FastQCFastQC Report
Mon 28 Nov 2022
4061-JW08trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW08trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187475
Sequences flagged as poor quality0
Sequence length233
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA9678151.623416455527405No Hit
GACGGAGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA47582.5379383917855716No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGCACGTA35311.883451126816909No Hit
TACGGAGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA25641.3676490198693159No Hit
TACGGAGGGGGCAAGCGTTACTCGGAATTATTGGGCGTAAAGGGCGCGTA23381.2470996132817709No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA21361.1393519135884784No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA16760.893985864781971No Hit
GACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCTTAAAGGGCGCGTA16120.8598479797306307No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGTACGTA15860.8459794639285237No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA13020.6944925990132017No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA12330.6576876916922256No Hit
TACGTAGGATGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGAGTA11920.6358181090812108No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA11500.6134151220162688No Hit
AACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA10930.583011068142419No Hit
GACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCGTGTA10470.5584744632617682No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGTGTA9070.48379783971196155No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA8740.4661954927323643No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA6300.3360448059741299No Hit
CACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA5920.31577543672489666No Hit
TACAGGGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA5820.3104413921856247No Hit
TACGTAGGATCCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGCGTGTA5450.2907054273903187No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCATGCA5030.2683024403253767No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA4740.25283371116148823No Hit
CACGTAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA4530.2416322176290172No Hit
CACGTAGGAGGCGAGCGTTATCCGGATTCACTGGGCGTAAAGCGCGTGCA4460.23789838645152686No Hit
AACGAGGGGGACGAGCGTTATTCATCATGACTGGGCGTAAAAGGTTCGTA4400.2346979597279637No Hit
AACGTAGGTGGCAAGCGTTATCCGGATTCACTGGGCGTAAAGCGAGTGCA4220.2250966795572743No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGTGTA4190.22349646619549274No Hit
CACGTAGGAGACGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA4130.22029603947192958No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA4050.2160288038405121No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA4040.21549539938658488No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA3980.21229497266302172No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA3940.21016135484731296No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGGGCGCA3910.2085611414855314No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGGGTAAAGGGTGCGCA3840.20482731030804108No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3720.19842645686091478No Hit
TACGGAAGGTGCAAGCGTTAGTCGGAATTACTGGGCGTAAAGGGTGCGTA3570.19042539005200693No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCTCA3390.1808241098813175No Hit
TACGGGGGATGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA3080.1642885718095746No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGCGCGTGCA2980.1589545272703027No Hit
TACGGAGGGTGCAAGCGTTGTTCGGATTCACTGGGTATAAAGGGTGCGTA2970.15842112281637552No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA2950.15735431390852114No Hit
TACAGAGACCTCAAGCGTTATCCGGATTAATTGGGCGTAAAGCGCAGGTA2880.15362048273103082No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGTGTGCA2860.15255367382317642No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA2800.14935324709961328No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA2790.1488198426456861No Hit
TACGTAGGGTGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGCGCGCA2790.1488198426456861No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGTGCGCA2590.1381517535671423No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGTA2570.13708494465928792No Hit
AACGTAGGAGACGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2540.13548473129750632No Hit
GACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA2510.13388451793572476No Hit
CACGTAGGATGCGAGCGTTATTCGGATTTACTGGGCGTAAAGCGCGTGTA2510.13388451793572476No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA2480.1322843045739432No Hit
TACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA2450.13068409121216162No Hit
TACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA2350.1253500466728897No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA2210.11788238431790907No Hit
TACGTAGGGGGCAAGCGTTACTCGGATTTATTGGGCGTAAAGAGAATTAA2160.11521536204827311No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGTA2140.11414855314041872No Hit
AACGTAGGATGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA2140.11414855314041872No Hit
CACGTAGGAGGCGAGCGTTATTCGGATTTACTGGGTGTAAAGCGCGTGTA2120.11308174423256434No Hit
AACGTAGGAAGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGTA2070.1104147219629284No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGCGCA2060.10988131750900121No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA2050.10934791305507402No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA2020.10774769969329244No Hit
TACAGGGGAGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA2000.10668089078543806No Hit
TACGTAGGGAGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGCGTA1950.10401386851580212No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGCGTA1940.1034804640618749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACAAG252.5411282E-9226.994149
GCGCCAC252.5411282E-9226.994146
GAACGAG252.5411282E-9226.994149
GAGCCAA2000.0226.994148
TAGGACG252.5411282E-9226.994145
GAAGCAA100.0018539593226.994138
GGAGCCT100.0018539593226.994137
TACCAGC100.0018539593226.994131
AGGAGCC2150.0226.994136
GGATCCA100.0018539593226.994137
AGGGTTT100.0018539593226.994134
CCGTCCA100.0018539593226.994137
CCCGTTC152.0575675E-5226.994137
GTGCTAA100.0018539593226.994139
TACGAAT100.0018539593226.994131
TACGAAG1400.0226.994131
CCTCAAG400.0226.994139
GTCACCC152.0575675E-5226.994133
GGTGCTA550.0226.994138
ACGTATG350.0226.994132
Produced by FastQC (version 0.11.9)