FastQCFastQC Report
Mon 28 Nov 2022
4061-JW03trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW03trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51505
Sequences flagged as poor quality0
Sequence length233
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA2276344.19570915445102No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA12142.357052713328803No Hit
GACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCTTAAAGGGCGCGTA11632.25803320066013No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGTGTA7651.4852926900300942No Hit
TACGGAGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA7481.4522861858072031No Hit
TACGGAGGGGGCAAGCGTTACTCGGAATTATTGGGCGTAAAGGGCGCGTA6081.1804679157363362No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA5241.0173769536938162No Hit
TACAGGGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA4890.9494223861760994No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA4610.895058732161926No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGGGTAAAGGGTGCGCA3590.6970197068245801No Hit
CACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA3490.6776041161052325No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGGGCGCA3090.5999417532278419No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGCA2680.5203378312785166No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGTGTA2630.5106300359188428No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGCACGTA2560.4970391224152995No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2220.4310261139695175No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA2100.40772740510630034No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGCGTA1810.3514221920201922No Hit
TACAGGGGAGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA1800.34948063294825743No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA1690.32812348315697504No Hit
GACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCGTGTA1640.31841568779730123No Hit
TACGTAGGGGGCAAGCGTTACTCGGATTCATTGGGCGTAAAGCGAATGTA1560.3028832152218231No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1550.3009416561498883No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA1530.29705853800601884No Hit
TACGTAGGATGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGAGTA1520.29511697893408406No Hit
CACGTAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA1450.2815260654305407No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA1280.24851956120764976No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA1260.2446364430637802No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA1170.22716241141636737No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1170.22716241141636737No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA1140.22133773420056307No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGTACGTA1040.2019221434812154No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGTGCGCA990.1922143481215416No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA980.19027278904960682No Hit
CACGTAGGAGGCGAGCGTTATCCGGATTCACTGGGCGTAAAGCGCGTGCA950.18444811183380255No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGTGTA940.18250655276186778No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA940.18250655276186778No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA930.180564993689933No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGGAAAGGGTGCGCA910.1766818755460635No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGTGTGCA880.1708571983302592No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA800.1553247257547811No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA790.15338316668284635No Hit
TACGTAGGATCCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGCGTGTA790.15338316668284635No Hit
TACGTAGGGAGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGCGTA740.1436753713231725No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGAGCGTGTA720.13979225317930297No Hit
CACGTAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA720.13979225317930297No Hit
TACAGAGGGGGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA720.13979225317930297No Hit
GACGGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA710.13785069410736822No Hit
TACGTATGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA710.13785069410736822No Hit
TACAGGGGTGGCAAGCGTTGTCCGGCTTTACTGGGTGTAAAGGGTGCGCA680.13202601689156393No Hit
TACGGGGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA670.13008445781962916No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA660.1281428987476944No Hit
TACAGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA650.12620133967575964No Hit
TACAGAGGTGTCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGCGTGCA650.12620133967575964No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA640.12425978060382488No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA630.1223182215318901No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGTA630.1223182215318901No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA630.1223182215318901No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA600.11649354431608583No Hit
TACGTAGGGGGCAAGCGTTACTCGGATTTATTGGGCGTAAAGAGAATTAA600.11649354431608583No Hit
TACGTAGGGGGCTAGCGTTACTCGGATTTATTGGGCGTAAAGCGAATGTA600.11649354431608583No Hit
CACGTAGGAGACGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA580.1126104261722163No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGAAAAGGGTGCGCA570.11066886710028154No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA570.11066886710028154No Hit
TACAGGGGTGGCAAGCGGTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA560.10872730802834675No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGATGCGTA540.10484418988447723No Hit
GACGGAGGGTGCTAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA540.10484418988447723No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGCGCA530.10290263081254246No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA520.1009610717406077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG2200.0227.000021
TAGGATC550.0227.000025
TCCCAAG100.001849926227.000029
TACAGGC100.001849926227.000021
CCAGCTC100.001849926227.000023
AGCCAAG450.0227.000029
ATGGCAA202.2638233E-7227.000028
ACCGTCC202.2638233E-7227.000026
GAGCCAA450.0227.000028
ATGCGAG800.0227.000029
CCGTCCA202.2638233E-7227.000027
TACGTAA100.001849926227.000021
GGCCACA100.001849926227.000027
GACGAAC100.001849926227.000021
ACCTCAA100.001849926227.000028
GACCTCA100.001849926227.000027
ACGGATG100.001849926227.000022
GGAGCAA400.0227.000028
AGGAGCC450.0227.000026
ACCAGCT100.001849926227.000022
Produced by FastQC (version 0.11.9)