FastQCFastQC Report
Wed 30 Nov 2022
4061-JW38trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW38trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137
Sequences flagged as poor quality0
Sequence length236
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTTTCCCTACCCGGGTATCTAATCTGTCTCTTATACACATCTGACGC1611.678832116788321No Hit
ACTCTTTCCCTACACGGGTATCTAATCTGTCTCTTATACACATCTGACGC1410.218978102189782No Hit
ACTCTTTCCCTACGCGGGTATCTAATCTGTCTCTTATACACATCTGACGC85.839416058394161No Hit
CTCTTTCCCTACACGGGTATCTAATCTGTCTCTTATACACATCTGACGCT64.37956204379562No Hit
CTCTTTCCCTACGCGGGTATCTAATCTGTCTCTTATACACATCTGACGCT53.64963503649635No Hit
ACTCTTTCCCTACAGGGGTATCTAATCTGTCTCTTATACACATCTGACGC42.9197080291970803No Hit
ACTCTTTCACTACCCGGGTATCTAATCTGTCTCTTATACACATCTGACGC42.9197080291970803No Hit
CTCTTTCCCTACCCGGGTATCTAATCTGTCTCTTATACACATCTGACGCT32.18978102189781No Hit
ACTCTTTCCCTACCGGGGTATCTAATCTGTCTCTTATACACATCTGACGC32.18978102189781No Hit
CTTTCCCTACCCGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGC21.4598540145985401No Hit
CTTTCCCTACAGGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGC21.4598540145985401No Hit
ACTCTTTCACTACACGGGTATCTAATCTGTCTCTTATACACATCTGACGC21.4598540145985401No Hit
CTCTTTCACTACCCGGGTATCTAATCTGTCTCTTATACACATCTGACGCT21.4598540145985401No Hit
CCCTACCGGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCGAC21.4598540145985401No Hit
CTCTTTCACTACACGGGTATCTAATCTGTCTCTTATACACATCTGACGCT21.4598540145985401No Hit
CTCTTTCCCTACCGGGGTATCTAATCTGTCTCTTATACACATCTGACGCT21.4598540145985401No Hit
TCTTTCACTACGCGGGTATCTAATCTGTCTCTTATACACATCTGACGCTG21.4598540145985401No Hit
ACTCTTTCCTACCCGGGTATCTAATCTGTCTCTTATACACATCTGACGCT21.4598540145985401No Hit
ACTACCCGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCGACG21.4598540145985401No Hit
ACTCTCTCCCTACACGGGTATCTAATCTGTCTCTTATACACATCTGACGC21.4598540145985401No Hit
CCTACACGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCGACG10.7299270072992701No Hit
GACGCTGCCGACGAGGACTACACGGGTATCTAATCTGTCTCTTATACACA10.7299270072992701No Hit
CTCTTCCCTACCCGGGTATCTAATCTGTCTCTTATACACATCTGACGCTG10.7299270072992701No Hit
TCAGGACTACGGTTGTATCTAATCTGTCTCTTATACACATCTGACGCTGC10.7299270072992701No Hit
TCTCTCTCCCTACCCGGGTATCTAATCTGTCTCTTATACACATCTGACGC10.7299270072992701No Hit
ACTCTTTCCCTACAGGGGTATCTAATCTGTATCTTATACACATCTGACGC10.7299270072992701No Hit
TAATCCTGCTTGCTCCCCCCGCTTTTGCGTCTCAGTGTCCTTCACGGTCC10.7299270072992701No Hit
TCTTTCCCTACAGGGGTATCTAATCTGTCTCTTATACACATCTGACGCTG10.7299270072992701No Hit
ACTCTTTCCCTACACGGGTATCTACTCTGTCTCTTATACACATCTGACGC10.7299270072992701No Hit
CCTACGGGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCGACG10.7299270072992701No Hit
CCCTACAGGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCGAC10.7299270072992701No Hit
TAATCCTGTCCGCAGGGCAAGACTTCGTGACGGACTCTGACTTCCAGACT10.7299270072992701No Hit
CCCAGGACTACGGGGGTATCTAATCTGTCTCTTATACACATCTGACGCTG10.7299270072992701No Hit
TAATCCTGTCCGAACGCCACGATCTTGCTACTAAGCGTGATGACAGATCC10.7299270072992701No Hit
ACTCTTTCACTACGCGGGTATCTAATCTGTCTCTTATACACATCTGACGC10.7299270072992701No Hit
ACTACACGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCGACG10.7299270072992701No Hit
CTCTTTCCCTACGGGGGTATCTAATCTGTCTCTTATACACATCTGACGCT10.7299270072992701No Hit
CTCTTCCCTACACGGGTATCTAATCTGTCTCTTATACACATCTGACGCTG10.7299270072992701No Hit
CCCAGGACTACCGGGGTATCTAATCTGTCTCTTATACACATCTGACGCTG10.7299270072992701No Hit
TCTTTCCCTACCCGGGTATCTAATCTGTCTCTTATACACATCTGACGCTG10.7299270072992701No Hit
CTCTTTCCCTACACGGGTATCTAATCTGTCTCTTATCCACATCTGACGCT10.7299270072992701No Hit
ACACGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGA10.7299270072992701No Hit
TCTTTCCCTACACGGGTATCTAATCTGTCTCTTATACACATCTGACGCTG10.7299270072992701No Hit
CTCTTCCCTACCGGGGTATCTAATCTGTCTCTTATACACATCTGACGCTG10.7299270072992701No Hit
CTCTTTCCCTACACGGGCATCTAATCTGTCTCTTATACACATCTGACTCT10.7299270072992701No Hit
TACACCTGCCCGATGGCCCTCCTCTTGCACTTCATCGACATTCACTAGCC10.7299270072992701No Hit
TTTCCCTACCGGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCC10.7299270072992701No Hit
CTCTTTCCCTAGACGGGTATCTAATCTGTCTCTTATACACATCTGACGCT10.7299270072992701No Hit
TCTTCCCTACACGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGC10.7299270072992701No Hit
ACGCGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGA10.7299270072992701No Hit
GATCGGAAGAGGACTACAGGGGTATCTAATCTGTCTCTTATACACATCTG10.7299270072992701No Hit
TACCCGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG10.7299270072992701No Hit
CTCTTTCCCTACAGGGGTATCTAATCTGTCTCTTATACACATCTGACGCT10.7299270072992701No Hit
TCTTCCCTACGCGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGC10.7299270072992701No Hit
CTACCGGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCGACGA10.7299270072992701No Hit
CTACCCGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCGACGA10.7299270072992701No Hit
TTCCCTACACGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCG10.7299270072992701No Hit
TCTTCCCTACGGGGGTATCTAATCTGGCTCTTATACACATCTGACGCCTC10.7299270072992701No Hit
TCCCTACCCGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCGA10.7299270072992701No Hit
TCCCTACACGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCGA10.7299270072992701No Hit
ACCTTCCCTACGCGGGTATCTAATCTGTCTCTTATACACATCTGACGCTG10.7299270072992701No Hit
GACTACCCGGGTATCCAATCTGTCTCTTATACACATCTGACGCTGCCGAC10.7299270072992701No Hit
ACTCTCTCACTACACGGGTATCTAATCTGTCTCTTATACACATCTGACGC10.7299270072992701No Hit
ACCCGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGA10.7299270072992701No Hit
ATTCCTACACGGGTATCTAATCTATCTCTTATACACATCTGACGCTGCCG10.7299270072992701No Hit
TACCCCTGGGGGTTGCTCACGCTCTCTCGTCTCATAGTGAGTCTAGGGCC10.7299270072992701No Hit
CTTTCCCTACCGGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGC10.7299270072992701No Hit
ACTCTTCCCTACCGGGGTATCTAATCTGTCTCTTATACACATCTGACGCT10.7299270072992701No Hit
ACTCTCTCCCTACGGGGGTATCTGATCTGTCTCCTATACACATCTGACGC10.7299270072992701No Hit
TCACTTTGCCTACCGGGGAATCTAATCTGTCTCTTAGACACTTCCGACCC10.7299270072992701No Hit
CTTTCCCTACACGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGC10.7299270072992701No Hit
ACTACAGGGGTATCTAATCTGTCTCTTATACACATCTGACGCTGCCGACG10.7299270072992701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCCC100.0230.000023
CCTACCC100.0230.000028
TTTCCCT100.0230.000024
CTCTTTC100.0230.000021
TTCCCTA100.0230.000025
TCTTTCC100.0230.000022
CTACCCG100.0230.000029
CCCTACC100.0230.000027
TCCCTAC100.0230.000026
AAAAAAA756.6302164E-924.53333595-99
Produced by FastQC (version 0.11.9)