FastQCFastQC Report
Mon 28 Nov 2022
4061-JW13trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW13trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181926
Sequences flagged as poor quality0
Sequence length232
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA3246617.84571748952871No Hit
CCTGTTTGCTACCCATACTTTCGTTCCTCAACGTCAGTTTTTACTAGAGA141677.7872321713224055No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTCAGTAATAGCCCAGTA105165.7803722392621175No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATCACGGGCCAGAT61953.40523069819597No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTTATGGCCCAGCA57533.1622747710607606No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTTAGTAATAGCCCAGTA46372.5488385387465233No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG41342.2723524949704825No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTTATGGCCCAGTA34061.872189791453668No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGATAAGGTCCAGCA28201.550080802084364No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA28091.5440343876081484No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG20321.1169376559700097No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGTAAATGTCCAGTA17360.9542341391554808No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA16960.9322471774237877No Hit
CCGGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGGAAAACCCCAGCC14400.7915306223409518No Hit
CATCTTCGATCCCCTAACTTTCGTTCTTGATTAATGAAAACATCCTTGGT13960.7673449644360895No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTTCTGGTCCAGTA13620.7486560469641502No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTAGCTGTCCAGAA10640.5848531820630366No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTCATTCCCAGCA10360.5694623088508514No Hit
CCCGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAATTGTCCAGGG10270.5645152424612205No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGAACCGGCCCAGGT10120.5562701318118356No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTATCGTCCAGAA10120.5562701318118356No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAATACTTGTCCAGTG9960.5474753471191584No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCTTAGTA9950.5469256730758659No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGAT9050.49745500917955654No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGTCTAGTA8880.4881105504435869No Hit
CCCGTTCGCTACCCACGCTTTCGTCCCTGAGCGTCAACAACGGCCCAGCC7710.42379868737838466No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACGGCCCAGAG7350.40401042181986085No Hit
CCTGTTTGATCCCCACGCTTTCGCGCTACAGCGTCAGAAACGAGCCAGTG7030.38642085243450636No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG6750.37102997922232117No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCACTACAAGTCCAGCA6580.3616855204863516No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTCAGTAATAGCCCAGTA6330.34794366940404337No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATTCTGGTCCAGTG6290.3457449732308741No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGTATGTTCCAGAG6030.3314534481052736No Hit
CCCGTTTGCTACCCTAGCTTTCGCACCTCAGCGTCAGAAGTTGCCCAGTG5890.323758011499181No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAAAAATTGCCCAGTT5810.31936061915284236No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG5520.30342007189736486No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTTGGCCCAGTT5500.30232072381078023No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA5470.30067170168090324No Hit
CCTGTTTGCTACCCATACTTTCGTCCCTCAACGTCAGTTTATACTAAAGA5430.2984730055077339No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACTGGTCCAGGT5080.27923441399250243No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTT5050.27758539186262543No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCTACGGCCCAGCA4950.2720886514297022No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG4910.26988995525653287No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG4910.26988995525653287No Hit
CCCATTCGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTGAGATCCAGCA4590.25230038587117837No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTTTCGGCCCAGAG4390.24130690500533186No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG4340.2385585347888702No Hit
CGTCTTCGAGCCCCCAACTTTCGTTCTTGATCAATGAAAACATCCTTGGC4150.22811472796631596No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAGTTACAGTCTAGTA4130.2270153798797313No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTGCAGCCCAGCA4070.22371733561997736No Hit
CCCGTTCGCTCCCCATGCTGTCGCGTCTTAGCGTCAGGTACGGCCCAGCG4050.22261798753339268No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATCTAGGTCCAGCA3980.21877026923034643No Hit
CCCGTTCGCTACCCACGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGAA3860.2121741807108385No Hit
CCCGTTCGCTCCCCACGCTTTCGCACCTGAGCGTCAGGCCAAGGCCAGGG3830.2105251585809615No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGGAGTTGTCCAGTA3780.20777678836449986No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTATCGGCCCAGCA3720.20447874410474587No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTAATGGTCCAGAG3640.20008135175840724No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGGCCCAGGT3590.19733298154194562No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT3530.19403493728219168No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGTGTCAATATATGTGTAGCC3410.1874388487626837No Hit
CCCGTTTGCTCCCCTAGCTTTCGCACCTCAGCGTCAGTTGTGGACCAGAG3400.1868891747193914No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGACAGGCCCAGGT3350.18414080450292977No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTGATTGTCCAGAG3330.18304145641634512No Hit
CCTGTTTGATCCCCGCACTTTCGAGCCTCAGTGTCAGTTACAGTTTTGTG3270.17974341215659115No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTCATCGTCCAGCA3090.16984927937732924No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTCAGCGTCAGGTAAGTCCCAGGA2980.16380286490111365No Hit
CCTGTTTGATCCCCACGCTTTCGCGCTGCAGCGTCAGAAACTGGCCAGTG2950.16215384277123668No Hit
CCCCTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA2920.16050482064135968No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATCTTCGTCCAGGT2870.15775645042489803No Hit
CCCGTTCGCTACCCATGCTGTCGCGTCTTAGCGTCAGGTACAGCCCAGCG2840.15610742829502106No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG2740.15061068786209778No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACTTGTCCAGCG2680.1473126436023438No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTACTGTCCAGGA2660.14621329551575915No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGGACAGGCCCAGCC2580.14181590316942055No Hit
CCCGTTCGCTCCCCTGGCCTTCGTGCCTCAGTGTCAGTAACTGCCCAGTG2570.1412662291261282No Hit
CCCGTTTGCTACCTTAGCTTTCGTGCCTCAGCGTCAGAAGAGACCCAGTA2550.14016688103954356No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGATCCAGTG2510.13796818486637424No Hit
CCTTTTCGCTCCCCTGGCCTTCGTCTCTCAGCGTCAGGTGTCGTCCAGAG2480.13631916273649727No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTAGCCCAGGG2430.13357079252003562No Hit
CCCGTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA2420.13302111847674328No Hit
CCCGTTCGCTCCCCGTGCTGTCGCGTCTCAGCGTCAGGTCAAGCCCAGGA2380.130822422303574No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGTGTCAGCAAATGCCCAGCA2350.129173400173697No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCATCGTCCAGAA2330.12807405208711234No Hit
CCTGTTTGCTACCCGCGCTTTCGCGTCTCAGCGTCAGTACCTGTCCAGGT2320.12752437804382No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG2310.1269747040005277No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACGGCCCAGCT2290.12587535591394303No Hit
CCCGTTTGCTACCCTAGCTTTCGCACCTCAGCGTCAGATTTGGCCCAGTG2190.12037861548101975No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG2140.1176302452645581No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG2100.11543154909138881No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGACCAGAA2070.11378252696151182No Hit
CCCGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGAATCGGCCCAGTT2070.11378252696151182No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTTATGGCCCAGCA2010.11048448270175786No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG1960.10773611248529621No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTAATGGCCCAGCA1900.10443806822554225No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGTGTCAGTGTTAACCCAGGG1890.10388839418224993No Hit
CCCGTTTGCTCCCCTAGCTTTCGCACCTCAGCGTCAGTAATGGTCCAGTG1860.10223937205237293No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA1830.10059034992249596No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG1820.10004067587920362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGATC6750.0226.056385
TTGATAC400.0226.056376
GTTTGAT6950.0226.056374
CTTCGAT1250.0226.056374
TTGGCTC302.910383E-11226.056375
TGCTTGA100.001877031226.056373
GGTTTGC1800.0226.056373
GCTTGAT100.001877031226.056374
TGTTCGC1100.0226.056373
CTGTTCG1150.0226.056372
GTTTTCT100.001877031226.056374
CCGGTTC1050.0226.056371
ATTTGAT202.3273788E-7226.056374
TTTTCTA100.001877031226.056375
GTTGCTC202.3273788E-7226.056375
CCTGTTG252.6029738E-9226.056351
CGTTCGC9350.0226.056353
CCTTTTG252.6029738E-9226.056352
CTTTGCT252.6029738E-9226.056354
CTCCCTA302.910383E-11225.994239
Produced by FastQC (version 0.11.9)