FastQCFastQC Report
Mon 28 Nov 2022
4061-JW16trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW16trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132588
Sequences flagged as poor quality0
Sequence length232
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA4254432.08736838929617No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT99977.539898029987631No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA67555.09472953811808No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT38762.923341478866866No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA29142.1977856216248832No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG22901.7271547953057593No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGGCCAGTG20861.573294717470661No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT19831.4956104624852928No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA19751.48957673394274No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC17501.3198781186834403No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG13311.003861586267234No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT11450.8635773976528797No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA9750.735360666123631No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA9010.6795486771050171No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC8730.658430627206082No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC8180.616948743476031No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG7440.561136754457417No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA7030.5302138956768335No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG5820.43895375147072135No Hit
CCTGTTTGCTCCCCTGGCTTTCGAATCTCAGCGTCAGGATCAGTCCAGAA5650.4261320783177965No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA5080.38314176245210724No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATATCAGCGTCAGGACCAGTCCAGAA4950.3733369535704589No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTCAGGCCCAGCA4950.3733369535704589No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC4790.3612694964853531No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT4720.3559899840106193No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGAT4660.3514646876037047No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACTGGCCCAGCG3950.297915346788548No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTGCAGACCTAGCG3840.2896189700425378No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAACAGCTTAGCA3800.2866021057712614No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGCCCAGTG3760.2835852414999849No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA3570.26925513621142183No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGAA3550.2677467040757836No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGT3280.24738287024466768No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA3200.24134914170211483No Hit
CCTGTTTGCTACCCTAGCCTTCGCGCCTCAGCGTCAGTCACAGCCCAGAT3140.23682384529520017No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT3070.23154433282046638No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA3000.22626482034573264No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT2880.21721422753190334No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA2810.2119347150571696No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTCAGCGTCAGTATCGAGCCAGAG2780.20967206685371226No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTTGGCCAGGT2740.2066552025824358No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT2730.2059009865146167No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATCGGCCAGGA2730.2059009865146167No Hit
CCCGTTCGCTCCCCGTGCTTTCGCATCTGAGCGTCAGTATAGGTCCAGCA2450.18478293661568165No Hit
CCTGTTTGCTCCCCGCACTTTCGTACCTCAGCGTCAGTCATTGGCCAGGA2270.1712070473949377No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACGGGCCCAGCG2170.16366488671674662No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG2160.1629106706489275No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAGTATTGGTCCAGGA2050.1546142939029173No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA2010.15159742963164086No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATAGGGCCAGAA1890.14254683681781155No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA1860.14028418861435424No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG1850.13952997254653512No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAGTGGCCCAGGG1820.13726732434307778No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA1750.13198781186834405No Hit
CCGGTTCGTGCCCCCAGCTTTCGTCCCTCACCGTCGAACCCGTTCTGGTA1740.13123359580052493No Hit
CCGGTTCGTGCCCCTAGCTTTCGTCCCTTGCCGTCAGGTCCGTTCTGGTG1710.12897094759706762No Hit
CCCGTTCGCTACCCATGCTTTCGCGTCTTAGCGTCAGGTACAGCCCAGGG1690.1274625154614294No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAATCACAGTTTAGCA1670.12595408332579117No Hit
CCTGTTTGCTACCCACGCTTTCGAACATGAGTGTCAGTTACATGCCAGGA1620.12218300298669563No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAACAGTTTAGTA1610.12142878691887651No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGGA1530.11539505837632365No Hit
CCCGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGCTGGGCCAGTG1500.11313241017286632No Hit
CCGGTTTGCTCCCCCAGCTTTCGTCCCTCACTGTCGGAGCCGTTCTGGTG1480.11162397803722811No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGTGTCAGAACTGGTCCAGGA1450.10936132983377078No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATATCGGTCCAGTA1440.10860711376595167No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA1440.10860711376595167No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAATAATGGCTTTGTG1410.10634446556249434No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATTGCGCCAGTG1400.10559024949467523No Hit
CCCGTTCGCTCCCCTAGCTTTCGCATCTCAGTGTCAGACACAGTCCAGAA1380.10408181735903702No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG1370.1033276012912179No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA1360.1025733852233988No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTTCTCGACCAGAA1340.10106495308776058No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATACTCAGCTGGCG1340.10106495308776058No Hit
CCTGTTCGCTCCCCACGCTTTCGCATATCAGCGTCAATGTTAGGCCAGAG1340.10106495308776058No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTCATCGGCCAGAA1330.10031073701994148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTTC4850.0226.000021
TGTTCGC1850.0226.000023
TTTTGCT152.0925343E-5226.04
GTTTGCT104000.0226.04
TCGATCC3100.0226.06
GATACCC100.0018778597226.08
TTCGATC3100.0226.05
TGTTTTC152.0925343E-5226.03
TTTTTGC152.0925343E-5226.03
CCTTTTT202.3279063E-7226.01
TATTTGC100.0018778597226.03
TTGATAC100.0018778597226.06
ATTTGCT100.0018778597226.04
TGCTACC8850.0226.07
CTGTTTT152.0925343E-5226.02
CGGTTTG400.0226.02
TTTGCTG100.0018778597226.05
TTTGCTA8700.0226.05
TTGCTAC8750.0226.06
CTTTTTG152.0925343E-5226.02
Produced by FastQC (version 0.11.9)