FastQCFastQC Report
Wed 30 Nov 2022
4061-JW37trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW37trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196
Sequences flagged as poor quality0
Sequence length231
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGTGGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCAC3115.816326530612246No Hit
AAGAGTGGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCAC2412.244897959183673No Hit
AAGAGTGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACG115.612244897959184No Hit
AAGAGTGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACG94.591836734693878No Hit
AAGAGTGGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA73.571428571428571No Hit
TGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTGT73.571428571428571No Hit
AAGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC63.061224489795918No Hit
AGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACC42.0408163265306123No Hit
AAGAGTGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAG42.0408163265306123No Hit
AGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT42.0408163265306123No Hit
AAGAGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAG42.0408163265306123No Hit
AAGAGTAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA31.530612244897959No Hit
GGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTT31.530612244897959No Hit
AAGAGTGGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA31.530612244897959No Hit
AGAGTGGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACG21.0204081632653061No Hit
AAGAGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACC21.0204081632653061No Hit
AGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTGT21.0204081632653061No Hit
GGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTT21.0204081632653061No Hit
AAGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC21.0204081632653061No Hit
GAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG21.0204081632653061No Hit
GTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTGTCC21.0204081632653061No Hit
AAGAGTGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAG21.0204081632653061No Hit
AAGAGTGGAGGAGGTGCATGGCCGTCTCTTATACACATCTCCGAGCCCAC21.0204081632653061No Hit
AAGAGTGGGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACG21.0204081632653061No Hit
AAGAGTGGTGCATGGCTGTCTCTTATACACCTCTCCGAGCCCACGAGACC10.5102040816326531No Hit
AAGAGTGGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGCGCCCAC10.5102040816326531No Hit
AAGAGTGGAGGAGGCGCATGGCTGTCACTTATACATATCTCCGAGCCCAC10.5102040816326531No Hit
CCGTATCATTGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCC10.5102040816326531No Hit
AAGCGTAGGAGGTGTATGGCTGTCTCTTATACACATCTACGAGCCCACGA10.5102040816326531No Hit
AAGAGTTGGGAGGTTCATTGCTGTCTCTTATACACATCTCCGATTCCACG10.5102040816326531No Hit
GAGTGGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA10.5102040816326531No Hit
AAGAGAGTACGAGGTGCATGGCTGCCTCTTATACACATCTCCGAGCCCAC10.5102040816326531No Hit
GGTAGTGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACG10.5102040816326531No Hit
AAGAGTGGAGGAGGTGCATGGCTGTCTCTTGTACACATCCCGGGGGCCAC10.5102040816326531No Hit
AAGAGTGGAGGTGGTGCATGGCTGTCTCTTCTACACATCTCCGAGCCCAC10.5102040816326531No Hit
AAAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACC10.5102040816326531No Hit
AAGGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA10.5102040816326531No Hit
AGGGAAAGAGTGGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAG10.5102040816326531No Hit
AAGAGGGGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10.5102040816326531No Hit
AAGAGGAGGAGCATGGCTGTCTCTTATACACATCTTCGAGCCCACGAGAC10.5102040816326531No Hit
ACGCGGGGAGGGGGGGATTGGCTGTCTCGTATATATAGCTCCGAGCCGCC10.5102040816326531No Hit
AAGAGGAGGTGCATGGCTGCTTCTTATACGCCTCTTCGATCCCACGAGCC10.5102040816326531No Hit
AAGGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACC10.5102040816326531No Hit
ATGCCGGAAGGGGGGGCATCGATGTCTCTTGGACACATCTCCGAGCCCAC10.5102040816326531No Hit
GAGGTGGTGCATGGCTGTCTCTTATACACATCTCCTAACCCACGAGACCG10.5102040816326531No Hit
AAGAGGGAGGCGGTGCATAGCTGTCGCTTATACACATCACCGAGCCCACG10.5102040816326531No Hit
AAGAGTGAGGAGGTGCAGGGCTGGCTTTTATACACATCACCGAGCCCACG10.5102040816326531No Hit
AAGAGGGGAGGAGGGGCATGGCGGTCTCTTGGACACAACTCCGGGCCCAC10.5102040816326531No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA10.5102040816326531No Hit
ATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTTGTCCATCT10.5102040816326531No Hit
AAGAGTTGAGGAGTTGCATGGCTGTCTCTTATACACATCTCCGAGCCCAC10.5102040816326531No Hit
GAGCCGCATGGCTGGCTCTTATACACATCTCCGTGCCCACGAGACCGGTG10.5102040816326531No Hit
AGGAGTGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAG10.5102040816326531No Hit
AGAGTGGGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA10.5102040816326531No Hit
AAGAGTGGAGGAGGTGCATGGCTGTCTCTGATACAACTCTCCGAGCCCAC10.5102040816326531No Hit
AAGAGGGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACG10.5102040816326531No Hit
AGAGTGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA10.5102040816326531No Hit
ACGCGGTGGAGCATGGCTGTCTCTTATACACATCTCCGAGCACACGAGAC10.5102040816326531No Hit
GAGGTGCATGGCTGACTCTTATACACATCTCCGAGCCCACGAGAACGGTG10.5102040816326531No Hit
AAGACTGGCGGTATTTCATGTCTGTCTTTTATCCACATCTCCCATCCCAC10.5102040816326531No Hit
TCGTCGGCAGCGTCAGAGGTGGTGCATGGCTGTCTCTTATACACATCTCC10.5102040816326531No Hit
ACGCCGTAAACGATGAGTACTAGGTGTGGGAGGTATTGATCAGTTTCGCG10.5102040816326531No Hit
AGAGTGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA10.5102040816326531No Hit
AAGAGTAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA10.5102040816326531No Hit
AGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACC10.5102040816326531No Hit
ACGAGGTGGAGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10.5102040816326531No Hit
GCCATCGCCCTGATAGAGGAGGTGCATGGCTGTCTCTTATACACATCTCC10.5102040816326531No Hit
AAGAGTGGTGCATGGCTGTCTCTTATACACAGCTCCGAGCCCACGAGACC10.5102040816326531No Hit
AAAGTGGAGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA10.5102040816326531No Hit
AAGGTGGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACG10.5102040816326531No Hit
CAGAGTAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA10.5102040816326531No Hit
AGAGTGGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACG10.5102040816326531No Hit
AAGAGTGGAGGTGCATGGCTGTCTCTTATACACACCTCCGGGCCCACGAG10.5102040816326531No Hit
AGAGTTGAGGGGGTGCATTGCTGTCTCTTATACACATCTCCGAGCCCACG10.5102040816326531No Hit
TAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG10.5102040816326531No Hit
AAGCGGTGGTGCATGGCTGTCTCTTATACACATCTCTGAGCACACGAGAC10.5102040816326531No Hit
GGCTGACTGACTTATAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA10.5102040816326531RNA PCR Primer, Index 46 (96% over 28bp)
AAGAGTGGGAGGTGCATGGCTGTATCTTATACACATCTCCGAGCCCACGA10.5102040816326531No Hit
AAGAGGGGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCTCACGA10.5102040816326531No Hit
GAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG10.5102040816326531No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA700.005556033516.0714385-89
Produced by FastQC (version 0.11.9)