FastQCFastQC Report
Mon 28 Nov 2022
4061-JW17trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW17trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74627
Sequences flagged as poor quality0
Sequence length232
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA3551747.5926943331502No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA16362.192236053975103No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA12861.7232368981735833No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT10131.3574175566483981No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT6140.8227585190346658No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG6060.8120385383306309No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT5010.6713387915901752No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC4320.5788789580178756No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGGCCAGTG4070.545379018317767No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG3420.45827917509748484No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA3250.435499216101411No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC2980.3993192812252938No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA2680.35911935358516356No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATATCGGTCCAGTA2580.3457193777051201No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAATCACAGTTTAGCA2250.3014994573009769No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG2210.2961394669489595No Hit
CCTGTTTGCTCCCCACGCTTTCGGGCCTGAGCGTCAGTTCTCGACCAGAA2130.28541948624492475No Hit
CCTGTGTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA2090.28005949589290735No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA2080.27871949830490306No Hit
CCTGTTTGCTCCCCACGCGTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA1770.23717957307676846No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA1760.2358395754887641No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA1680.22511959478472937No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGTA1640.219759604432712No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA1640.219759604432712No Hit
CCTGGTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA1630.21841960684470768No Hit
CCTGTTTGCTACCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA1600.21439961408069463No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTTATTGGCCAGGA1490.1996596406126469No Hit
CCGGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA1490.1996596406126469No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA1470.1969796454366382No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGGA1450.19429965026062954No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA1430.19161965508462084No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA1400.18759966232060782No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTTCTCGACCAGAA1400.18759966232060782No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA1310.17553968402856873No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGGCAGTTCTCGACCAGAA1300.1741996864405644No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGAT1290.17285968885256006No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATCGGCCAGGA1290.17285968885256006No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA1260.16883969608854704No Hit
CCTGTTTGCTCCCCAAGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA1220.16347970573652967No Hit
CCCGTTTGCTACCCTGGCTTTCGCGTCTGAGCGTCAGTTGTGGTCCAGAA1170.15677971779650796No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATGAGCGTCAGTAATAGTTTAGTA1130.1514197274444906No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACGGCCCAGAA1130.1514197274444906No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTCAGGCCCAGCA1070.14337974191646455No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATACTCAGCTGGCG1070.14337974191646455No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA1030.13801975156444718No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT1000.13399975880043416No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCATAA990.1326597612124298No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGCCCAGAA980.13131976362442546No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTTATAGCTTAGCT980.13131976362442546No Hit
CCCGTTCGCTCCCCTAGCTTTCGCATCTCAGTGTCAGACACAGTCCAGAA950.12729977086041244No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC940.12595977327240812No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT940.12595977327240812No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT910.12193978050839507No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA910.12193978050839507No Hit
CCTGTTTGCTCCCCACGATTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA880.11791978774438205No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC860.11523979256837337No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATCGACCAGAA840.11255979739236469No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTCAGCGCCAGAT840.11255979739236469No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG830.11121979980436036No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTCAGCGTCAGTATCGAGCCAGAG800.10719980704034732No Hit
CCCTTTCGCTCCCCACGCTTTCGTCCCTGAGCGTCAGTATCGTTCCAGCA800.10719980704034732No Hit
CCTGCTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA800.10719980704034732No Hit
CCTGTTTTCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA800.10719980704034732No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAATAATGGCTTTGTG770.1031798142763343No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGTA750.10049981910032561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGCT202.3213761E-7225.995324
TGGTCGA100.0018763358225.995323
GGTTGCT400.0225.995324
GTTTGAT1600.0225.995324
GCGATCC100.0018763358225.995326
TTCGATC2900.0225.995325
TGTTCGA2800.0225.995323
TGTTTGA1550.0225.995323
CTGGTCG100.0018763358225.995322
ATTTGCT100.0018763358225.995324
TTGGCTC202.3213761E-7225.995325
GTGCGAT100.0018763358225.995324
CTGTTGG100.0018763358225.995322
CGGTTCG1550.0225.995322
TTTGCTA4000.0225.995325
TGTGCTC500.0225.995325
TGTGCGA100.0018763358225.995323
TGTGTGC350.0225.995323
GGTGTGC100.0018763358225.995323
GTTCGAT2900.0225.995324
Produced by FastQC (version 0.11.9)