FastQCFastQC Report
Mon 28 Nov 2022
4061-JW09trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW09trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129940
Sequences flagged as poor quality0
Sequence length233
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA6748751.937047868246886No Hit
GACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCTTAAAGGGCGCGTA23281.7915961212867477No Hit
TACGGAGGGGGCAAGCGTTACTCGGAATTATTGGGCGTAAAGGGCGCGTA23081.776204402031707No Hit
TACGGAGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA20351.5661074342004002No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA20271.5599507464983837No Hit
CACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA15661.2051716176696936No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA14841.1420655687240264No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA11610.8934893027551177No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGCACGTA10640.81883946436817No Hit
GACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCGTGTA8380.644913036786209No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGTGTA8260.6356780052331845No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA8050.6195167000153917No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA7680.5910420193935663No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA6480.4986917038633215No Hit
CACGTAGGAGGCGAGCGTTATCCGGATTCACTGGGCGTAAAGCGCGTGCA5590.43019855317839No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGTGTA5580.429428967215638No Hit
CACGTAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA5480.4217331075881176No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA4990.3840233954132677No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGTACGTA4670.3593966446052024No Hit
CACGTAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA3980.3062952131753117No Hit
TACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA3850.29629059565953514No Hit
TACAGGGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA3770.29013390795751887No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA3760.2893643219947668No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA3580.27551177466523014No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3480.26781591503770974No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA3420.2631983992611975No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA3290.25319378174542095No Hit
CACGTAGGAGACGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA3040.23395413267661996No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA3020.23241496075111592No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGGGCGCA2970.22856703093735573No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2950.22702785901185163No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGTGTGCA2820.21702324149607513No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGCGTA2700.20778820994305064No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGTA2630.20240110820378635No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGGGTAAAGGGTGCGCA2530.19470524857626595No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA2430.18700938894874558No Hit
AACGTAGGAGGCAAGCGTTATCCGAATTTACTGGGTGTAAAGCGCGTGCA2370.18239187317223335No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGATGCGTA2300.17700477143296905No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA2290.17623518547021702No Hit
TACGTATGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA2260.1739264275819609No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA2190.16853932584269662No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2140.16469139602893643No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGTA2020.15545636447591196No Hit
TACGGAGGACCCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA1990.15314760658765586No Hit
TACGTAGGGGGCAAGCGTTACTCGGATTTATTGGGCGTAAAGAGAATTAA1990.15314760658765586No Hit
TACGTAGGGGGCAAGCGTTACTCGGATTCATTGGGCGTAAAGCGAATGTA1890.14545174696013546No Hit
AACGTAGGAAGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGTA1800.1385254732953671No Hit
TACGTAGGATCCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGCGTGTA1790.13775588733261507No Hit
GACAGAGGGTGCGAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1710.13159919963059874No Hit
TACGGGGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA1670.12852085577959058No Hit
AACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA1640.12621209789133445No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1600.12313375404032631No Hit
TACGTAGGGGGCTAGCGTTACTCGGATTTATTGGGCGTAAAGCGAATGTA1600.12313375404032631No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGCA1580.12159458211482223No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGCGCAAGCA1540.11851623826381406No Hit
GACGGAGGGTGCAAGCGTTATTCGGATTTATTGGGCGTAAAGAGCGTGTA1540.11851623826381406No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA1520.11697706633830998No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGCGCA1500.11543789441280591No Hit
TACAGAGGTGTCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGCGTGCA1480.11389872248730183No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGCGCGTGCA1480.11389872248730183No Hit
TACGGGGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1410.10851162074803757No Hit
TACGTAGGGAGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGCGTA1390.10697244882253347No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGTGTA1350.10389410497152532No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGAGCA1350.10389410497152532No Hit
CACGTAGGAATCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA1330.10235493304602125No Hit
GACGGAGGGTGCTAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA1310.10081576112051716No Hit
GACAGAGGTGGCGAGCGTTGTTCGGAATTACTGGGCTTAAAGGGCGAGTA1300.10004617515776512No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGTGCGCA1300.10004617515776512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG450.0227.000023
GATCCAA252.5356712E-9227.000028
GGTCCCA252.5356712E-9227.000027
GGATCCA252.5356712E-9227.000027
AAGGTGC100.0018531628227.000026
CCGTCCA100.0018531628227.000027
ACCTCAA252.5356712E-9227.000028
GTAGCAA100.0018531628227.000028
CCTCAAG252.5356712E-9227.000029
GGTGCCA100.0018531628227.000027
CGTAAGG202.2770473E-7227.000023
ACGTATG400.0227.000022
ACCGTGC202.2770473E-7227.000026
ACCGTCC100.0018531628227.000026
GTCCCAA252.5356712E-9227.000028
AGGATTC100.0018531628227.000026
CACGTAA202.2770473E-7227.000021
TAGGACG202.2770473E-7227.000025
TACAGGT100.0018531628227.000021
CAGAGAC252.5356712E-9227.000023
Produced by FastQC (version 0.11.9)