FastQCFastQC Report
Mon 28 Nov 2022
4061-JW01trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW01trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63989
Sequences flagged as poor quality0
Sequence length232
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTGTGGTCCAGC801812.530278641641532No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA57909.048430198940443No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTGATGGCCCAGCA40086.263576552219913No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTAATGGCCCAGCA38506.016659113285096No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGATAAGGTCCAGCA33955.305599399896857No Hit
CCTGTTTGCTACCCATACTTTCGTTCCTCAACGTCAGTTTTTACTAGAGA27894.358561627779775No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATCACGGGCCAGAT16222.5348106705840068No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTTATAGCCCAGCA12441.9440841394614699No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG9751.5236993858319399No Hit
CATCTTCGATCCCCTAACTTTCGTTCTTGATTAATGAAAACATCCTTGGT9411.4705652534029285No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTTCTGGTCCAGTA8271.2924096329056556No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATAAAGGCCCAGTT6951.0861241775930237No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTTAGTAATAGCCCAGTA6531.0204878963571864No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTGCAGCCCAGCA5250.8204535154479676No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACTGGTCCAGGT5010.7829470690274891No Hit
CCCGTTTGCTCCCCTAGCTTTCGCACCTCAGCGTCAGTTGTGGACCAGAG4850.7579427714138368No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG4470.6985575645814124No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCGCTGTCCAGAA3680.575098845114004No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG3550.5547828533029114No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGTAAATGTCCAGTA3160.49383487786963387No Hit
CCTGTTTGCTACCCACGCTATCGTCCCTCAGCGTCAATCATCGTCTAGCC2850.4453890512431824No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCACTACAAGTCCAGCA2810.43913797683976935No Hit
CCCGTTTGCTACCTTAGCTTTCGTGCCTCAGCGTCAGAAGAGACCCAGTA2640.4125709106252637No Hit
CCCATTCGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTGAGATCCAGCA2600.40631983622185064No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGGT2440.3813155386081983No Hit
CCCTTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGCAATTGTCCAGGA2340.3656878525996656No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTAGCTGTCCAGAA2280.3563112409945459No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACGGCCCAGAG2210.345371860788573No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGATCCAGTG2190.3422463235868665No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTGTGGTCCAGC2140.33443248058260017No Hit
CCTGTTTGCTCCCCACGCTTTCGAGTCTCAGCGTCAGTGTTCGTCCAGTT2090.3266186375783338No Hit
CCTGTTTGATCCCCACGCTTTCGCGCTGCAGCGTCAGAAACTGGCCAGTG1940.3031771085655347No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGGACAGGCCCAGCC1900.2969260341621216No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAATACTTGTCCAGTG1900.2969260341621216No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTATCGTCCAGAA1850.28911219115785525No Hit
CCCGTTTGCTCCCCACGCTTTCGTTCCTCAGCGTCAGTACAAGCCCAGCG1820.28442388535529545No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCTGTGAGGGTCAGTTGTGGTCCAGC1720.26879619934676274No Hit
CCCGTTTGCTACCCTAGCTTTCGCACCTCAGCGTCAGATTTGGCCCAGTG1680.26254512494334964No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAAAAATTGCCCAGTT1650.2578568191407898No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATCTAGGTCCAGCA1550.2422291331322571No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTTTCGGCCCAGAG1490.23285252152713745No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG1480.23128975292628418No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTCAGCGTCAGGTTGGTCCCAGGG1370.2140992983168982No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAGTTACAGTCTAGTA1360.21253652971604498No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATCAGCGTCAGAACCGGTCCAGGT1360.21253652971604498No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA1340.2094109925143384No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTACCGTCCAGAA1340.2094109925143384No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCATCGTCCAGAA1290.20159714951007204No Hit
CGTCTTCGAGCCCCCAACTTTCGTTCTTGATCAATGAAAACATCCTTGGC1270.1984716123083655No Hit
CCCGTTCGCTCCCCACGCTTTCGCACCTGAGCGTCAGGCCAAGGCCAGGG1260.19690884370751222No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG1260.19690884370751222No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTT1230.19222053790495242No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTCTTAGTCCAGGG1230.19222053790495242No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCTTAGTA1180.18440669490068606No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA1170.1828439262998328No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG1160.1812811576989795No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATCGACCCAGGG1130.1765928518964197No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTTCTGGACCAGGA1130.1765928518964197No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTGATGGCCCAGCA1060.1656534716904468No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTTGGCCCAGTT1040.16252793448874026No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG1020.1594023972870337No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCTACGGCCCAGCA1010.15783962868618046No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGTGTCAGTGACCGTCCAGAT1000.15627686008532715No Hit
CCCTTTTGCTCCCCTGGCCTTCGAGCCTCAGCGTCAGATGTTGTCCAGTA990.1547140914844739No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG980.15315132288362063No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTAATGGCCCAGCA970.15158855428276735No Hit
CCCGTTCGCTCCCCGTGCTGTCGCGTCTCAGCGTCAGGTCAAGCCCAGGA960.15002578568191408No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGTATGTTCCAGAG950.1484630170810608No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTGCCGTCCAGGT950.1484630170810608No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGATAAGGTCCAGCA940.14690024848020752No Hit
CCCGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAATTGTCCAGGG940.14690024848020752No Hit
CCTGTTTGCTCCCCACGCCGTCGTGGCTCAGTGTCAGGTCTCGCCCAGAA920.143774711278501No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGTCTAGTA870.13596086827423465No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGAT870.13596086827423465No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATGTCGGTCCAGAT850.1328353310725281No Hit
CCTGTTTGCTACCCGCGCTTTCGCGTCTCAGCGTCAGTACCTGTCCAGGT830.12970979387082154No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGA810.126584256669115No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGAACCGGCCCAGGT740.11564487646314209No Hit
CCCGTTTGCTACCCTAGCTTTCGTGCATCAGCGTCAGAAGAGATCCAGAA740.11564487646314209No Hit
CCCGTTCGCTACCCACGCTTTCGTCCCTGAGCGTCAACAACGGCCCAGCC730.11408210786228884No Hit
CCTGTTTGCTCCCCACGCTTTCGACCATGAGCGTCAGTATCGGGCCAGAG720.11251933926143555No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGATCCGGTCCAGGT720.11251933926143555No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGGAGTTGTCCAGTA700.10939380205972901No Hit
CCTGTTTGATCCCCACGCTTTCGCGCTACAGCGTCAGAAACGAGCCAGTG700.10939380205972901No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTTGTGGTCCAGCA700.10939380205972901No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGTTGGACCAGGA700.10939380205972901No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCGGGCCAGTG690.10783103345887574No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGG690.10783103345887574No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTACTGTCCAGGA680.10626826485802249No Hit
CCTGTTTGCTACCCATACTTTCGTTCATCAACGTCAGTTTTTACTAGAGA680.10626826485802249No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTTAGCGTCAGTACCGGTCCAGAT680.10626826485802249No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTGAGCGTCAGTATTGGGCCAGGA680.10626826485802249No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGATGGGGTCCAGCG680.10626826485802249No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG660.10314272765631592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGC2600.0226.000023
CCTGTTC650.0226.000021
CTTCGAT1100.0226.000024
TCTTCGA1100.0226.000023
TTTGCTC20200.0226.000025
TTTGCTA12700.0226.000025
CTTTTCG100.0018755944226.02
TTTTGCT800.0226.04
GTTTGAT2650.0226.04
TTTTCGC100.0018755944226.03
TGTTCGC600.0226.03
TGTTCGA100.0018755944226.03
TGTTTGC22050.0226.03
TGTTTGA2650.0226.03
GGTTTGC100.0018755944226.03
CATTTGC7350.0226.03
TGCTCCC20300.0226.07
CCTTTTG800.0226.02
CCATTCG12800.0226.02
ATTTGCT7350.0226.04
Produced by FastQC (version 0.11.9)