FastQCFastQC Report
Mon 28 Nov 2022
4061-JW05trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW05trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118528
Sequences flagged as poor quality0
Sequence length232
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA5240744.21486906047516No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA85597.221078563714903No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA44303.7375134989200864No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT28482.4028077753779695No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGGCCAGTG15411.3001147408207343No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT13731.158376079913607No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG10300.868992980561555No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA8760.7390658747300216No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATATCGGTCCAGTA8050.6791644168466522No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG7910.6673528617710583No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC7180.6057640388768899No Hit
CCTGTTTGCTACCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA6730.5677983261339092No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA5910.49861636069114473No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGGA5300.4471517278617711No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTCAGGCCCAGCA5220.4404022678185745No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA4950.41762284017278617No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA4670.39399973002159827No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA4480.3779697624190065No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG4430.37375134989200864No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA4240.3577213822894168No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATACTCAGCTGGCG3670.3096314794816415No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTTATTGGCCAGGA3490.2944451943844492No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTTATAGCTTAGCT3490.2944451943844492No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA3460.2919141468682505No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA3230.27250944924406045No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC3200.26997840172786175No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACGGCCCAGAA2980.25141738660907126No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA2950.24888633909287255No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA2860.24129319654427644No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA2700.22779427645788336No Hit
CCCGTTCGCTCCCCTAGCTTTCGCATCTCAGTGTCAGACACAGTCCAGAA2690.2269505939524838No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA2670.22526322894168466No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAATAATGGCTTTGTG2650.22357586393088555No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA2630.22188849892008639No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATGAGCGTCAGTAATAGTTTAGTA2620.22104481641468682No Hit
CCCTTTCGCTCCCCACGCTTTCGTCCCTGAGCGTCAGTATCGTTCCAGCA2500.210920626349892No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATCGGCCAGGA2460.20754589632829373No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT2440.2058585313174946No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT2430.20501484881209503No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACTGGCCCAGCG2380.2007964362850972No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTCAGCGTCAGTATCGAGCCAGAG2330.19657802375809935No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGAAACGGTCCAGGA2280.1923596112311015No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC2240.18898488120950324No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATATCAGCGTCAGGACCAGTCCAGAA2140.18054805615550756No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA2130.179704373650108No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT2010.16958018358531318No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT1950.16451808855291575No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA1920.16198704103671707No Hit
CCGGTTCGTGCCCCCAGCTTTCGTCCCTCACCGTCGAACCCGTTCTGGTA1880.1586123110151188No Hit
CCTGTTTGCTCCCCTGGCTTTCGAATCTCAGCGTCAGGATCAGTCCAGAA1870.1577686285097192No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA1810.15270653347732183No Hit
CCCGTTTGCTACCCTGGCTTTCGCGTCTGAGCGTCAGTTGTGGTCCAGAA1810.15270653347732183No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGAA1750.14764443844492442No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGTA1700.14342602591792655No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG1660.14005129589632828No Hit
CCCGTTTGCTCCCCTAGCTTTCGCATCTTAGCGTCAGGAACGGCCCAGGA1630.1375202483801296No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA1580.13330183585313177No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAATCACAGTTTAGCA1540.1299271058315335No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA1530.1290834233261339No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATGTCGGTCCAGTA1530.1290834233261339No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA1460.12317764578833694No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGGCCAGGA1370.11558450323974083No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACGGTCCAGGA1320.11136609071274299No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAGTATTGGTCCAGGA1320.11136609071274299No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTTACAGCTTAGTG1280.10799136069114472No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG1230.10377294816414687No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTCATCGGCCAGAA1190.1003982181425486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGC7500.0226.03
GTTTGAT4000.0226.04
TCGATCC7350.0226.06
TGTCTGC252.6011548E-9226.03
TGTTCGC2500.0226.03
TGTTCGA7250.0226.03
TGTTTGA3950.0226.03
GGTTCGA100.0018776093226.03
GGTTTGC400.0226.03
CCCATTC850.0226.01
TGATACC302.910383E-11226.07
CCCGTTT4850.0226.01
CCATTCG850.0226.02
TTGATAC302.910383E-11226.06
ATGTTTG100.0018776093226.02
GCTTGCT100.0018776093226.04
CTGTCTG252.6011548E-9226.02
CGGTTTG400.0226.02
CTGTTCG9750.0226.02
TTTGCTA5850.0226.05
Produced by FastQC (version 0.11.9)