATC2120.24719572771157391No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC1880.21921130570649006No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG1800.20988316503812876No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA1610.18772883095077075No Hit
CAGCTAGTCAAGTAGAGTCTTCATCCCGACTATCATTCAGCTGTCTTATC1590.18539679578368043No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGTTTCCTACCACCTGTATG1590.18539679578368043No Hit
ACAGCGTCGTATTGCTACGCTAGATCATCTCTGACCTATCACACTGTATG1590.18539679578368043No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT1540.17956670786595463No Hit
ATGACGACCCCGAAGGGAGGGAGTGTTTCCACTCCTGTCCGTCACGGTTC1520.1772346726988643No Hit
GCAGGTTCCTTCAAAGAAGGATTGCGATCCCTTTCAGGTAGCTATGCCTA1450.16907254961404816No Hit
GCTCCAGGCTCCGAAGAGAAGGTCACATCTCTGCGACCGGTCCTGGACAT1430.16674051444695784No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG1360.15857839136214175No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTAAC1350.15741237377859657No Hit
GCAGGGCCCTCTCGGACACGACGTTTCCGCCGCTTTACCCTGCATGTCAA1350.15741237377859657No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATACAGCTGTCTTATC1280.14925025069378045No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA1280.14925025069378045No Hit
ACAGGCTCCCCGAAGGGTCGGTCACCTCTCGGATCCCTACTACCTGTATG1270.1480842331102353No Hit
GGCCGCGCCTATTGCTAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG1270.1480842331102353No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT1270.1480842331102353No Hit
GCAGGTTCCTTCAAAGAAGGATCACTAGCCCTTTCAGGTAGCTATGCCTA1260.14691821552669015No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC1250.14575219794314498No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT1240.14458618035959983No Hit
GAAGGCTCTCTTACGAGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG1220.1422541451925095No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT1210.14108812760896433No Hit
ACAAGTGCCATTGCTGGAGGGGTACTTTCATACCCTGTCACTTGCCTTTC1190.13875609244187403No Hit
GCAGGTTCCTTCAAAGAAGGATCACTAGCCCTTTCAGGTAGTTATGCCTA1180.13759007485832886No Hit
GCAGGCTCCTTTCGGCCACGGCGTTTCCGCCGCTTCACCTGCATGTCAAG1170.1364240572747837No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATCCCTACCACCTGTATG1170.1364240572747837No Hit
TACAGTTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAACTGCAATATCA1140.13292600452414824No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT1120.13059396935705792No Hit
CACTTTGTCCCCGAAGGGAAAGTCCTATCTCTAGGATTGTCAAAGGATGT1110.12942795177351274No Hit
GTGGACTAACCCTGCTTTCACAGGGCTGACCCCGCCCGAAGGCGGAGCTC1110.12942795177351274No Hit
GTGACAGCTCCTTGCGGAGAGGGTGGCTTTCACCACCTGTCTGCCCCTCT1110.12942795177351274No Hit
GAAGGCTCCCTTACGGGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG1080.12592989902287727No Hit
ATACCTGTCCGAAGAAGGGTGTATCTCTACACCTGTCAGGTACAGTTCAA1070.12476388143933212No Hit
CTTCCTGCCCCGAAAGGCTTCCCCGGTTAAGGGTAATTCAGGAGATGTCA1070.12476388143933212No Hit
CGGAAAATCAGGTAAAGTCATCAGCTTGACCTTCATATATCCGTCGCTCC1060.12359786385578696No Hit
GCAAGCTCCCCGAAGGGTCGATCCACTTTCGCTTCTCTACCACTTGCATG1040.12126582868869663No Hit
GCAGGCTCCTCTCGGCCACGGCGTTTCCACCGCTTCACCTGCATGTCAAG1030.12009981110515146No Hit
CAGCTAGTCAAGCAGAGTCTTCAGCCCGGCTATCATTCAGCTGTCTTATC1020.1189337935216063No Hit
GAAACTGTTTTCATATTGCTATGAAAAAGGATCTAGTTTCTTAGATCTTT1020.1189337935216063No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATATTGCTGTCGTCCC1010.11776777593806113No Hit
ATAGGCTCCCCGAAGGGTCGGTCGCATCTCTGCTCCCTACCACCTATATG990.11543574077097082No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA990.11543574077097082No Hit
CTTCCTGCCCCGAAGGGCTTCCCCGATTAAGGGTAATTCAGGAGATGTCA980.11426972318742566No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCACTCTGCTGTCGCCCC980.11426972318742566No Hit
CAGCTCGTCTAGCAAGACCTTCAATCTGGCCTTCACTCTGCTGTCGATCC960.11193768802033534No Hit
GCAAAGCCCTTTCGGACACGACGTTTCCGCCGCTTTTCCTTGCATGTCAA950.11077167043679018No Hit
CACCTCTGCCCGAAGGAGATCCCGGTTAAGGGACTGTCAGAGGGATGTCA940.10960565285324503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCA100.0017806899230.000021
GAGGCGT100.0017806899230.000024
ATCCCAG100.0017806899230.000021
ATGGGCC100.0017806899230.000022
TCCTTGT100.0017806899230.000022
CATTGCC400.0230.000024
CAGTCCG202.1285814E-7230.000021
AGCTTGT800.0230.000022
TGTCCTT100.0017806899230.000026
ATAAGCT100.0017806899230.000021
GCCTATT202.1285814E-7230.000027
GCAGGTC100.0017806899230.000021
GGTCCCA100.0017806899230.000026
CACAAGG202.1285814E-7230.000029
CATCCGG100.0017806899230.000026
ATAGGCT400.0230.000021
ACCGCTC202.1285814E-7230.000024
CCAGATA100.0017806899230.000024
TTCGAAA202.1285814E-7230.000029
CCTTGTC202.1285814E-7230.000023
Produced by FastQC (version 0.11.9)