FastQCFastQC Report
Mon 28 Nov 2022
4061-JW10trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW10trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142480
Sequences flagged as poor quality0
Sequence length233
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA4868334.168304323413814No Hit
TACGGAGGGGGCAAGCGTTACTCGGAATTATTGGGCGTAAAGGGCGCGTA49333.4622403144300957No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGCACGTA39822.7947782144862434No Hit
GACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCTTAAAGGGCGCGTA37872.6579169006176304No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA37652.6424761370016845No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA30392.132930937675463No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGTACGTA26091.8311341942728803No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGTGTA25611.7974452554744527No Hit
CACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA21031.4759966311061201No Hit
TACGGAGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA19411.3622964626614262No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA13230.9285513756316677No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGCA9950.6983436271757439No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA9910.6955362156092083No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGTGTA9470.6646546883773161No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA9060.6358787198203256No Hit
CACGTAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA7350.5158618753509264No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA6160.4323413812464908No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGTGTA5670.397950589556429No Hit
CACGTAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA5650.3965468837731611No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA5620.3944413250982594No Hit
TACGTAGGGGGCAAGCGTTACTCGGATTTATTGGGCGTAAAGAGAATTAA5590.39233576642335766No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA5460.38321167883211676No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGTGTGCA5100.3579449747332959No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGCGCAAGCA5060.35513756316676026No Hit
TACGGGGGGAGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGTGTA5040.3537338573834924No Hit
AACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGCGTGTGCA4980.34952274003368894No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA4700.32987085906793934No Hit
TACGGAGGACCCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA4580.3214486243683324No Hit
GACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCGTGTA4450.3123245367770915No Hit
TACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA4400.308815272318922No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA4190.29407636159460976No Hit
TACGGGGGGAGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGGGCGTGTA3950.27723189219539585No Hit
TACAGAGGTGTCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGCGTGCA3940.27653003930376197No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA3790.2660022459292532No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA3790.2660022459292532No Hit
GACGGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA3720.2610892756878158No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3710.26038742279618193No Hit
TACGGAGGGGGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCGCGCA3570.25056148231330716No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA3550.2491577765300393No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA3530.2477540707467715No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA3500.24564851207186972No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA3450.24213924761370018No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA3400.2386299831555306No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA3320.2330151600224593No Hit
GACAGAGGTGGCGAGCGTTGTTCGGATTTATTGGGCGTAAAGCGAACGCA3290.23090960134755753No Hit
CACGTAGGAGACGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA3260.2288040426726558No Hit
TACAGGGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA3250.22810218978102187No Hit
CACGTAGGAGGCGAGCGTTATCCGGATTCACTGGGCGTAAAGCGCGTGCA3200.22459292532285235No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGCGTA3040.21336327905670974No Hit
TACGTAGGGGGTAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCATGTA3000.21055586749017405No Hit
TACGTAGGGGGCTAGCGTTACTCGGATTTATTGGGCGTAAAGCGAATGTA2980.20915216170690623No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGAGCA2930.20564289724873666No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA2900.20353733857383494No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGGGTAAAGGGTGCGCA2890.202835485682201No Hit
TACGGGGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA2700.18950028074115666No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGAGCGTGTA2570.1803761931499158No Hit
GACGGAGGGTGCTAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA2550.17897248736664795No Hit
TACAGAGGGGGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA2530.17756878158338013No Hit
AACGTAGGAGGCAAGCGTTATCCGAATTTACTGGGTGTAAAGCGCGTGCA2490.17476137001684447No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGCGCA2480.17405951712521056No Hit
TACGTATGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA2430.170550252667041No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGCGCGTA2420.16984839977540708No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGGGCGCA2350.16493542953396967No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA2330.16353172375070185No Hit
TACGTAGGGAGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGCGTA2280.16002245929253228No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2240.15721504772599665No Hit
TACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGTA2210.1551094890510949No Hit
AACGTAGGACGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2160.15160022459292533No Hit
TACGTAGGATCCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGCGTGTA2120.14879281302638966No Hit
TACGGAGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA2120.14879281302638966No Hit
TACGGGGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2100.14738910724312182No Hit
TACGTAGGGTGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGCGCGCA2100.14738910724312182No Hit
AACGTAGGAGGCAAGCGTTATCCGAATTTACTGGGCGTAAAGCGTGTGCA2080.145985401459854No Hit
AACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGTGTGCA1990.1396687254351488No Hit
GACAGAGGGTGCGAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1970.13826501965188098No Hit
CACGTAGGAGTCTAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGTA1950.13686131386861314No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA1800.12633352049410443No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA1780.12492981471083661No Hit
TACGTAGGGAGCGAGCGTTGTCCGAATTTACTGGGCGTAAAGCGCGCGTA1770.12422796181920269No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGTA1650.11580572711959573No Hit
GACGGAGGGTGCAAGCGTTATTCGGATTTATTGGGCGTAAAGAGCGTGTA1640.11510387422796182No Hit
TACAGAGACCTCAAACGTTATCCGGATTTATTGGGCGTAAAGGGTCCGCA1570.11019090398652442No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA1530.10738349241998876No Hit
AACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA1510.10597978663672095No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA1510.10597978663672095No Hit
CACCGGCGGCCCGAGTGGTGATCGTGATTATTGGGTCTAAAGGGTCCGTA1500.10527793374508702No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGTA1470.10317237507018528No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCTCA1460.10247052217855136No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1450.10176866928691747No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA1450.10176866928691747No Hit
TACAGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA1440.10106681639528355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCTA1300.0226.988088
TACGTAT950.0226.988081
AACCGTC450.0226.988075
TACCCGC100.001853642226.988071
CGAACCG800.0226.988073
AGGAGTC400.0226.988076
AACCAGC100.001853642226.988071
GCACCTC100.001853642226.988076
ACCTCAA550.0226.988078
GTAGCAA202.2784116E-7226.988078
GTGCTAA600.0226.988079
GTATGAG100.001853642226.988074
ACGGATG152.0567531E-5226.988072
CCTCAAA350.0226.988079
TTGCGAG202.2784116E-7226.988079
ACAGAGA600.0226.988072
CCCCAAG152.0567531E-5226.988079
CGTAAGG252.5374902E-9226.988073
ACCGTCC500.0226.988076
ACGTACC100.001853642226.988072
Produced by FastQC (version 0.11.9)