FastQCFastQC Report
Mon 28 Nov 2022
4061-JW07trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW07trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126165
Sequences flagged as poor quality0
Sequence length233
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGGAGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA3244825.71870170015456No Hit
AACGAGGGGGACGAGCGTTATTCATCATGACTGGGCGTAAAAGGTTCGTA1275110.106606428090199No Hit
TACGGAGGAGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGTA113779.017556374588832No Hit
TACGAAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA42583.374945507866683No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTA23271.8444100978876867No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA18531.4687116078151627No Hit
TACGGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCCGCA18111.4354218681884834No Hit
TACGAAGGTCCCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGAGCGTA16901.3395157135497167No Hit
TACGAAGGTGGCAAGCGTTGTTCGGATTTACTGGGCGTACAGGGAGCGTA16351.2959220068957318No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA16071.2737288471446122No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA11780.9336979352435304No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA8690.6887805651329608No Hit
TACAGAGGGCTCGAGCGTTAATCGGAATCACTGGGCTTAAAGGGTCCGCA7670.6079340546110252No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA7540.5976300875837197No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCGTGTA6580.5215392541513098No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA5920.469226806166528No Hit
TACGAAGGTGGCAAGCGTTGTTCGGATTCACTGGGCGTACAGGGTGTGTA5840.4628859033804938No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA5550.43990013078112No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATCACTGGGCATAAAGCGCACGTA5250.41612174533349183No Hit
TACGGAGGATGCAAGCGTTATCCGGAATCATTGGGCGTAAAGCGTCCGCA5210.41295129394047475No Hit
GACGAACCGTCCAAACGTTACTCGGAATTACTGGGCTTAAAGGGTGCGTA5190.4113660682439662No Hit
CACGTAGGCACCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA5080.4026473269131693No Hit
TACGGAGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA4940.3915507470376095No Hit
TACAGAGGGTGCAAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA4250.33686046050806484No Hit
TACGAGAGGCCCAAACGTTATTCGGATTTACTGGGCTTAAAGAGTTCGTA4160.3297269448737764No Hit
TACAGAGGGTGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA4160.3297269448737764No Hit
GACAGAGGGTGCAAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA3950.3130820750604367No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA3940.31228946221218246No Hit
TACGGAGGGGGCGAGCGTTATCCGTCATGATTGGGCGTAAAGACTCCGTA3580.2837553996750287No Hit
TACAGAGAGTGCAAACGTTGTTCGGAATTACTGGGCTTAAAGCGCACGTA3570.28296278682677445No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA3510.2782071097372488No Hit
TACGGAGGGGGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA3490.2766218840407403No Hit
TACGGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA3300.26156223992390915No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA2860.22668727460072127No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCACTGGGTTTAAAGGGTGCGTA2850.22589466175246703No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA2690.21321285618039867No Hit
TACGAGGGGGGCGAGCGTTACCCGGAATGATTGGGCGTAAAGGGTCCGTA2660.2108350176356359No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA2590.20528672769785597No Hit
TTCCAGCTCCAATAGCGTATATCTAAGTTGTTGCAGTTAAAAAGCTCGTA2570.20370150200134746No Hit
GACGAACCGTGCGAACGTTGTTCGGAATCACTGGGCTTAAAGGGCGCGTA2490.1973605992153133No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2380.18864185788451632No Hit
AACGTAGGAGGCGAGCGTTATCCGGAGTTACTGGGCGTAAAGAGCGTGTA2320.1838861807949907No Hit
AACGAGGGGGACGAGCGTTATTCACCATGACTGGGCGTAAAAGGTTCGTA2320.1838861807949907No Hit
TTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTA2300.18230095509848215No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA2290.18150834225022788No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGAGTGTA2280.1807157294019736No Hit
TACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA2250.1783378908572108No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA2190.17358221376768518No Hit
TACGTAGGGTGCAGGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2160.17120437522292237No Hit
TACGAAGGTGGCAAGCGTTGTTCGGATTCACTGGGCGTACAGGGAGCGTA2160.17120437522292237No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA2080.1648634724368882No Hit
TACGTAGGCAGCAAACGTTGTTCGGAATTACTGGGCGTAAAGAGTGTGTA2040.16169302104387112No Hit
GACAGAGGGTGCAAACGTTGCTCGGAATCACTGGGCATAAAGCGCACGTA1880.1490112154718028No Hit
GACGGAGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCCGTA1880.1490112154718028No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA1740.13791463559624303No Hit
TACGAGAGGGGCAAGCGTTATTCATCATAACTGGGCGTAAAGTGTCCGTA1710.13553679705148022No Hit
TACGGAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGTA1650.13078111996195457No Hit
TACAGAGGCCTCGAGCGTTGCGCGGAATCACTGGGCTTAAAGGGTGCGTA1630.12919589426544606No Hit
GACGGAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTACGTA1540.12206237863115761No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGCGCACGCA1530.12126976578290334No Hit
TACAGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1530.12126976578290334No Hit
GACGGAGGCTGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA1520.12047715293464908No Hit
GACGAACCACCCAAACGTTATTCGGAATTACTGGGCTTAAAGGGTGCGTA1500.11889192723814053No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA1450.11492886299686919No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGTA1410.11175841160385211No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA1390.11017318590734355No Hit
GACGGAGGATGCAAGCGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA1380.10938057305908928No Hit
TACGTAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA1370.10858796021083503No Hit
AACGTAGGAGACGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA1370.10858796021083503No Hit
TACGTAGGGTCCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1360.10779534736258074No Hit
GACGGAGGAGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGTA1350.10700273451432647No Hit
TACGGAGGAGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCTGTA1350.10700273451432647No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTGCGTA1340.10621012166607222No Hit
AACGTAGGAGACGAACGTTATCCGGAGTTACTGGGCGTAAAGGGCGTGCA1330.10541750881781795No Hit
TACGAATGGGGCAAGCGTTGTTCGGAATCACTGGGCTTAAAGCGTGCGTA1310.10383228312130939No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1280.10145444457654658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG1750.0226.993293
GCCCCAA100.0018532644226.993278
ATGGGGC152.056009E-5226.993276
GTACCGA100.0018532644226.993278
GGTACCG100.0018532644226.993277
CGAACCA202.2771383E-7226.993273
ATGATGC100.0018532644226.993276
CGTATGG152.056009E-5226.993273
TACCAGC100.0018532644226.993271
AGGAGCC100.0018532644226.993276
CGACGGG152.056009E-5226.993273
GGATGCA42200.0226.993277
GGCACCA800.0226.993277
TATGGTG152.056009E-5226.993275
CAGCAAA400.0226.993279
AACCACC152.056009E-5226.993275
TCCCTAG100.0018532644226.993279
AGGACAC252.5356712E-9226.993276
GGATCCG100.0018532644226.993277
TACGGAT100.0018532644226.993271
Produced by FastQC (version 0.11.9)