FastQCFastQC Report
Mon 28 Nov 2022
4061-JW14trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW14trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169552
Sequences flagged as poor quality0
Sequence length232
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA2613315.412970652071342No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA2362713.93495800698311No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA78114.606846277248278No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA70304.1462206284797585No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT66333.9120741719354535No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTCAGTAATAGCCCAGTA51453.0344673020666226No Hit
CCTGTTTGCTACCCATACTTTCGTTCCTCAACGTCAGTTTTTACTAGAGA46622.749598943097103No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA45142.662310087760687No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA41202.4299329999056334No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT23061.360054732471454No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG20581.213786920826649No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGCGTCAGTACCGGGCCAGTG15760.9295083514202133No Hit
CCTGTTTGCTCCCCACGCCGTCGTGGCTCAGTGTCAGGTCTCGCCCAGAA11000.6487685193922809No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG8860.522553552892328No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG8790.5184250259507407No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGTAAATGTCCAGTA7850.462984807020855No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG7200.424648485420402No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA6980.4116731150325564No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG6650.392210059450788No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACTGGTCCAGGT5930.3497452109087478No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA5830.34384731527790885No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT5620.33146173445314714No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA5430.3202557327545532No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG5310.3131782579975465No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG4740.2795602529017646No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGTTGGTCCAGGG4730.27897046333868075No Hit
CCTGTTTGCTACCCTAGCCTTCGCGCCTCAGCGTCAGATACAGCCCAGAT4720.27838067377559683No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTATCGGCCCAGGG4200.2477116164952345No Hit
CATCTTCGATCCCCTAACTTTCGTTCTTGATTAATGAAAACATCCTTGGT3970.23414645654430497No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG3720.2194017174672077No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGTTGGACCAGGA3660.21586298008870433No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATATTATCCCAGGG3470.2046569783901104No Hit
CCCGTTTGCTCCCCTAGCTTTCGCACACTCAGCGTCAGTTATGGGCCAGA3390.19993866188543927No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG3340.1969897140700198No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTAGCCCAGGG3310.19522034538076813No Hit
CCCGTTTGCTCCCCTGGCCTTCGTGCATGAGCGTCAGGAAATGCCCAGAG3290.19404076625460037No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA3260.19227139756534867No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTTATGGCCCAGTA3110.1834245541190903No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTTTCAGCGTCAGTCACTGTCCAGAA3080.18165518542983863No Hit
CCCATTCGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTGAGATCCAGCA2950.17398792110974803No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGAACCGGTCCAGGT2940.17339813154666414No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATCACGGGCCAGAT2880.16985939416816082No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGTGTCAGGTCAGGCCCAGGT2730.1610125507219024No Hit
CCCGTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA2700.15924318203265075No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGG2650.1562942342172313No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG2640.1557044446541474No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA2630.1551146550910635No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTGAGTCCAGAG2580.15216570727564405No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGTA2410.14213928470321788No Hit
CCCGTTCGCTCCCCATGCTGTCGCGTCTGAGCGTCAGAGTAGGCCCAGCG2390.14095970557705012No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGTGTCAGTTACGGACCAGGA2390.14095970557705012No Hit
CCCCTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA2380.14036991601396623No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG2360.13919033688779842No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT2360.13919033688779842No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG2330.13742096819854677No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTACTGTCCAGGA2310.13624138907237898No Hit
CCCGTTCGCTACCCTAGCTTTCGAGTCTGAGTGTCAGGATCAGACCAGGG2240.13211286213079174No Hit
CCCGTTCGCTCCCCATGCTGTCGCGTCTGAGCGTCAGAGTAGGCCCAGCA2030.11972728130603001No Hit
CCTTTTCGATCCCCACGCTTTCGTGTTTCAGGGTCAGGTGTGCGCCAGCA1910.11264980654902332No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTGCAGCCCAGCA1890.11147022742285552No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTGAGTCCAGGG1870.11029064829668775No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG1850.10911106917051996No Hit
CCCTTTCGCTCCCCTGGCTTTCGTTCCTCAGCGTCAGTTATGGCCCAGCT1840.10852127960743606No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT1800.1061621213551005No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG1800.1061621213551005No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACTCGACCAGAA1790.10557233179201661No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA1780.1049825422289327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA2100.0225.997963
TTTGCTA46350.0225.997965
ATTTGCT32150.0225.997964
GTTTGAT2250.0225.997944
TTGCTAC46400.0225.997946
CCTTTTG152.0936377E-5225.997942
TTTGATC2250.0225.997945
TTTGATA302.910383E-11225.997945
TTGATGC100.0018783722225.997926
TTGCTGC202.3299071E-7225.997926
TTTTTGA400.0225.997923
TCGCTGC202.3299071E-7225.997926
GTTCACT100.0018783722225.997924
TTTGCTG202.3299071E-7225.997925
TTTTGCT100.0018783722225.997924
TGTGTGC100.0018783722225.997923
CTGTGTG100.0018783722225.997922
TGTTCGA1550.0225.997913
GTTTGCT100500.0225.88554
CTGTTTG93850.0225.877532
Produced by FastQC (version 0.11.9)