FastQCFastQC Report
Mon 28 Nov 2022
4061-JW01trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW01trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63989
Sequences flagged as poor quality0
Sequence length233
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGGAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGCA940014.690024848020752No Hit
TACGGAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGTA61689.639156730062979No Hit
GACGGAGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA48757.618496929159699No Hit
AACGAGGGGGACGAGCGTTATTCATCATGACTGGGCGTAAAAGGTTCGTA24533.8334713778930753No Hit
TACGAAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA14842.3191486036662554No Hit
TACGGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGAGTCCGTA11241.7565519073590774No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTA8891.3893012861585585No Hit
TACGAAGGTGGCAAGCGTTGTTCGGATTTACTGGGCGTACAGGGAGCGTA8521.3314788479269875No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCACTGGGTTTAAAGGGTGCGTA8311.2986607073090688No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA7901.2345871946740847No Hit
GACGGAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGTA6991.092375251996437No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA6751.0548688055759583No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA5850.9142196314991639No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA5660.8845270280829518No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA5450.851708887465033No Hit
TACGGAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGCA5280.8251418212505274No Hit
TACGGAGGATGCAAGCGTTATCCGGAATCATTGGGCGTAAAGCGTCCGCA5230.817327978246261No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA4700.7345012424010376No Hit
TACAGAGAGTGCAAACGTTGTTCGGAATTACTGGGCTTAAAGCGCACGTA4660.7282501679976245No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA4400.6876181843754395No Hit
TACGAAGGTCCCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGAGCGTA4010.626670208942162No Hit
CACGTAGGCACCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA3650.5704105393114441No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA3030.4735188860585413No Hit
TACAGAGGGTGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA3020.47195611745768806No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCGTGTA2890.4516401256465955No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA2890.4516401256465955No Hit
TACGTAGGTGACAAATGTTATCCGGAATCACTGGGCGTAAAGGGTCTGTA2770.43288690243635625No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA2420.3781900014064918No Hit
TACAGAGACTGCAAGCGTTATTCGGATTCACTGGGCGTAAAGGGTGCGCA2330.36412508399881227No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA2290.3578740095953992No Hit
GACGAACCGTCCAAACGTTACTCGGAATTACTGGGCTTAAAGGGTGCGTA2150.33599524918345336No Hit
GACGGAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTACGTA2110.3297441747800403No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA2090.3266186375783338No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGAGTGTA2000.3125537201706543No Hit
GACGGGGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA1920.30005157136382815No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA1820.28442388535529545No Hit
GACAGAGGGTGCAAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA1670.26098235634249634No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGCGCACGCA1560.24379190173311038No Hit
TACGAAGGTCCCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGAGCGTA1550.2422291331322571No Hit
TACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA1540.24066636453140383No Hit
GACAGAGGGTGCAAACGTTGCTCGGAATCACTGGGCGTAAAGGGCGTGTA1540.24066636453140383No Hit
TACAGAGGCCACAAGCGTTAGTCGGAATCACTGGGCTTAAAGGGTGCGTA1470.22972698432543096No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATCACTGGGCATAAAGCGCACGTA1450.22660144712372438No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATCACTGGGCTTAAAGCGTTCGTA1440.2250386785228711No Hit
TACGAATGGGGCAAGCGTTGTTCGGAATCACTGGGCTTAAAGCGTGCGTA1370.2140992983168982No Hit
TACGGAGGGGGCGAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA1330.20784822391348512No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTGCGTA1320.20628545531263184No Hit
TACGGAGGAGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGTA1240.1937833065058057No Hit
AACGTAGGACGCAAGCGTTATCCGGAATTACTGGGCGTAAAGCGCGTGTA1230.19222053790495242No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGTA1190.1859694635015393No Hit
TACGGAGGGGGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA1190.1859694635015393No Hit
GACGAACCGTGCGAACGTTGTTCGGAATCACTGGGCTTAAAGGGCGCGTA1140.17815562049727296No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA1070.16721624029130008No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTACGTA1040.16252793448874026No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGTGTA1030.16096516588788698No Hit
GACGGAGGATGCAAGCGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA1020.1594023972870337No Hit
TACAGAGGGTGCAAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA1010.15783962868618046No Hit
TTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTA1000.15627686008532715No Hit
TACGAAGGTGGCAAGCGTTGTTCGGATTCACTGGGCGTACAGGGTGTGTA960.15002578568191408No Hit
TACGGAGGGTACGAACGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA950.1484630170810608No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA950.1484630170810608No Hit
AACGTAGGAGGCGAGCGTTATCCGGAGTTACTGGGCGTAAAGAGCGTGTA950.1484630170810608No Hit
TACGGAGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA930.14533747987935428No Hit
TACGAAGGTGGCAAGCGTTGTTCGGATTCACTGGGCGTACAGGGAGCGTA900.14064917407679445No Hit
TACGTAGGGTCCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA890.13908640547594117No Hit
TACAGAGGGTGCGAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA850.1328353310725281No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA840.13127256247167482No Hit
GACGAACCGAGCGAACGTTGTTCGGAATCACTGGGCTTAAAGGGCGCGTA810.126584256669115No Hit
GACGAGCCTCACAAACGTTATTCGGAATTACTGGGCTTAAAGGGTGCGTA780.12189595086655519No Hit
TACAGAGGTCTCAAGCGTTGTTCGGATTCATTGGGCGTAAAGGGTGCGTA760.11877041366484865No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGTA760.11877041366484865No Hit
TACGGGGGGGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGGGCGCGTA760.11877041366484865No Hit
TACGAAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA740.11564487646314209No Hit
TACGGAGGATGCAAGCGTTATCCGGAATTATTGGGCGGAAAGCGTCCGTA740.11564487646314209No Hit
TACAGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA730.11408210786228884No Hit
TACGTAGGGGCCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGTGGGTA720.11251933926143555No Hit
TACGTAGGGTGCAGGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA710.1109565706605823No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA700.10939380205972901No Hit
TACGGAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGGA680.10626826485802249No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATGACTGGGCGTAAAGGGCGCGTA670.1047054962571692No Hit
TACGTAGGCAGCAAACGTTGTTCGGAATTACTGGGCGTAAAGAGTGTGTA640.10001719045460938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAAA100.0018509697227.000029
CCAGCGC100.0018509697227.000023
AACGAGG3050.0227.000021
CACGTAC152.0510428E-5227.000021
GGTCCCG500.0227.000027
TGACAAA202.2680797E-7227.000029
ACCGTAC152.0510428E-5227.000026
CCGTCCA400.0227.000027
AGAATGC152.0510428E-5227.000026
AGGGACC152.0510428E-5227.000026
GTCCCGA500.0227.000028
GCAGCAA252.5211193E-9227.000028
ACGAGCC100.0018509697227.000022
GAACCGA152.0510428E-5227.000024
GACGCAA252.5211193E-9227.000028
CGAATGG152.0510428E-5227.000023
CGTACGA152.0510428E-5227.000028
GTCTCAC100.0018509697227.000026
CCACAAG252.5211193E-9227.000029
TACGAAT152.0510428E-5227.000021
Produced by FastQC (version 0.11.9)