FastQCFastQC Report
Mon 28 Nov 2022
4061-JW13trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW13trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181926
Sequences flagged as poor quality0
Sequence length233
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGGAGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA4548525.001923859151525No Hit
AACGAGGGGGACGAGCGTTATTCATCATGACTGGGCGTAAAAGGTTCGTA131387.2216175807746No Hit
GACGGAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGCA79634.377054406736805No Hit
TACGAAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA61573.3843430845508617No Hit
TACGAAGGTGGCAAGCGTTGTTCGGATTTACTGGGCGTACAGGGAGCGTA39502.171212471004694No Hit
GACGGAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTTCGTA34811.9134153447005926No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA31111.7100359486824313No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA27731.5242461220496244No Hit
TACGGAGGAGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGTA24181.3291118366808483No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA24101.3247144443345096No Hit
TACGAAGGTCCCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGAGCGTA19731.0845068874157624No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA17960.9872145817530203No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA15790.8679353143585854No Hit
CACGTAGGCACCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA15300.8410012862372612No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA14650.80527247342326No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA14390.7909809482976595No Hit
TACAGGGGTCTCGAGCGTTAATCGGAATCACTGGGCTTAAAGGGTGCGTA13980.7684443125226741No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA13400.7365632180117191No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTA13380.7354638699251344No Hit
TACGGAGGATGCAAGCGTTATCCGGAATCATTGGGCGTAAAGCGTCCGCA11270.6194826467904533No Hit
GACGGGGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA11040.6068401437947297No Hit
TACAGAGGGTGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA11030.6062904697514373No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA10500.577157745456944No Hit
GACAGAGGGTGCAAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA10280.5650649165045128No Hit
TACAGAGGCCACAAGCGTTAGGCGGAATCACTGGGCTTAAAGCGTGTGTA10100.5551707837252509No Hit
TACAGAGGGCTCGAGCGTTAATCGGAATCACTGGGCTTAAAGGGTCCGCA8750.48096478788078667No Hit
TACGGAGGGGGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA8680.4771170695777404No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTGCGTA8180.44963336741312404No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCACTGGGTTTAAAGGGTGCGTA7410.4073084660796148No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCGTGTA6790.3732286753954905No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTACGTA6730.3699306311357365No Hit
GACAGAGGGTGCAAACGTTGCTCGGAATCACTGGGCATAAAGCGCACGTA6120.3364005144949045No Hit
TACGAAGGTGGCAAGCGTTGTTCGGATTCACTGGGCGTACAGGGTGTGTA6090.33475149236502755No Hit
TACGAATGGGGCAAGCGTTGTTCGGAATCACTGGGCTTAAAGCGTGCGTA6040.3320031221485659No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGCGCACGCA5920.32540703362905793No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA5740.31551290084979605No Hit
TACGGGGGGGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGGGCGCGTA5490.3017710497674879No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA4950.2720886514297022No Hit
TACGAAGGTGGCAAGCGTTGTTCGGATTCACTGGGCGTACAGGGAGCGTA4900.26934028121324055No Hit
TACGAGAGGGGCAAGCGTTATTCATCATAACTGGGCGTAAAGTGTCCGTA4880.2682409331266559No Hit
TACGGAGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA4610.25339973395776305No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA4580.25175071182788605No Hit
GACGGAGGATGCAAGCGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA4500.24735331948154746No Hit
TACGGAGGGTGCAAACGTTGCTCGGAATCATTGGGCGTAAAGCGCACGTA4490.2468036454382551No Hit
TACAGAGGGTGCAAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA4460.24515462330837812No Hit
TTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTA4280.2352604905291162No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGCACGTA4280.2352604905291162No Hit
GACGGAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTACGTA4170.22921407605290064No Hit
TACAGAGAGTGCAAACGTTGTTCGGAATTACTGGGCTTAAAGCGCACGTA3990.21931994327363874No Hit
TACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA3750.2061277662346229No Hit
AACGTAGGACGCAAGCGTTATCCGGAATTACTGGGCGTAAAGCGCGTGTA3720.20447874410474587No Hit
TACGTAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA3710.20392907006145355No Hit
TACGTAGGCAGCAAGCGTTGTTCGGAGTTACTGGGCGTAAAGAGTACGTA3710.20392907006145355No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA3690.2028297219748689No Hit
TACGAAGGGTGCAAACGTTGCTCGGAATCATTGGGCGTAAAGCGCACGTA3610.19843232962853027No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATCACTGGGCATAAAGCGCACGTA3590.19733298154194562No Hit
CACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA3440.1890878708925607No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGTACGTA3390.18633950067609908No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA3270.17974341215659115No Hit
TACGTAGGGTCCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA3160.17369699768037553No Hit
AACGTAGGAGACGAACGTTATCCGGAGTTACTGGGCGTAAAGGGCGTGCA3140.17259764959379087No Hit
TACAGAGGCCTCGAGCGTTGCGCGGAATCACTGGGCTTAAAGGGTGCGTA3100.17039895342062156No Hit
TACGTAGGCAGCAAACGTTGTTCGGAATTACTGGGCGTAAAGAGTGTGTA3080.16929960533403693No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA3070.1687499312907446No Hit
TACGAGAGGCCCAAACGTTATTCGGATTTACTGGGCTTAAAGAGTTCGTA3010.16545188703099062No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA3000.16490221298769828No Hit
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA2920.16050482064135968No Hit
TACAGAGGCCACAAGCGTTAGTCGGAATCACTGGGCTTAAAGGGTGCGTA2880.15830612446819037No Hit
TACGTAGGGTGCAGGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2760.15171003594868243No Hit
GACGAACCGTCCAAACGTTACTCGGAATTACTGGGCTTAAAGGGTGCGTA2720.14951133977551312No Hit
GACGGAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGTA2680.1473126436023438No Hit
GACGAACCGTGCGAACGTTGTTCGGAATCACTGGGCTTAAAGGGCGCGTA2620.14401459934258984No Hit
GACGGAGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCCGCA2530.1390675329529589No Hit
GACAGAGGGTGCAAACGTTGCTCGGAATCACTGGGCGTAAAGGGCGTGTA2510.13796818486637424No Hit
TACGTATGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA2450.13467014060662028No Hit
AACGTAGGAGACAAGCGTTATCCGGAGTTACTGGGCGTAAAGGGCGTGCA2450.13467014060662028No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA2340.12862372613040468No Hit
TACGAAGGTCCCAAGCGTTGTTCGGATTTACTGGGCGTAAAGCGTCTGTA2280.1253256818706507No Hit
AACGAGGGGGACGAGCGTTATTCACCATGACTGGGCGTAAAAGGTTCGTA2260.12422633378406606No Hit
CACGTACTGTGCGAACGTTATTCGGAATCACTGGGCTTAAAGGGTGCGTA2260.12422633378406606No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGTGCGTA2250.12367665974077373No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA2190.12037861548101975No Hit
AACGTAGGAGGCGAGCGTTATCCGGAGTTACTGGGCGTAAAGAGCGTGTA2090.11488187504809647No Hit
GACGAACCGAGCGAACGTTGTTCGGAATCACTGGGCTTAAAGGGCGCGTA2010.11048448270175786No Hit
GACAGAGGCCACGAACGTTGTTCGGAATTACTGGGCATAAAGCGCACGTA1930.10608709035541924No Hit
AACGTAGGAGACGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA1920.1055374163121269No Hit
TACGGAGGAGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGCA1880.1033387201389576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCAA650.0226.995358
GGCACCA1450.0226.995357
GCTGCAA650.0226.995358
TACGAAT1300.0226.995351
AGGCACC1450.0226.995356
GCCCCGA100.0018538852226.995338
ATCCTAG100.0018538852226.995339
AGGTACC100.0018538852226.995336
CTCACGA252.5393092E-9226.995338
GTACCGA100.0018538852226.995338
GGTACCG100.0018538852226.995337
GCCCAAG100.0018538852226.995339
ACCGGAC100.0018538852226.995336
ACGCAAA202.2797758E-7226.995339
GATCCTA100.0018538852226.995338
CCAATAG2000.0226.995339
ATTGTGC350.0226.995336
AAGGTCC2500.0226.995335
CTCCAAT2000.0226.995337
GTAGGAT350.0226.995334
Produced by FastQC (version 0.11.9)