FastQCFastQC Report
Mon 28 Nov 2022
4061-JW04trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW04trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29416
Sequences flagged as poor quality0
Sequence length232
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA358412.183845526244221No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA24938.474979602937177No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC12454.23239053576285No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG10603.6034810987217845No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA8582.91677998368235No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG7242.461245580636388No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT6142.087299428882241No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT5391.832336143595322No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA4991.6963557247756322No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT4771.6215664944248027No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGGCCAGTG4121.4005983138428066No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATATCAGCGTCAGGACCAGTCCAGAA3761.2782159369050856No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTCAGCGTCAGTATCGAGCCAGAG3501.1898286646722873No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA3131.064046777264074No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA2350.7988849605656786No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC2110.7172967092738646No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG2030.6901006255099266No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA2000.6799020940984498No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT1990.6765025836279576No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTCAGGCCCAGCA1590.5405221648082676No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAACAGCTTAGCA1450.4929290182213761No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC1290.4385368506935001No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT1270.4317378297525157No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC1240.42153929834103887No Hit
CCTGTTTGCTCCCCTGGCTTTCGAATCTCAGCGTCAGGATCAGTCCAGAA1220.41474027740005437No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA1200.4079412564590699No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA1190.4045417459885776No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT1150.3909437041066086No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA1130.3841446831656241No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA1090.37054664128365516No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGT1090.37054664128365516No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTACTGTCCAGGT1070.36374762034267066No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGAA1060.3603481098721784No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGCGTCAGACACAGCCCAGAT980.3331520261082404No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT970.32975251563774816No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTTGGCCAGGT950.32295349469676365No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA950.32295349469676365No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATGAGCGTCAGTAATAGTTTAGTA940.3195539842262714No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA880.2991569214033179No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT860.2923579004623334No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAGTATTGGTCCAGGA810.2753603481098722No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTGCAGACCTAGCG780.2651618166983954No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGAT770.26176230622790314No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGTGTCAGTTACGGACCAGGA750.2549632852869187No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA750.2549632852869187No Hit
CCCGTTTGCTCCCCTAGCTTTCGAGTCTCAGCGTCAGGACTAGTTTAGAG720.24476475387544194No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGGA700.23796573293445744No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATACTCAGCTGGCG700.23796573293445744No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACTGGCCCAGCG680.23116671199347297No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG650.2209681805819962No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGAG620.21076964917051944No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG600.20397062822953496No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATAGGGCCAGAA590.2005711177590427No Hit
CCCGTTTGCTACCTTAGCTTTCGTGTCTGAGCGTCAGAAATGGGCCAGGA590.2005711177590427No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTACCCGTCCAGAT560.19037258634756596No Hit
CCCGTTTGCTACCCTAGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGAG490.1665760130541202No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTACCGTCCAGGT490.1665760130541202No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAGTGGCCCAGGG480.16317650258362795No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTTAGCGTCAGTAATGGACCAAGA470.1597769921131357No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATATCGGTCCAGTA470.1597769921131357No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGTGTCAGGTCAGGCCCAGGT460.15637748164264348No Hit
CCCGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGCTGGGCCAGTG460.15637748164264348No Hit
CCTGTTTGCTCCCCACGCTTTCATGCCTCAGCGTCAGTTACAGTCCAGTA450.1529779711721512No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAG440.14957846070165895No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTCCTCGAATAGAA420.14277943976067448No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTCAGCGCCAGAT420.14277943976067448No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAATCACAGTTTAGCA420.14277943976067448No Hit
CCTGTTCGCTCCCCACGCTTTCGCATATCAGCGTCAATGTTAGGCCAGAG420.14277943976067448No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCGATCACGGCCCGGCG410.13937992929018223No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTCATCGGCCAGAA400.13598041881968997No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGCGTCAGAACCGGTCCAGGT400.13598041881968997No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTTAGGCCCAGCG400.13598041881968997No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA400.13598041881968997No Hit
CCCTTTCGCTCCCCACGCTTTCGTCCCTGAGCGTCAGAACCGTTCTAGTG370.12578188740821322No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG370.12578188740821322No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTCCTGGACCAGCG360.12238237693772097No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA360.12238237693772097No Hit
CCTGTTTGCTACCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA360.12238237693772097No Hit
CCCGTTCGCTCCCCTAGCTTTCGCATCTCAGTGTCAGACACAGTCCAGAA350.11898286646722872No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA350.11898286646722872No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAATGGCCCAGGG340.11558335599673648No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATCTCAGCGTCAGGAACAGTCCAGAA340.11558335599673648No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGTCCAGGA330.1121838455262442No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACGGGCCCAGCG330.1121838455262442No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTATCAGCTTAGCA330.1121838455262442No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGATGCAGTCCAGAA320.10878433505575197No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA320.10878433505575197No Hit
CCCGTTCGCTACCCTAGCTTTCGTGTCTGAGCGTCAGGAACATCCCAGAA320.10878433505575197No Hit
CCCGTTCGCTCCCCGTGCTTTCGCATCTGAGCGTCAGGTCAGGTCCAGCA310.10538482458525972No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATCCGTCCAGAT310.10538482458525972No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA300.10198531411476748No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG300.10198531411476748No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAGCGGTCTAGGA300.10198531411476748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTGC152.0760599E-5226.03
GTTTGCT22000.0226.04
GTTTGCG100.0018704551226.04
GGTTGCT152.0760599E-5226.04
GTTTGAT600.0226.04
TGTTCGC800.0226.03
TGTTTGC20150.0226.03
GGTTCGC1500.0226.03
GGTTTGC202.29742E-7226.03
CCGTTCG2100.0226.02
TGCTCCA152.0760599E-5226.07
CTGTGTG152.0760599E-5226.02
CCATTCG100.0018704551226.02
CTGTTTG20750.0226.02
CGGTTTG202.29742E-7226.02
CGGTTCG1550.0226.02
TTTGCTC18600.0226.05
TTTGCTA3350.0226.05
TGTGCTC152.0760599E-5226.05
TGTGTGC152.0760599E-5226.03
Produced by FastQC (version 0.11.9)