FastQCFastQC Report
Mon 28 Nov 2022
4061-JW07trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW07trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126165
Sequences flagged as poor quality0
Sequence length232
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA2988623.68802758292712No Hit
CCTGTTTGCTACCCATACTTTCGTTCCTCAACGTCAGTTTTTACTAGAGA1305810.34993857250426No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA121759.650061427495741No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATCACGGGCCAGAT43263.4288431815479727No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTCAGTAATAGCCCAGTA27702.1955375896643283No Hit
CATCTTCGATCCCCTAACTTTCGTTCTTGATTAATGAAAACATCCTTGGT23221.8404470336464154No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTTGCGGCCTAGCA19381.5360836999167757No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG16341.2951293940474775No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGGAGTTGTCCAGTA10170.8060872666745927No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTTAGTAATAGCCCAGTA9560.7577378829310824No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAA8900.7054254349463005No Hit
CCTGTTTGCTACCCATACTTTCGTTCATCAACGTCAGTTTTTACTAGAGA8830.6998771450085206No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTTCTGGTCCAGTA6570.5207466413030555No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGTAAATGTCCAGTA6430.5096500614274958No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCACTACAAGTCCAGCA6270.4969682558554274No Hit
CCCGTTCGCTACCCATGCTTTCGTCCCTGAGCGTCAACAACGGCCCAGCC6210.4922125787659018No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG6010.47636032180081633No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCTTAGTA5860.4644711290770024No Hit
CCCGTTTGCTACCCTAGCTTTCGCACCTCAGCGTCAGATTTGGCCCAGTG5730.45416716204969687No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGTGTCAATATATGTGTAGCC5670.44941148496017125No Hit
CCCGTTTGCTACCTTAGCTTTCGTGCCTCAGCGTCAGAAGAGACCCAGTA5370.4256330995125431No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA5020.3978916498236436No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGTATGTTCCAGAG4600.3646019101969643No Hit
CGTCTTCGAGCCCCCAACTTTCGTTCTTGATCAATGAAAACATCCTTGGC4070.32259342923948797No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTTAGTGTCAGTAATGGCCCAGTA4060.3218008163912337No Hit
CCTGTTTGCTCCCCACGCTTTCCTCCCTCAGCGTCAGTGAAAGAACTCGG4040.32021559069472516No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTATCGTCCAGAA3870.30674117227440256No Hit
CCCAGTTGCTCCCCTAGCTTTCGTCTCTTAGTGTCAGTTTTGGCCCAGCA3630.28771846391630007No Hit
CCCGTTTGCTCCCCTAGCTTTCGCACCTCAGCGTCAGTTGTGGACCAGAG3400.26948836840645185No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG3310.26235485277216347No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTACTGTCCAGGA3260.2583917885308921No Hit
CCTGTTTGATCCCCACGCTTTCGCGCTGCAGCGTCAGAAACTGGCCAGTG2780.2203463718146871No Hit
CCCGTTTGCTCCCCTAGCTTTCGTTCCTCAGCGTCAGAAGAGACCCAGTG2700.21400546902865294No Hit
CCTGTTTGCTCCCTACGCTTTCGTCCCTCAACGTCAGTTTTAGCCCAGAT2680.21242024333214443No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTGCAGCCCAGCA2610.2068719533943645No Hit
CCCGTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA2520.1997384377600761No Hit
CCCCTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA2520.1997384377600761No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTGCACTCTGGTA2510.1989458249118218No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCGCTGTCCAGAA2370.18784924503626202No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTAGCTGTCCAGAA2370.18784924503626202No Hit
CCCGTTCGCTCCCCGTGCTGTCGCGTCTCAGCGTCAGGTCAAGCCCAGGA2330.18467879364324494No Hit
CCTGTTTGCTCCCCACGCTTTCGAGTCTCAGCGTCAGTGTTCGTCCAGTT2260.17913050370546504No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTGTTGTGCCAGGG2190.17358221376768518No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG2180.1727896009194309No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGATCCAGTG2130.16882653667815956No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGTCTAGTA2010.1593151824991083No Hit
CCTGTTTGCTACCCATACTTTCGTCCCTCAACGTCAGTTTATACTAAAGA1980.1569373439543455No Hit
CCTGTTTGATCCCCACGCTTTCGCGCTACAGCGTCAGAAACGAGCCAGTG1970.15614473110609123No Hit
CCCATTCGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTGAGATCCAGCA1970.15614473110609123No Hit
CCCGTTCGCTCCCCATGCTGTCGCGTCTTAGCGTCAGGTACGGCCCAGCG1890.14980382832005706No Hit
CCCGTTCGCTACCCACGCTTTCGTCCCTGAGCGTCAACAACGGCCCAGCC1880.1490112154718028No Hit
CCCGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAATTGTCCAGGG1870.14821860262354852No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG1820.14425553838227717No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTT1810.1434629255340229No Hit
CCCGTTTGCTACCCTAGCTTTCGCACCTCAGCGTCAGAAGTTGCCCAGTG1800.14267031268576863No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTCAGTAATAGCCCAGTA1790.14187769983751436No Hit
CCTGTTTGCTCCCCACACTTTCGCGCATCAGCGTCAGAACCGGTCCAGGT1780.14108508698926012No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTGATTGTCCAGAG1750.13870724844449728No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG1740.13791463559624303No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAATGGACCAGTG1740.13791463559624303No Hit
CCTGTTTGCTCCCCACGCTTTCGCACTTGAGTGTCAGTACTGGACCAGAG1710.13553679705148022No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTATCGGCCCAGCA1700.13474418420322593No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTTTCGGCCCAGAG1690.13395157135497165No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG1680.13315895850671738No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGA1640.1299885071137003No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATTCTGGTCCAGTG1630.12919589426544606No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCTTAGTT1580.12523283002417468No Hit
CCTGTTTGCTACCCGCGCTTTCGCGTCTCAGCGTCAGTACCTGTCCAGGT1580.12523283002417468No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGCGTTAGTAATAGCCCAGTA1560.12364760432766615No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACGGCCCAGAG1540.12206237863115761No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGAT1440.11413625014861492No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG1420.11255102445210637No Hit
CCCATTTGCTACCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA1400.11096579875559782No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA1380.10938057305908928No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACGGCCCAGCT1360.10779534736258074No Hit
CCCGTTCGCTACCCTAGCTTTCGTGCCTCAGCGTCAGAAGAGACCCAGTG1340.10621012166607222No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAAAAATTGCCCAGTT1320.10462489596956366No Hit
CCCGTTTGCTCCCCTAGCTTTCGTGCATCAGCGTCAGTAAAGACCCAGTA1310.10383228312130939No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACTGGTCCAGGT1300.10303967027305513No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGGCCAGTG1280.10145444457654658No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGATCCGGTCCAGGT1280.10145444457654658No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTAATGGTCCAGAG1270.10066183172829232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCA3650.0225.997249
TTTGATC3700.0225.997245
CGTTTGC7450.0225.997223
GGTTGCT152.0923982E-5225.997224
GTTTGAT3800.0225.997224
CTCCCTA650.0225.997229
TGTTCGC750.0225.997223
CCGTTTG7600.0225.997222
GGTTCGC550.0225.997223
CATTTGC39050.0225.997223
CCATTTG39050.0225.997222
ATTCGCT3150.0225.997224
ATTTGCT39150.0225.997224
CGGTTTG202.327597E-7225.997222
CTGTTCG800.0225.997222
CGGTTCG550.0225.997222
GTGTGCT100.0018778216225.997224
GTTGCTA100.0018778216225.997225
CCGGTTC550.0225.997221
CTATTTG100.0018778216225.997222
Produced by FastQC (version 0.11.9)