FastQCFastQC Report
Wed 30 Nov 2022
4061-JW38trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW38trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137
Sequences flagged as poor quality0
Sequence length231
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGTGGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCAC1611.678832116788321No Hit
AAGAGTGGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCAC1611.678832116788321No Hit
AAGAGTGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACG139.48905109489051No Hit
AAGAGTGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACG96.569343065693431No Hit
AAGAGTGGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA32.18978102189781No Hit
AAGAGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAG32.18978102189781No Hit
AAGAGTGGAGGAGGTGCATGGCCGTCTCTTATACACATCTCCGAGCCCAC32.18978102189781No Hit
AGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGG21.4598540145985401No Hit
AGAGTGGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACG10.7299270072992701No Hit
AAGAGTGGAGGAGGGGCATGGCTGTCTCTTATACACCTCTCCGAGCCCTC10.7299270072992701No Hit
AGAGTGGGTGCATGGCTGTCTCTTATACACCTCTCCTAGCCCACGAGACT10.7299270072992701No Hit
AGAGTGTGGCGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA10.7299270072992701No Hit
ACGCCCTAAACGATGGGGTCTAGATTTAGGGGGTATCGACCCCTCCCGTG10.7299270072992701No Hit
AAGAGGGGAGGAGGGGCAAGGCTGTGTCTTATACACATATCCGAGCACAC10.7299270072992701No Hit
AAGAGTGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAG10.7299270072992701No Hit
AAGAGGGGAGGTGGTGCATGGCTGTCTCTTATACACATCGCCGAGCCCAC10.7299270072992701No Hit
AGAGGGGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAG10.7299270072992701No Hit
AAGAGTGGAGGTGATGCATGTCTGTCTCTTCTACACATCGCCGAGCCCAC10.7299270072992701No Hit
AAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTG10.7299270072992701No Hit
TGGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10.7299270072992701No Hit
TGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGAAC10.7299270072992701No Hit
AAGAGTGGAGGTGGTGCATGGCAGTCTCTTATACACATCTCCGAGCCCAC10.7299270072992701No Hit
AAGACTGGAGTTGGTGCTTCTGTGTGTTTGACATTTTTCTCGGTGTCACA10.7299270072992701No Hit
TGGGCTCGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA10.7299270072992701No Hit
AAGAGTGGAGGAGGTGCATGGCTGTCTCTTCTACACATCTCCTCGCCAAC10.7299270072992701No Hit
AAGAGTGAGGAGGTGCATTGCTGTCTCTTCTACACATCTCCGATCCTACG10.7299270072992701No Hit
AGAGGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAG10.7299270072992701No Hit
TAGAGTGGAGGAGGTGCATGGCTGTTTCTTATACACATCTCCGAGCCCGA10.7299270072992701No Hit
AAGAGTGGAGGAGGGGCATGGCTGTCTCTTATACACATCTCCGAGCCCAC10.7299270072992701No Hit
AAGAGTGAGGAGGTGCATGGCTGTCTCTTATACACATCACCGAGCCCACG10.7299270072992701No Hit
AAGAGTGGAGGAGGTGCATGGGTGACTCTTATACACATATCCGAGGCCAA10.7299270072992701No Hit
ATGAGTGGTGGTGGTGCTTGGCTGCCTCTTATACACATCTCCGAGCTCAC10.7299270072992701No Hit
AGGTGGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA10.7299270072992701No Hit
AAGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGAAC10.7299270072992701No Hit
ACGCCGTAAACAATGAATACTAGATTTTGAGCATATAGACCAGTGTCGCA10.7299270072992701No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCGGTGTCGCA10.7299270072992701No Hit
ACGCCTGAAACGGTGACTTCTAGATGTTTGACATATTGCTCAGTGTCGCA10.7299270072992701No Hit
CCGCCGGAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA10.7299270072992701No Hit
AGAGTGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA10.7299270072992701No Hit
TAGCGTGGAGGAGGTGCCTGGCTGTCTAATATACATCTACCCCTTTTGAC10.7299270072992701No Hit
GGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGA10.7299270072992701No Hit
AGGAGTGGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGGGCCCAC10.7299270072992701No Hit
ACGCGGTAAGAAGGGACTTCTCGATGTCGTACATATTGATCAGGGCCGCC10.7299270072992701No Hit
AAGAGTGGAGGAGGTGCATGGCTGTCTCTTGTACACATCTCCGAGCCCAC10.7299270072992701No Hit
GGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGA10.7299270072992701No Hit
GAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTG10.7299270072992701No Hit
TCGTCGGCAGCGTCAGAGGTGGTGCATGGCTGTCTCTTATACACATCTCC10.7299270072992701No Hit
AAGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGG10.7299270072992701No Hit
AGAGTGGAGGAGGTGCATGGCTGTCTCTTATGCACATCTCCGAGCCCACG10.7299270072992701No Hit
AGGAGGTGCATGGCTGTCTCTAATAGACATCTCCGAGCCCACGAGGCTGG10.7299270072992701No Hit
GAGAGTGGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCAC10.7299270072992701No Hit
TGGGCTCGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA10.7299270072992701No Hit
AGAGTGGGAGGTGCATGGCTGTCTCTTATACACATCTCCGGGCCCACGAG10.7299270072992701No Hit
AGGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTG10.7299270072992701No Hit
AAGAGGTGAGGTGGGGCATGGCTGTCTCTTATAGACATCTCCGTGCACAC10.7299270072992701No Hit
AAGAGTGGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA10.7299270072992701No Hit
CAGAGTGGAGGAGGTGCATGGCTGTCTCTTGTACACATCCCCGAGCCACG10.7299270072992701No Hit
AGGAGGAGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGG10.7299270072992701No Hit
ACGAGGTGAAGCATGACTGTCTCTTATACACATTACCGAGCCCACGAGCC10.7299270072992701No Hit
TCTTCCGATCTGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGC10.7299270072992701No Hit
AAGAGTGGAGGAGTTGCATGGCTGTCTCTTATACACATCTCCGAGCCCAC10.7299270072992701No Hit
GAGGTGGTGCATGGCTGTCTCTCATACACATCTCCGAGCCCACGAGACTG10.7299270072992701No Hit
AGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGG10.7299270072992701No Hit
AAGAGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACT10.7299270072992701No Hit
GTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGAA10.7299270072992701No Hit
AGAGTGGAGGAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACG10.7299270072992701No Hit
AAGACGTAAAGGGCGAGTCCTTCTCGTTTACCCTTTTCCTCGGTCACGCG10.7299270072992701No Hit
AAGAGTGGAGATGGGGGATGGCTGGCTCTTATAAACATCTGCGGGGCCAC10.7299270072992701No Hit
AAGAGTGAAGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAG10.7299270072992701No Hit
AAGAGAGGAGGCGGTGCATGGCTGCCTCTTATACACATCTCCGAGGGCAC10.7299270072992701No Hit
AGAATGAGGTGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGA10.7299270072992701No Hit
ACGAGCTGAGGTAGTGCAAGGCTGTCTCTTATACAGATCTCCCATTACAC10.7299270072992701No Hit
TGAAGATGATGTGCATGGCTGTCTCTTATACACCTCTCCGATCCCTCGCG10.7299270072992701No Hit
AAGAGTGGGAGGAGAATGGCTGTCTCTTATACACATCTCCGAGCCCACGA10.7299270072992701No Hit
CCGCCGTAGCCAGTGTTTTCTGGATGTAGTGGGTGTTAAGCGCTTGTAGG10.7299270072992701No Hit
AGGAGTGGAGGAGGTGCGTGGCTGTCTCTTATACCAATCTCCGAGGCCAC10.7299270072992701No Hit
AAGAGTGGGGGAGGGGTATGGGGGATTCTTGGACGTATAGCGGGTGCAGG10.7299270072992701No Hit
AGGTGCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGAAC10.7299270072992701No Hit
AAAGTGGCGGTGCATGGCTGTCTCTTATACACAACTCCGAGCCCACGAGA10.7299270072992701No Hit
AAGCGGGAGGCGGTGCATAGCTGACTCTTATACATATCTCCGGGCCCACG10.7299270072992701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTGG100.0225.07
GAGTGAG100.0225.03
AAGAGTG100.0225.01
GTGAGGT100.0225.05
AGGTGGT100.0225.08
AGAGTGA100.0225.02
AGTGAGG100.0225.04
TGAGGTG100.0225.06
GGTGGTG100.0225.09
AAAAAAA653.395362E-724.2307790-94
Produced by FastQC (version 0.11.9)