FastQCFastQC Report
Mon 28 Nov 2022
4061-JW12trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW12trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163100
Sequences flagged as poor quality0
Sequence length232
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA4951530.358675659104843No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA61633.7786633966891476No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA42802.6241569589209073No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA42382.598405885959534No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG34402.1091354996934397No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT32111.968730839975475No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT29821.8283261802575106No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG28461.7449417535254446No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA15810.9693439607602697No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA12780.7835683629675047No Hit
CCTGTTTGCTCCCCTGGCTTTCGAATCTCAGCGTCAGGATCAGTCCAGAA12210.7486204782342122No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC11960.733292458614347No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT11900.7296137339055794No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA11100.680564071122011No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA8000.4904966278356836No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATACTCAGCTGGCG7510.460453709380748No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATTCGTCCAGAA7000.4291845493562232No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC6450.39546290619251995No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC6380.39117106069895774No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT6360.38994481912936846No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT6030.36971183323114654No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGAA6000.3678724708767627No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA5940.3641937461679951No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATATCAGCGTCAGGACCAGTCCAGAA5650.3464132434089516No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA5380.3298589822194972No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA5290.3243408951563458No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA5250.3218884120171674No Hit
CCTGTTTGCTACCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA5250.3218884120171674No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGGA5210.319435928877989No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA5180.31759656652360513No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAACAGCTTAGCA5170.31698344573881054No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG5030.3083997547516861No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG5020.3077866339668915No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTTGGCCAGGT4910.30104230533415083No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATCGGCCAGGA4890.2998160637645616No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACTGGCCCAGCG4770.29245861434702636No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTCAGGCCCAGCA4680.2869405272838749No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGTTTCAGCGTCAGTAGCAGACCAGAA4620.2832618025751073No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAATAATGGCTTTGTG4580.2808093194359289No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGAT4490.2752912323727774No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA4450.272838749233599No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT4350.266707541385653No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTGCAGACCTAGCG4080.25015328019619865No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG3960.2427958307786634No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA3960.2427958307786634No Hit
CCGGTTCGTGCCCCTAGCTTTCGTCCCTTGCCGTCAGGTCCGTTCTGGTG3840.23543838136112816No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATATCGGTCCAGTA3590.22011036174126303No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTTATTGGCCAGGA3520.2158185162477008No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATGAGCGTCAGTAATAGTTTAGTA3400.20846106683016555No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA3400.20846106683016555No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG3370.2066217044757817No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA3290.2017167381974249No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGTGTCAGAACTGGTCCAGGA3260.19987737584304105No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGTA3170.19435928877988964No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATAGGGCCAGAA3160.19374616799509503No Hit
CCGGTTCGTGCCCCCAGCTTTCGTCCCTCACCGTCGAACCCGTTCTGGTA3090.1894543225015328No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA3040.1863887185775598No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT3030.18577559779276517No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG2980.18270999386879214No Hit
CCTGTTTGCTACCCACGCTTTCGAACATGAGTGTCAGTTACATGCCAGGA2960.18148375229920294No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACGGCCCAGAA2940.18025751072961374No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA2920.17903126916002451No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAATCACAGTTTAGCA2890.1771919068056407No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA2880.17657878602084612No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA2800.17167381974248927No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTGTCGGTCCAGGA2790.17106069895769466No Hit
CCCGTTTGCTACCCTGGCTTTCGCGTCTGAGCGTCAGTTGTGGTCTAGGA2780.17044757817290004No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGT2730.16738197424892703No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATTGCGCCAGTG2690.1649294911097486No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGTCCAGGA2690.1649294911097486No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGGCCAGTG2660.16309012875536483No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAGCGGTCTAGGA2660.16309012875536483No Hit
CCCGTTCGCTCCCCGTGCTTTCGCATCTGAGCGTCAGTATAGGTCCAGCA2550.15634580012262417No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGTGTCAGGTCAGGCCCAGGT2470.15144083384426732No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTCAGCGTCAGTATCGAGCCAGAG2440.1496014714898835No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCGATCACGGCCCGGCG2340.14347026364193746No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTTATAGCTTAGCT2330.14285714285714285No Hit
CCCGTTCGCTACCCATGCTTTCGCGTCTTAGCGTCAGGTACAGCCCAGGG2320.14224402207234826No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC2280.13979153893316984No Hit
CCCGTTTGCTACCCTGGCTTTCGCGTCTGAGCGTCAGTTGTGGTCCAGAA2150.13182096873083998No Hit
CCGGTTTGCTCCCCCAGCTTTCGTCCCTCACTGTCGGAGCCGTTCTGGTG2120.12998160637645617No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGAAACGGTCCAGGA2050.12568976088289394No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAACAGTTTAGTA2000.1226241569589209No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGATGCAGTCCAGAA1910.11710606989576947No Hit
CCCGTTCGCTACCCTAGCTTTCGCACCTCAGCGTCAGGATATGGCCAGAA1910.11710606989576947No Hit
CCCGTTTGCTACCCTAGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGAG1820.11158798283261802No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAGTATTGGTCCAGGA1780.10913549969343961No Hit
CCTGTTTGCTCCCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA1730.10606989576946659No Hit
CCCGTTCGCTACCCTAGCTTTCGTGTCTGAGCGTCAGGAACATCCCAGAA1700.10423053341508277No Hit
CCCGTTTGCTACCCTAGCCTTCGCGTCTGAGCGTCAGGAACATCCCAGAA1700.10423053341508277No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAGCAGTCCAGGA1680.10300429184549358No Hit
CCCGTTCGCTCCCCACGCTTTCGCGCTTCAGCGTCAGTTCTAGGCCAGAG1670.10239117106069896No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAATTTTAGCTTTGTG1650.10116492949110976No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAACGGCCCAGCA1650.10116492949110976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCTA13300.0226.000025
TTCGATC8700.0226.000025
TATTTGC152.0934162E-5226.03
TTGATAC252.6066118E-9226.06
GTTTGAT6300.0226.04
GATACCC252.6066118E-9226.08
GATCGCT152.0934162E-5226.04
CCGATCG152.0934162E-5226.02
CTTTTTG202.3295433E-7226.02
TTGCTCG100.0018782553226.06
CGGTTTG1200.0226.02
GGTTTGC1200.0226.03
CCGCTCG100.0018782553226.02
TTTTTGC202.3295433E-7226.03
CCCGTTT8400.0226.01
TCGCTGC100.0018782553226.06
CTTTGCT202.3295433E-7226.04
CTATTTG152.0934162E-5226.02
GGTTGCT100.0018782553226.04
TTTGATC6050.0226.05
Produced by FastQC (version 0.11.9)