FastQCFastQC Report
Mon 28 Nov 2022
4061-JW12trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW12trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163100
Sequences flagged as poor quality0
Sequence length233
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA5841235.81361128142244No Hit
GACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCTTAAAGGGCGCGTA37302.286940527283875No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGCACGTA33732.068056407112201No Hit
TACGGAGGGGGCAAGCGTTACTCGGAATTATTGGGCGTAAAGGGCGCGTA32642.001226241569589No Hit
TACGGAGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA29571.8129981606376455No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA28881.770692826486818No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGTGTA26771.6413243408951566No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA23131.4181483752299204No Hit
CACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA17991.1030042918454936No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA17241.0570202329858982No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA14780.9061925199264255No Hit
CACGTAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA12330.7559779276517474No Hit
TACGTAGGGGGCTAGCGTTACTCGGATTTATTGGGCGTAAAGCGAATGTA12050.7388105456774985No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA11550.7081545064377682No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGTGTA10530.6456161863887186No Hit
TACGTAGGATGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGAGTA10440.640098099325567No Hit
CACGTAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA9010.5524218270999387No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA8390.5144083384426732No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA8380.5137952176578786No Hit
TACGGAGGACCCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA7550.4629061925199264No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGCA7520.4610668301655426No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGCGCGTA7330.44941753525444517No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGTGTA6970.4273451870018393No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA6720.4120171673819743No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA6170.378295524218271No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA5750.3525444512568976No Hit
TACGGGGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA5670.34763948497854075No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA5580.3421213979153893No Hit
CACGTAGGAGACGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA5070.3108522378908645No Hit
TACGTAGGGGGCAAGCGTTACTCGGATTTATTGGGCGTAAAGAGAATTAA4790.2936848559166156No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGATGCGTA4600.2820355610055181No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA4600.2820355610055181No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGAGCGTGTA4390.2691600245248314No Hit
TACGTATGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA4320.26486817903126914No Hit
GACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCGTGTA4290.2630288166768854No Hit
AACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGCGTGTGCA4270.26180257510729615No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA4250.26057633353770693No Hit
CACGTAGGAGGCGAGCGTTATCCGGATTCACTGGGCGTAAAGCGCGTGCA4030.24708767627222564No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGCGCA3820.23421213979153893No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA3800.23298589822194973No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGCGCAAGCA3540.21704475781729No Hit
TACAGAGGTGTCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGCGTGCA3480.21336603310852237No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA3480.21336603310852237No Hit
CACCGGCGGCTCGAGTGGTAACCGTTATTATTGGGTCTAAAGGGTCTGTA3480.21336603310852237No Hit
TACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA3470.21275291232372776No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3450.21152667075413856No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGTGTGCA3370.2066217044757817No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA3360.20600858369098715No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA3340.20478234212139793No Hit
TACGGAGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA3230.19803801348865727No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA3180.19497240956468423No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA3160.19374616799509503No Hit
TACGGAGGGGGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCGCGCA3090.1894543225015328No Hit
GACAGAGGTGGCGAGCGTTGTTCGGATTTATTGGGCGTAAAGCGAACGCA2990.18332311465358675No Hit
AACGTAGGAGGCAAGCGTTATCCGAATTTACTGGGTGTAAAGCGCGTGCA2970.18209687308399755No Hit
CACCGGCGGCCCGAGTGGTGATCGTGATTATTGGGTCTAAAGGGTCCGTA2950.18087063151440833No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2810.17228694052728385No Hit
TACAGGGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA2750.16860821581851623No Hit
GACGGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA2700.16554261189454322No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2660.16309012875536483No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA2640.1618638871857756No Hit
GACGGAGGGTGCTAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA2490.15266707541385655No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA2470.15144083384426732No Hit
TACGTAGGGAGCGAGCGTTGTCCGAATTTACTGGGCGTAAAGCGCGCGTA2420.14837522992029428No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA2360.14469650521152666No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA2350.14408338442673208No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGGGTAAAGGGTGCGCA2300.14101778050275904No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA2270.13917841814837523No Hit
TACGTAGGGAGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGCGTA2250.13795217657878603No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGTA2250.13795217657878603No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGGGCGCA2230.1367259350091968No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA2180.1336603310852238No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2080.12752912323727775No Hit
CACGTAGGAGTCTAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGTA2080.12752912323727775No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA2070.12691600245248313No Hit
TACGTAGGACGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGTCTGTA2060.12630288166768855No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGCGTA2000.1226241569589209No Hit
TACGGGGGGAGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGTGTA1970.12078479460453709No Hit
AACGTAGGACGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA1940.11894543225015328No Hit
TACGGGGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA1920.11771919068056408No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGTA1890.11587982832618027No Hit
TACGTAGGATCCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGCGTGTA1880.11526670754138565No Hit
TACCGGCGGCTCGAGTGGTGGCCACTATTACTGGGCTTAAAGCGTTCGTA1870.11465358675659106No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA1850.11342734518700184No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA1730.10606989576946659No Hit
TACGTAGGGGGTAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCATGTA1720.10545677498467199No Hit
GACAGAGGGTGCGAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1710.10484365419987737No Hit
AACGTAGGAGACGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA1690.10361741263028817No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA1660.10177805027590436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCC302.910383E-11227.000025
CAGCGCC100.0018535958227.000024
TACCCGC100.0018535958227.000021
CGAACCG550.0227.000023
CGTATGA100.0018535958227.000023
AGGAGCC4000.0227.000026
GACGTAT100.0018535958227.000021
GATCCGA3200.0227.000028
ACCTCAA202.2788299E-7227.000028
GCACCAA202.2788299E-7227.000028
CGCCTCG100.0018535958227.000027
GCTCTCC100.0018535958227.000026
GTAGCAA100.0018535958227.000028
GAGACCC100.0018535958227.000025
CATAGGG302.910383E-11227.000023
GGACGCA152.0568663E-5227.000027
GTCCAAG100.0018535958227.000029
CACGTAA252.5393092E-9227.000021
TAGGAGC4000.0227.000025
TAGGACC152.0568663E-5227.000025
Produced by FastQC (version 0.11.9)