FastQCFastQC Report
Mon 28 Nov 2022
4061-JW17trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW17trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74627
Sequences flagged as poor quality0
Sequence length233
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA3766050.4643091642435No Hit
TACGGAGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA15812.118536186634864No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA14741.9751564447183994No Hit
TACGTAGGATGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGAGTA12821.7178769078215659No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA9731.3038176531282244No Hit
TACGGAGGGGGCAAGCGTTACTCGGAATTATTGGGCGTAAAGGGCGCGTA8441.1309579642756644No Hit
GACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCTTAAAGGGCGCGTA7350.984898227183191No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGCACGTA6570.8803784153188524No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA6110.8187385262706527No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA6060.8120385383306309No Hit
TACAGGGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA5880.7879185817465528No Hit
CACGTAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA4520.6056789097779623No Hit
CACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA4440.5949589290739277No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGTGTA4250.5694989749018452No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGGGTAAAGGGTGCGCA4010.537339032789741No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3610.4837391292695673No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA3480.46631916062551093No Hit
CACGTAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA3440.4609591702734935No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGGGCGCA3180.4261192329853806No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA3020.40467927157731115No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGTGTA2590.3470593752931245No Hit
GACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCGTGTA2500.3349993970010854No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGTGTGCA2490.3336593994130811No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2440.32695941147305935No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA2380.3189194259450333No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2120.2840794886569204No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA2030.27201951036488137No Hit
CACGTAGGAGACGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA1890.2532595441328206No Hit
AACGTAGGAGGCAAGCGTTATCCGAATTTACTGGGTGTAAAGCGCGTGCA1830.24521955860479452No Hit
TACAGGGGAGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA1760.2358395754887641No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA1760.2358395754887641No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGATGCGTA1600.21439961408069463No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGCGCA1530.20501963096466427No Hit
TACGGAGGACCCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA1320.17687968161657308No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA1220.16347970573652967No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGTGCGCA1200.16079971056052098No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA1200.16079971056052098No Hit
GACGGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1150.15409972262049929No Hit
TACGTATGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA1150.15409972262049929No Hit
TACGGGGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1150.15409972262049929No Hit
TACGTAGGGGGCAAGCGTTACTCGGATTTATTGGGCGTAAAGAGAATTAA1130.1514197274444906No Hit
TACGGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA1070.14337974191646455No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA1060.1420397443284602No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGCA1050.14069974674045588No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA1050.14069974674045588No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA1040.13935974915245153No Hit
TACAGAGACCTCAAACGTTATCCGGATTTATTGGGCGTAAAGGGTCCGCA1000.13399975880043416No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGGAAAGGGTGCGCA970.12997976603642114No Hit
TACAGGGGTGGCAAGCGGTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA970.12997976603642114No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGCGTA970.12997976603642114No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGTA970.12997976603642114No Hit
TACGGAGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA940.12595977327240812No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA910.12193978050839507No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGTA860.11523979256837337No Hit
CACGTAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCACGTA800.10719980704034732No Hit
TACCGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA790.105859809452343No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA770.1031798142763343No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA750.10049981910032561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGC500.0227.000026
GTTTGCA100.0018515863227.000027
TCCCAAG100.0018515863227.000029
TAGGACG100.0018515863227.000025
ACAGAGA202.2706081E-7227.000022
TACAGGT100.0018515863227.000021
TACAGAG1000.0227.000021
GGTCCCA100.0018515863227.000027
ACGTATG500.0227.000022
ACCGTCC100.0018515863227.000026
GCGCGAG100.0018515863227.000029
CCGTCCA100.0018515863227.000027
GTCCCAA100.0018515863227.000028
TACAAGG100.0018515863227.000021
CGAACCG202.2706081E-7227.000023
GTCTCAA100.0018515863227.000028
CGTATGG500.0227.000023
AGGTCTC100.0018515863227.000026
GACGAAC202.2706081E-7227.000021
AGGTCCC100.0018515863227.000026
Produced by FastQC (version 0.11.9)