FastQCFastQC Report
Mon 28 Nov 2022
4061-JW02trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW02trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123846
Sequences flagged as poor quality0
Sequence length232
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA5873447.42502785717746No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA112809.108085848553849No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA57644.654167272257482No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA20231.6334802900376273No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTGTGGTCCAGC11930.9632931221032573No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA10390.8389451415467597No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT9050.7307462493742228No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA7580.6120504497521115No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT7220.5829820906609823No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT7050.5692553655346155No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA6040.4877024691956139No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG5680.45863411010448457No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA4700.3795035770230771No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA4650.3754663049270869No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACTCGACCAGAA4060.32782649419440274No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT3190.2575779597241735No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA3110.2511183243705893No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG3090.2495034155321932No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA2930.23658414482502466No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGTA2920.2357766904058266No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA2770.22366487411785602No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG2610.21074560341068746No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA2600.20993814899148944No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAGTATTGGTCCAGGA2530.20428596805710317No Hit
CCCGTTTGCTACCCTAGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGAG2440.19701887828432085No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA2250.18167724431955817No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGTGTCAGTTACGGACCAGGA2080.16795051919319154No Hit
CCTGTTTGCTACCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA2080.16795051919319154No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGTGTCAGGTCAGGCCCAGGT1990.16068342942040922No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA1980.15987597500121117No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA1930.155838702905221No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTTGGCCAGGT1900.1534163396476269No Hit
CCGGTTCGTGCCCCCAGCTTTCGTCCCTCACCGTCGAACCCGTTCTGGTA1900.1534163396476269No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG1880.1518014308092308No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTTCGGTCCAGGA1860.15018652197083474No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG1860.15018652197083474No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA1800.14534179545564654No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGCGTCAGTACCGGGCCAGTG1770.14291943219805242No Hit
CCCGTTTGCTACCTTAGCTTTCGTGTCTGAGCGTCAGAAATGGGCCAGGA1730.13968961452126027No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATAGGGCCAGAA1710.1380747056828642No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT1700.13726725126366618No Hit
CCCGTTTGCTACCCTAGCTGTCGCGTCTGAGCGTCAGAAATGGTCCAGAA1630.1316150703292799No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG1570.1267703438140917No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA1540.12434798055649758No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGTATCGGTCCAGAA1510.12192561729890347No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTGCAGACCTAGCG1480.11950325404130938No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGAT1430.1154659819453192No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTTCTCGACCAGAA1430.1154659819453192No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTTACAGCTTAGTG1350.1090063465917349No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA1340.10819889217253686No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA1250.10093180239975454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGC6350.0225.99723
TTTTGCT100.0018777807225.997184
GTTTGTT100.0018777807225.997184
TTACTAC100.0018777807225.997186
TTCGATC2250.0225.997185
TGTTCGC1900.0225.997183
TGTTTGT100.0018777807225.997183
GGTTCGC1600.0225.997183
GGTTTGC400.0225.997183
GTTTACT100.0018777807225.997184
CCATTCG1200.0225.997182
TTGATAC152.0923057E-5225.997186
TTTACTA100.0018777807225.997185
TTTGCTG202.3274333E-7225.997185
TTTGCGC100.0018777807225.997185
CTTTTTG100.0018777807225.997182
CGATCCC2250.0225.997187
GTGCCCC302.910383E-11225.997188
TTTGATA152.0923057E-5225.997185
CGTTCAC252.6029738E-9225.997163
Produced by FastQC (version 0.11.9)